PCR amplication, cloning and sequencing of Protegrin-1 gene of porcine antibacterial peptide
Genome RNA was extracted from bone marrow of pig (Duroc Landrace Tai-hu) and Protegrin-1 (PPG-1) mRNA was amplified using RT-PCR. A DNA fragment about 565 bp in length was obtained and the PCR product was cloned into pGEM-T vector. Protegrin-1 gene was isolated and sequenced from the positive clones...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2004-05-01
|
| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2004.03.0336 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Genome RNA was extracted from bone marrow of pig (Duroc Landrace Tai-hu) and Protegrin-1 (PPG-1) mRNA was amplified using RT-PCR. A DNA fragment about 565 bp in length was obtained and the PCR product was cloned into pGEM-T vector. Protegrin-1 gene was isolated and sequenced from the positive clones screened and the corresponding amino acid sequence was deduced from its nucleotide sequence. Result of sequence analysis showed that this fragment was the partial sequence of PPG-1 gene cDNA. Its homology with PPG-1 from Genebank was up to 98.7%. The deduced sequence for the mature peptide was composed of 18 amino acid residues, which was consistent with that of porcine PPG-1 in Genebank. |
|---|---|
| ISSN: | 1008-9209 2097-5155 |