Quantitative Determination of Lentiviral Vector Particle Numbers by Real-Time PCR
Here, we describe a quantitative, DNAbased, real-time PCR approach to determine the number of lentivirus particles that are present in vector preparations. In this approach, the minus strong-stop cDNA fragment that is present in viral capsids serves as template for PCR. Using this technology, we fou...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2001-09-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/01313st05 |
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