Antifibrotic Effect of Lactulose on a Methotrexate-Induced Liver Injury Model
The most severe side effect of prolonged MTX treatment is hepatotoxicity. The aim of this study is to investigate the effect of lactulose treatment on MTX-induced hepatotoxicity in a rat model. Twenty-four male rats were included in the study. Sixteen rats were given a single dose of 20 mg/kg MTX to...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Gastroenterology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2017/7942531 |
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| author | Banu Taskin Mümin Alper Erdoğan Gürkan Yiğittürk Damla Günenç Oytun Erbaş |
| author_facet | Banu Taskin Mümin Alper Erdoğan Gürkan Yiğittürk Damla Günenç Oytun Erbaş |
| author_sort | Banu Taskin |
| collection | DOAJ |
| description | The most severe side effect of prolonged MTX treatment is hepatotoxicity. The aim of this study is to investigate the effect of lactulose treatment on MTX-induced hepatotoxicity in a rat model. Twenty-four male rats were included in the study. Sixteen rats were given a single dose of 20 mg/kg MTX to induce liver injury. Eight rats were given no drugs. 16 MTX-given rats were divided into two equal groups. Group 1 subjects were given lactulose 5 g/kg/day, and group 2 subjects were given saline 1 ml/kg/day for 10 days. The rats were then sacrificed to harvest blood and liver tissue samples in order to determine blood and tissue MDA, serum ALT, plasma TNF-α, TGF-β, and PTX3 levels. Histological specimens were examined via light microscopy. Exposure to MTX caused structural and functional hepatotoxicity, as evidenced by relatively worse histopathological scores and increased biochemical marker levels. Lactulose treatment significantly reduced the liver enzyme ALT, plasma TNF-α, TGF-β, PTX3, and MDA levels and also decreased histological changes in the liver tissue with MTX-induced hepatotoxicity in the rat model. We suggest that lactulose has anti-inflammatory and antifibrotic effects on an MTX-induced liver injury model. These effects can be due to the impact of intestinal microbiome. |
| format | Article |
| id | doaj-art-36027a88187249f4b759c03c020a215e |
| institution | Kabale University |
| issn | 1687-6121 1687-630X |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Gastroenterology Research and Practice |
| spelling | doaj-art-36027a88187249f4b759c03c020a215e2025-02-03T01:32:33ZengWileyGastroenterology Research and Practice1687-61211687-630X2017-01-01201710.1155/2017/79425317942531Antifibrotic Effect of Lactulose on a Methotrexate-Induced Liver Injury ModelBanu Taskin0Mümin Alper Erdoğan1Gürkan Yiğittürk2Damla Günenç3Oytun Erbaş4Faculty of Medicine, Department of Dermatology, Istanbul Bilim University, Istanbul, TurkeyFaculty of Medicine, Department of Physiology, Ege University, Izmir, TurkeyFaculty of Medicine, Department of Histology and Embryology, Ege University, Izmir, TurkeyFaculty of Medicine, Department of Internal Medicine, Ege University, Izmir, TurkeyFaculty of Medicine, Department of Physiology, Istanbul Bilim University, Istanbul, TurkeyThe most severe side effect of prolonged MTX treatment is hepatotoxicity. The aim of this study is to investigate the effect of lactulose treatment on MTX-induced hepatotoxicity in a rat model. Twenty-four male rats were included in the study. Sixteen rats were given a single dose of 20 mg/kg MTX to induce liver injury. Eight rats were given no drugs. 16 MTX-given rats were divided into two equal groups. Group 1 subjects were given lactulose 5 g/kg/day, and group 2 subjects were given saline 1 ml/kg/day for 10 days. The rats were then sacrificed to harvest blood and liver tissue samples in order to determine blood and tissue MDA, serum ALT, plasma TNF-α, TGF-β, and PTX3 levels. Histological specimens were examined via light microscopy. Exposure to MTX caused structural and functional hepatotoxicity, as evidenced by relatively worse histopathological scores and increased biochemical marker levels. Lactulose treatment significantly reduced the liver enzyme ALT, plasma TNF-α, TGF-β, PTX3, and MDA levels and also decreased histological changes in the liver tissue with MTX-induced hepatotoxicity in the rat model. We suggest that lactulose has anti-inflammatory and antifibrotic effects on an MTX-induced liver injury model. These effects can be due to the impact of intestinal microbiome.http://dx.doi.org/10.1155/2017/7942531 |
| spellingShingle | Banu Taskin Mümin Alper Erdoğan Gürkan Yiğittürk Damla Günenç Oytun Erbaş Antifibrotic Effect of Lactulose on a Methotrexate-Induced Liver Injury Model Gastroenterology Research and Practice |
| title | Antifibrotic Effect of Lactulose on a Methotrexate-Induced Liver Injury Model |
| title_full | Antifibrotic Effect of Lactulose on a Methotrexate-Induced Liver Injury Model |
| title_fullStr | Antifibrotic Effect of Lactulose on a Methotrexate-Induced Liver Injury Model |
| title_full_unstemmed | Antifibrotic Effect of Lactulose on a Methotrexate-Induced Liver Injury Model |
| title_short | Antifibrotic Effect of Lactulose on a Methotrexate-Induced Liver Injury Model |
| title_sort | antifibrotic effect of lactulose on a methotrexate induced liver injury model |
| url | http://dx.doi.org/10.1155/2017/7942531 |
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