High-throughput robotic isolation of human iPS cell clones reveals frequent homozygous induction of identical genetic manipulations by CRISPR-Cas9

Abstract Background Genome editing in human iPS cells is a powerful approach in regenerative medicine. CRISPR-Cas9 is the most common genome editing tool, but it often induces byproduct insertions and deletions in addition to the desired edits. Therefore, genome editing of iPS cells produces diverse...

Full description

Saved in:
Bibliographic Details
Main Authors: Gou Takahashi, Minato Maeda, Kayoko Shinozaki, Gakuro Harada, Saburo Ito, Yuichiro Miyaoka
Format: Article
Language:English
Published: BMC 2025-06-01
Series:Stem Cell Research & Therapy
Subjects:
Online Access:https://doi.org/10.1186/s13287-025-04414-2
Tags: Add Tag
No Tags, Be the first to tag this record!