Toward standardized methods in canine vaginal microbiome research: evaluation of storage, host DNA depletion, and database selection

Toward standardized methods in canine vaginal microbiome research: evaluation of storage, host DNA depletion, and database selection

ABSTRACT Standardized methods for investigating the canine vaginal microbiome are not available yet. Data using next-generation sequencing (NGS) are still limited, and methodologies lack consistency. In theory, microbiome results can be significantly affected by factors like sampling technique, stor...

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Bibliographic Details
Main Authors: Lotte Spanoghe, Guillaume Domain, Florin Posastiuc, Amanda Hettiarachchi, Adelaide Panattoni, Sebastiaan Theuns, Filip Van Immerseel, Geert Opsomer, Ann Van Soom, Penelope Banchi
Format: Article
Language:English
Published: American Society for Microbiology 2025-08-01
Series:Microbiology Spectrum
Subjects:
canine vaginal microbiome
microbiome
veterinary microbiology
DNA sequencing
canine reproduction
Online Access:https://journals.asm.org/doi/10.1128/spectrum.00583-25
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https://journals.asm.org/doi/10.1128/spectrum.00583-25

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