The effects of different egg yolk concentrations used with soy bean lecithin-based extender on semen quality to freeze bull semen
<b>Aim:</b> The centrifuged egg yolk (CEY) was added at concentrations of 0.5 or 10% to a defined soy lecithin-based extender (Bioxcell<sup>®</sup>) used to freeze bull semen and their synergistic effects on post-thaw sperm motility, morphological abnormalities and membrane i...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Selcuk University Press
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| Series: | Eurasian Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=558 |
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| Summary: | <b>Aim:</b> The centrifuged egg yolk (CEY) was added at concentrations
of 0.5 or 10% to a defined soy lecithin-based
extender (Bioxcell<sup>®</sup>) used to freeze bull semen and their
synergistic effects on post-thaw sperm motility, morphological
abnormalities and membrane integrity assessed.<p>
<b>Materials and Methods:</b> Ejaculates obtained from each
Simmental bull (n:12) divided in three equal aliquots
and diluted in CEY 5% (B5), 10% (B10) supplemented,
and without any CEY (B0) in soy bean lecithin - based
extender, respectively. Thereafter, they were frozen and
thawed following a standart protocol. Sperm cryosurvival
was evaluated in vitro by microscopic assessments
of post-thaw sperm motility (by means of the CASA),
acrosomal and other abnormalities (head, mid-pieces,
and tail) and plasma membrane integrity (evaluated by
HOST).<p>
<b>Results:</b> In Simmental bulls, semen extended with B5
had significantly higher CASA motility and CASA progressive
motility than those extended with the rest of
extenders after freezing and thawing (p<0.001). There
was no significant difference in VAP, VCL, and ALH
among the three groups (p>0.05). For VSL (p<0.01) and
LIN (p< 0.001), the highest values were obtained from
B10 group. The highest percentages of acrosomal and
other abnormalities were found in semen diluted in B10
(p<0.001). In the group frozen B5, the percentage of
membrane integrity was significantly higher than that of
the other groups (p<0.001).<p>
<b>Conclusion:</b> The use of CEY 5% in combination with soy
bean lecithin significantly improved bull semen freezability. |
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| ISSN: | 1309-6958 2146-1953 |