Unlocking the potential of trifluoromethyl pyrrole derivatives in chronic wounds management: rapid synthesis, structural insights, and potent antimicrobial activity

Using a one-pot, three-component approach, we synthesised 25 dihydropyrrol-2-one and two 5-oxo-2,5-dihydrofuran compounds, each featuring two trifluoromethyl groups. This method emphasises timeliness and cost-effectiveness, crucial in drug development. Structural verification was conducted using NMR...

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Main Authors: Rafaela Vrabie, Mariana Pinteala, Cristina M. Al-Matarneh, Ioana C. Marinas, Alina Nicolescu, Sergiu Shova, Mihaela Silion, Mădălina-Diana Gaboreanu, Mariana C. Chifiriuc
Format: Article
Language:English
Published: Taylor & Francis Group 2025-12-01
Series:Journal of Enzyme Inhibition and Medicinal Chemistry
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Online Access:https://www.tandfonline.com/doi/10.1080/14756366.2025.2513406
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author Rafaela Vrabie
Mariana Pinteala
Cristina M. Al-Matarneh
Ioana C. Marinas
Alina Nicolescu
Sergiu Shova
Mihaela Silion
Mădălina-Diana Gaboreanu
Mariana C. Chifiriuc
author_facet Rafaela Vrabie
Mariana Pinteala
Cristina M. Al-Matarneh
Ioana C. Marinas
Alina Nicolescu
Sergiu Shova
Mihaela Silion
Mădălina-Diana Gaboreanu
Mariana C. Chifiriuc
author_sort Rafaela Vrabie
collection DOAJ
description Using a one-pot, three-component approach, we synthesised 25 dihydropyrrol-2-one and two 5-oxo-2,5-dihydrofuran compounds, each featuring two trifluoromethyl groups. This method emphasises timeliness and cost-effectiveness, crucial in drug development. Structural verification was conducted using NMR, FT-IR, MALDI-MS, single-crystal, and powder XRD. The antibacterial and antifungal activities of these compounds were evaluated on yeasts (Candida sp.) and bacteria, including Gram-positive (Staphylococcus aureus, a significant opportunistic pathogen, being more susceptible than S. epidermidis) and Gram-negative strains. Compounds with o-OH and m′-NO2 groups exhibited superior activity, while those with p-OH and m-OMe groups showed slightly lower but still significant activity. For furan structures, Candida albicans displayed greater sensitivity than C. parapsilosis. Additionally, 13 compounds demonstrated haemolysis below 5%, indicating low toxicity.
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issn 1475-6366
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publishDate 2025-12-01
publisher Taylor & Francis Group
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series Journal of Enzyme Inhibition and Medicinal Chemistry
spelling doaj-art-313291dd5d9345ca8ea494d358131f032025-08-20T02:23:12ZengTaylor & Francis GroupJournal of Enzyme Inhibition and Medicinal Chemistry1475-63661475-63742025-12-0140110.1080/14756366.2025.2513406Unlocking the potential of trifluoromethyl pyrrole derivatives in chronic wounds management: rapid synthesis, structural insights, and potent antimicrobial activityRafaela Vrabie0Mariana Pinteala1Cristina M. Al-Matarneh2Ioana C. Marinas3Alina Nicolescu4Sergiu Shova5Mihaela Silion6Mădălina-Diana Gaboreanu7Mariana C. Chifiriuc8Center of Advanced Research in Bionanoconjugates and Biopolymers, “Petru Poni” Institute of Macromolecular Chemistry of Romanian Academy, Iasi, RomaniaCenter of Advanced Research in Bionanoconjugates and Biopolymers, “Petru Poni” Institute of Macromolecular Chemistry of Romanian Academy, Iasi, RomaniaCenter of Advanced Research in Bionanoconjugates and Biopolymers, “Petru Poni” Institute of Macromolecular Chemistry of Romanian Academy, Iasi, RomaniaResearch Institute of the University of Bucharest-ICUB, Bucharest, RomaniaNMR Laboratory, “Petru Poni” Institute of Macromolecular Chemistry of Romanian Academy, Iasi, RomaniaDepartment of Inorganic Polymers, “Petru Poni” Institute of Macromolecular Chemistry of Romanian Academy, Iasi, RomaniaPhysics of Polymers and Polymeric Materials Department, “Petru Poni” Institute of Macromolecular Chemistry, Iasi, RomaniaResearch Institute of the University of Bucharest-ICUB, Bucharest, RomaniaResearch Institute of the University of Bucharest-ICUB, Bucharest, RomaniaUsing a one-pot, three-component approach, we synthesised 25 dihydropyrrol-2-one and two 5-oxo-2,5-dihydrofuran compounds, each featuring two trifluoromethyl groups. This method emphasises timeliness and cost-effectiveness, crucial in drug development. Structural verification was conducted using NMR, FT-IR, MALDI-MS, single-crystal, and powder XRD. The antibacterial and antifungal activities of these compounds were evaluated on yeasts (Candida sp.) and bacteria, including Gram-positive (Staphylococcus aureus, a significant opportunistic pathogen, being more susceptible than S. epidermidis) and Gram-negative strains. Compounds with o-OH and m′-NO2 groups exhibited superior activity, while those with p-OH and m-OMe groups showed slightly lower but still significant activity. For furan structures, Candida albicans displayed greater sensitivity than C. parapsilosis. Additionally, 13 compounds demonstrated haemolysis below 5%, indicating low toxicity.https://www.tandfonline.com/doi/10.1080/14756366.2025.2513406Dihydro-pyrrol-2-oneDoebner-type synthesisantimicrobial activitytrifluoromethylchronic wounds
spellingShingle Rafaela Vrabie
Mariana Pinteala
Cristina M. Al-Matarneh
Ioana C. Marinas
Alina Nicolescu
Sergiu Shova
Mihaela Silion
Mădălina-Diana Gaboreanu
Mariana C. Chifiriuc
Unlocking the potential of trifluoromethyl pyrrole derivatives in chronic wounds management: rapid synthesis, structural insights, and potent antimicrobial activity
Journal of Enzyme Inhibition and Medicinal Chemistry
Dihydro-pyrrol-2-one
Doebner-type synthesis
antimicrobial activity
trifluoromethyl
chronic wounds
title Unlocking the potential of trifluoromethyl pyrrole derivatives in chronic wounds management: rapid synthesis, structural insights, and potent antimicrobial activity
title_full Unlocking the potential of trifluoromethyl pyrrole derivatives in chronic wounds management: rapid synthesis, structural insights, and potent antimicrobial activity
title_fullStr Unlocking the potential of trifluoromethyl pyrrole derivatives in chronic wounds management: rapid synthesis, structural insights, and potent antimicrobial activity
title_full_unstemmed Unlocking the potential of trifluoromethyl pyrrole derivatives in chronic wounds management: rapid synthesis, structural insights, and potent antimicrobial activity
title_short Unlocking the potential of trifluoromethyl pyrrole derivatives in chronic wounds management: rapid synthesis, structural insights, and potent antimicrobial activity
title_sort unlocking the potential of trifluoromethyl pyrrole derivatives in chronic wounds management rapid synthesis structural insights and potent antimicrobial activity
topic Dihydro-pyrrol-2-one
Doebner-type synthesis
antimicrobial activity
trifluoromethyl
chronic wounds
url https://www.tandfonline.com/doi/10.1080/14756366.2025.2513406
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