Wet pulverization combined with temperature cycling strategy for extraction of Stropharia rugosoannulata protein with attenuating hepatic steatosis on obese mice
Stropharia rugosoannulata is an edible fungus with high protein content and excellent bioactivities. However, the effective extraction of S. rugosoannulata protein has been a challenge, and the amelioration of it against obesity still remain unclear. Herein, S. rugosoannulata protein (SRA) with main...
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| Language: | English |
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Elsevier
2025-01-01
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| Series: | Food Chemistry: X |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590157525000161 |
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| author | Peng Liu Chen Gao Wen Li Wanchao Chen Zhong Zhang Di Wu Tingzhao Li Shuai Sun Yan Yang |
| author_facet | Peng Liu Chen Gao Wen Li Wanchao Chen Zhong Zhang Di Wu Tingzhao Li Shuai Sun Yan Yang |
| author_sort | Peng Liu |
| collection | DOAJ |
| description | Stropharia rugosoannulata is an edible fungus with high protein content and excellent bioactivities. However, the effective extraction of S. rugosoannulata protein has been a challenge, and the amelioration of it against obesity still remain unclear. Herein, S. rugosoannulata protein (SRA) with mainly molecular weight of 15, 40 and 50 kDa, complete amino acid composition and typical α-helix structure, was obtained by wet pulverization combined with temperature cycling strategy. SRA alleviated the pathological progression in obese mice induced by high-fat diet via regulating lipid metabolism in liver and adipose tissues. SRA could obviously improve hepatic steatosis, which may be attributed to inhibiting MAPK/PGC-1α pathway. Moreover, SRA repaired the cross-talk between gut microbiota and liver via modulating intestinal microecology, and restoring the integrity and function of the intestinal barrier in obese mice. This study highlights an efficient and easy strategy for preparing S. rugosoannulata protein and its effects against obesity. |
| format | Article |
| id | doaj-art-36659ca881b94ed69e7ba9c3617b004a |
| institution | Kabale University |
| issn | 2590-1575 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: X |
| spelling | doaj-art-36659ca881b94ed69e7ba9c3617b004a2025-02-12T05:32:21ZengElsevierFood Chemistry: X2590-15752025-01-0125102170Wet pulverization combined with temperature cycling strategy for extraction of Stropharia rugosoannulata protein with attenuating hepatic steatosis on obese micePeng Liu0Chen Gao1Wen Li2Wanchao Chen3Zhong Zhang4Di Wu5Tingzhao Li6Shuai Sun7Yan Yang8Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, P. R. China, National Engineering Research Center of Edible Fungi, Shanghai 201403, ChinaInstitute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, P. R. China, National Engineering Research Center of Edible Fungi, Shanghai 201403, China; University of Shanghai for Science and Technology, Shanghai 200093, ChinaInstitute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, P. R. China, National Engineering Research Center of Edible Fungi, Shanghai 201403, ChinaInstitute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, P. R. China, National Engineering Research Center of Edible Fungi, Shanghai 201403, ChinaInstitute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, P. R. China, National Engineering Research Center of Edible Fungi, Shanghai 201403, ChinaInstitute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, P. R. China, National Engineering Research Center of Edible Fungi, Shanghai 201403, ChinaAmway Innovation & Science Co, Ltd, Shanghai 201203, China; Amway Botanical R&D Center, Wuxi 214115, ChinaAmway Innovation & Science Co, Ltd, Shanghai 201203, China; Corresponding author.Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, P. R. China, National Engineering Research Center of Edible Fungi, Shanghai 201403, China; Corresponding author.Stropharia rugosoannulata is an edible fungus with high protein content and excellent bioactivities. However, the effective extraction of S. rugosoannulata protein has been a challenge, and the amelioration of it against obesity still remain unclear. Herein, S. rugosoannulata protein (SRA) with mainly molecular weight of 15, 40 and 50 kDa, complete amino acid composition and typical α-helix structure, was obtained by wet pulverization combined with temperature cycling strategy. SRA alleviated the pathological progression in obese mice induced by high-fat diet via regulating lipid metabolism in liver and adipose tissues. SRA could obviously improve hepatic steatosis, which may be attributed to inhibiting MAPK/PGC-1α pathway. Moreover, SRA repaired the cross-talk between gut microbiota and liver via modulating intestinal microecology, and restoring the integrity and function of the intestinal barrier in obese mice. This study highlights an efficient and easy strategy for preparing S. rugosoannulata protein and its effects against obesity.http://www.sciencedirect.com/science/article/pii/S2590157525000161Stropharia rugosoannulataObesityProteinHepatic steatosisPreparation strategy |
| spellingShingle | Peng Liu Chen Gao Wen Li Wanchao Chen Zhong Zhang Di Wu Tingzhao Li Shuai Sun Yan Yang Wet pulverization combined with temperature cycling strategy for extraction of Stropharia rugosoannulata protein with attenuating hepatic steatosis on obese mice Food Chemistry: X Stropharia rugosoannulata Obesity Protein Hepatic steatosis Preparation strategy |
| title | Wet pulverization combined with temperature cycling strategy for extraction of Stropharia rugosoannulata protein with attenuating hepatic steatosis on obese mice |
| title_full | Wet pulverization combined with temperature cycling strategy for extraction of Stropharia rugosoannulata protein with attenuating hepatic steatosis on obese mice |
| title_fullStr | Wet pulverization combined with temperature cycling strategy for extraction of Stropharia rugosoannulata protein with attenuating hepatic steatosis on obese mice |
| title_full_unstemmed | Wet pulverization combined with temperature cycling strategy for extraction of Stropharia rugosoannulata protein with attenuating hepatic steatosis on obese mice |
| title_short | Wet pulverization combined with temperature cycling strategy for extraction of Stropharia rugosoannulata protein with attenuating hepatic steatosis on obese mice |
| title_sort | wet pulverization combined with temperature cycling strategy for extraction of stropharia rugosoannulata protein with attenuating hepatic steatosis on obese mice |
| topic | Stropharia rugosoannulata Obesity Protein Hepatic steatosis Preparation strategy |
| url | http://www.sciencedirect.com/science/article/pii/S2590157525000161 |
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