PCR amplication, cloning and sequencing of Protegrin-1 gene of porcine antibacterial peptide
Genome RNA was extracted from bone marrow of pig (Duroc Landrace Tai-hu) and Protegrin-1 (PPG-1) mRNA was amplified using RT-PCR. A DNA fragment about 565 bp in length was obtained and the PCR product was cloned into pGEM-T vector. Protegrin-1 gene was isolated and sequenced from the positive clones...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2004-05-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2004.03.0336 |
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