Validation of a New Saliva Test for Helicobacter pylori Infection
The purpose of this study was to evaluate a recently introduced saliva test measuring immunoglobulin (Ig) G antibodies to Helicobacter pylori by enzyme-linked immunosorbent assay (ELISA). ELISA has previously been validated against IgG serological tests; however, it is not considered the definitive...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
1996-01-01
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| Series: | Canadian Journal of Gastroenterology |
| Online Access: | http://dx.doi.org/10.1155/1996/825460 |
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| _version_ | 1832555035572043776 |
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| author | OF Bathe AJ Rae P Zetler IGM Cleator |
| author_facet | OF Bathe AJ Rae P Zetler IGM Cleator |
| author_sort | OF Bathe |
| collection | DOAJ |
| description | The purpose of this study was to evaluate a recently introduced saliva test measuring immunoglobulin (Ig) G antibodies to Helicobacter pylori by enzyme-linked immunosorbent assay (ELISA). ELISA has previously been validated against IgG serological tests; however, it is not considered the definitive test for H pylori infection. Using endoscopic antral biopsies as the ’gold standard’ for comparison, the saliva test was validated on 70 patients with upper gastrointestinal symptoms admitted to St Paul’s Hospital Gastroenterology Clinic for gastroduodenoscopy. Thirty-five patients (50%) had histological evidence of gastritis and, by using acridine orange stain, the bacterium was visualized in 25 patients (71%). A biopsy was considered positive when the bacterium was visualized. The saliva test was determined to be 80% sensitive and 80% specific for H pylori infection when the cut-off for a positive test was 0.3 ELISA U/mL. Positive and negative predictive values were 69% and 88%, respectively. More study is required to assess the clinical utility of the test. |
| format | Article |
| id | doaj-art-337b855c0dd04ce1bb39435979ffe5c9 |
| institution | Kabale University |
| issn | 0835-7900 |
| language | English |
| publishDate | 1996-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Canadian Journal of Gastroenterology |
| spelling | doaj-art-337b855c0dd04ce1bb39435979ffe5c92025-02-03T05:49:42ZengWileyCanadian Journal of Gastroenterology0835-79001996-01-01102939610.1155/1996/825460Validation of a New Saliva Test for Helicobacter pylori InfectionOF Bathe0AJ Rae1P Zetler2IGM Cleator3Departments of Surgery and Pathology, St Paul’s Hospital, Vancouver, British Columbia, CanadaDepartments of Surgery and Pathology, St Paul’s Hospital, Vancouver, British Columbia, CanadaDepartments of Surgery and Pathology, St Paul’s Hospital, Vancouver, British Columbia, CanadaDepartments of Surgery and Pathology, St Paul’s Hospital, Vancouver, British Columbia, CanadaThe purpose of this study was to evaluate a recently introduced saliva test measuring immunoglobulin (Ig) G antibodies to Helicobacter pylori by enzyme-linked immunosorbent assay (ELISA). ELISA has previously been validated against IgG serological tests; however, it is not considered the definitive test for H pylori infection. Using endoscopic antral biopsies as the ’gold standard’ for comparison, the saliva test was validated on 70 patients with upper gastrointestinal symptoms admitted to St Paul’s Hospital Gastroenterology Clinic for gastroduodenoscopy. Thirty-five patients (50%) had histological evidence of gastritis and, by using acridine orange stain, the bacterium was visualized in 25 patients (71%). A biopsy was considered positive when the bacterium was visualized. The saliva test was determined to be 80% sensitive and 80% specific for H pylori infection when the cut-off for a positive test was 0.3 ELISA U/mL. Positive and negative predictive values were 69% and 88%, respectively. More study is required to assess the clinical utility of the test.http://dx.doi.org/10.1155/1996/825460 |
| spellingShingle | OF Bathe AJ Rae P Zetler IGM Cleator Validation of a New Saliva Test for Helicobacter pylori Infection Canadian Journal of Gastroenterology |
| title | Validation of a New Saliva Test for Helicobacter pylori Infection |
| title_full | Validation of a New Saliva Test for Helicobacter pylori Infection |
| title_fullStr | Validation of a New Saliva Test for Helicobacter pylori Infection |
| title_full_unstemmed | Validation of a New Saliva Test for Helicobacter pylori Infection |
| title_short | Validation of a New Saliva Test for Helicobacter pylori Infection |
| title_sort | validation of a new saliva test for helicobacter pylori infection |
| url | http://dx.doi.org/10.1155/1996/825460 |
| work_keys_str_mv | AT ofbathe validationofanewsalivatestforhelicobacterpyloriinfection AT ajrae validationofanewsalivatestforhelicobacterpyloriinfection AT pzetler validationofanewsalivatestforhelicobacterpyloriinfection AT igmcleator validationofanewsalivatestforhelicobacterpyloriinfection |