SMRT Gate: A method for validation of synthetic constructs on Pacific Biosciences sequencing platforms

SMRT Gate: A method for validation of synthetic constructs on Pacific Biosciences sequencing platforms

Current DNA assembly methods are prone to sequence errors, requiring rigorous quality control (QC) to identify incorrect assemblies or synthesized constructs. Such errors can lead to misinterpretation of phenotypes. Because of this intrinsic problem, routine QC analysis is generally performed on thr...

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Bibliographic Details
Main Authors: Rosalinda D’Amore, James Johnson, Sam Haldenby, Neil Hall, Margaret Hughes, Ryan Joynson, John G. Kenny, Nicola Patron, Christiane Hertz-Fowler, Anthony Hall
Format: Article
Language:English
Published: Taylor & Francis Group 2017-07-01
Series:BioTechniques
Subjects:
Golden Gate assembly
synthetic biology
next-generation sequencing (NGS)
high-throughput
plasmid QC
Online Access:https://www.future-science.com/doi/10.2144/000114565
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https://www.future-science.com/doi/10.2144/000114565

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