Global transcriptional modulation and nutritional status of soybean plants following foliar application of zinc borate as a suspension concentrate fertilizer

Abstract The management of micronutrients, such as boron (B) and zinc (Zn), is critical for plant growth and crop yields. One method of rapid intervention crop management to mitigate nutritional deficiency is the foliar supply of B and Zn. Our study investigates the effect of foliar-supplied B and Z...

Full description

Saved in:
Bibliographic Details
Main Authors: Eloisa Vendemiatti, Rafael Oliveira Moreira, Gabriel Lasmar dos Reis, Inty Omar Hernandez-De Lira, Eugenia Peña-Yewtukhiw, Franz Walter Rieger Hippler, Luis Omar Torres-Dorante, Kiran Pavuluri, Alex Valentine, Vitor L. Nascimento, Vagner Augusto Benedito
Format: Article
Language:English
Published: Nature Portfolio 2025-01-01
Series:Scientific Reports
Subjects:
Online Access:https://doi.org/10.1038/s41598-025-87771-5
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1832571779348955136
author Eloisa Vendemiatti
Rafael Oliveira Moreira
Gabriel Lasmar dos Reis
Inty Omar Hernandez-De Lira
Eugenia Peña-Yewtukhiw
Franz Walter Rieger Hippler
Luis Omar Torres-Dorante
Kiran Pavuluri
Alex Valentine
Vitor L. Nascimento
Vagner Augusto Benedito
author_facet Eloisa Vendemiatti
Rafael Oliveira Moreira
Gabriel Lasmar dos Reis
Inty Omar Hernandez-De Lira
Eugenia Peña-Yewtukhiw
Franz Walter Rieger Hippler
Luis Omar Torres-Dorante
Kiran Pavuluri
Alex Valentine
Vitor L. Nascimento
Vagner Augusto Benedito
author_sort Eloisa Vendemiatti
collection DOAJ
description Abstract The management of micronutrients, such as boron (B) and zinc (Zn), is critical for plant growth and crop yields. One method of rapid intervention crop management to mitigate nutritional deficiency is the foliar supply of B and Zn. Our study investigates the effect of foliar-supplied B and Zn availability on the global transcriptional modulation in soybean (Glycine max). The transcriptional response to B was more widespread compared to Zn. RNA-Seq of leaves under different B levels revealed modulated genes with potential roles in nutritional homeostasis and stress response that may be key to controlling B status in the plant. We also identified putative B transporters whose expression levels were significantly affected by B supplementation with foliar fertilization of plants growing under low B conditions. Furthermore, a gene lacking functional annotation (Glyma.03G180300) emerged as a novel potential marker of B status. Two genes (Glyma.16G118000, Glyma.16G199000) were consistently induced by Zn availability, highlighting their potential as biomarkers for assessing its status in soybean plants. This work advances our understanding of nutritional homeostasis in soybean plants and identifies target genes and potential molecular mechanisms involved in nutritional response. Our study informs fertilizer design targeting specific nutrient transporters, thereby enhancing nutrient efficiency in crops.
format Article
id doaj-art-32e3de9f070540c89a8df8cce6a40980
institution Kabale University
issn 2045-2322
language English
publishDate 2025-01-01
publisher Nature Portfolio
record_format Article
series Scientific Reports
spelling doaj-art-32e3de9f070540c89a8df8cce6a409802025-02-02T12:18:36ZengNature PortfolioScientific Reports2045-23222025-01-0115111810.1038/s41598-025-87771-5Global transcriptional modulation and nutritional status of soybean plants following foliar application of zinc borate as a suspension concentrate fertilizerEloisa Vendemiatti0Rafael Oliveira Moreira1Gabriel Lasmar dos Reis2Inty Omar Hernandez-De Lira3Eugenia Peña-Yewtukhiw4Franz Walter Rieger Hippler5Luis Omar Torres-Dorante6Kiran Pavuluri7Alex Valentine8Vitor L. Nascimento9Vagner Augusto Benedito10 Department of Biology, West Virginia State UniversitySchool of Agriculture and Food Systems, Davis College of Agriculture and Natural Resources, West Virginia UniversitySchool of Agriculture and Food Systems, Davis College of Agriculture and Natural Resources, West Virginia UniversitySchool of Agriculture and Food Systems, Davis College of Agriculture and Natural Resources, West Virginia UniversitySchool of Agriculture and Food Systems, Davis College of Agriculture and Natural Resources, West Virginia UniversityYara Agronomy and R&D - Research Centre Hanninghof, Yara International S.AYara Agronomy and R&D - Research Centre Hanninghof, Yara International S.AInternational Fertilizer Development CenterYara Agronomy and R&D, Yara International S.ADepartment of Biology, Institute of Natural Sciences, Universidade Federal de LavrasSchool of Agriculture and Food Systems, Davis College of Agriculture and Natural Resources, West Virginia UniversityAbstract The management of micronutrients, such as boron (B) and zinc (Zn), is critical for plant growth and crop yields. One method of rapid intervention crop management to mitigate nutritional deficiency is the foliar supply of B and Zn. Our study investigates the effect of foliar-supplied B and Zn availability on the global transcriptional modulation in soybean (Glycine max). The transcriptional response to B was more widespread compared to Zn. RNA-Seq of leaves under different B levels revealed modulated genes with potential roles in nutritional homeostasis and stress response that may be key to controlling B status in the plant. We also identified putative B transporters whose expression levels were significantly affected by B supplementation with foliar fertilization of plants growing under low B conditions. Furthermore, a gene lacking functional annotation (Glyma.03G180300) emerged as a novel potential marker of B status. Two genes (Glyma.16G118000, Glyma.16G199000) were consistently induced by Zn availability, highlighting their potential as biomarkers for assessing its status in soybean plants. This work advances our understanding of nutritional homeostasis in soybean plants and identifies target genes and potential molecular mechanisms involved in nutritional response. Our study informs fertilizer design targeting specific nutrient transporters, thereby enhancing nutrient efficiency in crops.https://doi.org/10.1038/s41598-025-87771-5Cell wall biosynthesisGene expressionFoliar fertilizer uptakeMembrane transportMicronutrient deficiency markersMicronutrient homeostasis
spellingShingle Eloisa Vendemiatti
Rafael Oliveira Moreira
Gabriel Lasmar dos Reis
Inty Omar Hernandez-De Lira
Eugenia Peña-Yewtukhiw
Franz Walter Rieger Hippler
Luis Omar Torres-Dorante
Kiran Pavuluri
Alex Valentine
Vitor L. Nascimento
Vagner Augusto Benedito
Global transcriptional modulation and nutritional status of soybean plants following foliar application of zinc borate as a suspension concentrate fertilizer
Scientific Reports
Cell wall biosynthesis
Gene expression
Foliar fertilizer uptake
Membrane transport
Micronutrient deficiency markers
Micronutrient homeostasis
title Global transcriptional modulation and nutritional status of soybean plants following foliar application of zinc borate as a suspension concentrate fertilizer
title_full Global transcriptional modulation and nutritional status of soybean plants following foliar application of zinc borate as a suspension concentrate fertilizer
title_fullStr Global transcriptional modulation and nutritional status of soybean plants following foliar application of zinc borate as a suspension concentrate fertilizer
title_full_unstemmed Global transcriptional modulation and nutritional status of soybean plants following foliar application of zinc borate as a suspension concentrate fertilizer
title_short Global transcriptional modulation and nutritional status of soybean plants following foliar application of zinc borate as a suspension concentrate fertilizer
title_sort global transcriptional modulation and nutritional status of soybean plants following foliar application of zinc borate as a suspension concentrate fertilizer
topic Cell wall biosynthesis
Gene expression
Foliar fertilizer uptake
Membrane transport
Micronutrient deficiency markers
Micronutrient homeostasis
url https://doi.org/10.1038/s41598-025-87771-5
work_keys_str_mv AT eloisavendemiatti globaltranscriptionalmodulationandnutritionalstatusofsoybeanplantsfollowingfoliarapplicationofzincborateasasuspensionconcentratefertilizer
AT rafaeloliveiramoreira globaltranscriptionalmodulationandnutritionalstatusofsoybeanplantsfollowingfoliarapplicationofzincborateasasuspensionconcentratefertilizer
AT gabriellasmardosreis globaltranscriptionalmodulationandnutritionalstatusofsoybeanplantsfollowingfoliarapplicationofzincborateasasuspensionconcentratefertilizer
AT intyomarhernandezdelira globaltranscriptionalmodulationandnutritionalstatusofsoybeanplantsfollowingfoliarapplicationofzincborateasasuspensionconcentratefertilizer
AT eugeniapenayewtukhiw globaltranscriptionalmodulationandnutritionalstatusofsoybeanplantsfollowingfoliarapplicationofzincborateasasuspensionconcentratefertilizer
AT franzwalterriegerhippler globaltranscriptionalmodulationandnutritionalstatusofsoybeanplantsfollowingfoliarapplicationofzincborateasasuspensionconcentratefertilizer
AT luisomartorresdorante globaltranscriptionalmodulationandnutritionalstatusofsoybeanplantsfollowingfoliarapplicationofzincborateasasuspensionconcentratefertilizer
AT kiranpavuluri globaltranscriptionalmodulationandnutritionalstatusofsoybeanplantsfollowingfoliarapplicationofzincborateasasuspensionconcentratefertilizer
AT alexvalentine globaltranscriptionalmodulationandnutritionalstatusofsoybeanplantsfollowingfoliarapplicationofzincborateasasuspensionconcentratefertilizer
AT vitorlnascimento globaltranscriptionalmodulationandnutritionalstatusofsoybeanplantsfollowingfoliarapplicationofzincborateasasuspensionconcentratefertilizer
AT vagneraugustobenedito globaltranscriptionalmodulationandnutritionalstatusofsoybeanplantsfollowingfoliarapplicationofzincborateasasuspensionconcentratefertilizer