Shift of Microbiota and Modulation of Resistome in the Ceca of Broiler Chicken Fed Berry Pomace Alone or in Combination of a Multienzyme Mixture
Alternative feed additives are being investigated due to the restriction of antibiotics use to decrease antimicrobial resistance (AMR) in food-producing animals. This study investigated the effects of dietary American cranberry (<i>Vaccinium macrocarpon</i>) and wild blueberry (<i>...
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MDPI AG
2025-04-01
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| author | Munene Kithama Yousef I. Hassan Xianhua Yin Joshua Tang Lindsey Clairmont Olimpia Sienkiewicz Kelly Ross Calvin Ho-Fung Lau Dion Lepp Xin Zhao Elijah G. Kiarie Moussa S. Diarra |
| author_facet | Munene Kithama Yousef I. Hassan Xianhua Yin Joshua Tang Lindsey Clairmont Olimpia Sienkiewicz Kelly Ross Calvin Ho-Fung Lau Dion Lepp Xin Zhao Elijah G. Kiarie Moussa S. Diarra |
| author_sort | Munene Kithama |
| collection | DOAJ |
| description | Alternative feed additives are being investigated due to the restriction of antibiotics use to decrease antimicrobial resistance (AMR) in food-producing animals. This study investigated the effects of dietary American cranberry (<i>Vaccinium macrocarpon</i>) and wild blueberry (<i>V. angustifolium</i>) pomaces on the cecal microbiota and resistome profiles as well as the short-chain fatty acid levels. Male broiler chickens Cobb500 were fed a basal diet with either 55 ppm bacitracin methylene disalicylate (BMD); 0.5% (CRP0.5) and 1% (CRP1) cranberry pomace; and 0.5% (LBP0.5) and 1% (LBP1) lowbush blueberry pomace with or without a multienzyme mixture (ENZ). The results showed that at 21 days of age, the total coliform counts decreased in the CRP0.5-fed birds compared to BMD (<i>p</i> < 0.05). The use of pomace significantly increased the abundance of <i>Lactobacillus</i> and <i>Bacteroides</i> regardless of ENZ, while CRP decreased the Proteobacteria phylum abundance. In-feed ENZ tended to increase the relative abundance of genes conferring aminoglycoside resistance. Treatment with CRP0.5 decreased the abundance of <i>cepA</i> genes encoding for macrolide (<i>MACROLIDE)</i> and lincomycin (<i>InuD</i>) resistance while increasing those for tetracycline (<i>tetO</i> and <i>tetX</i>) resistance. These results showed, for the first time, the potential of the studied enzymes in influencing berry pomace’s effects on antimicrobial resistance gene profiles in broilers. |
| format | Article |
| id | doaj-art-2fd5634061dd4f09ad99fffad1e4ec02 |
| institution | Kabale University |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj-art-2fd5634061dd4f09ad99fffad1e4ec022025-08-20T03:47:58ZengMDPI AGMicroorganisms2076-26072025-04-01135104410.3390/microorganisms13051044Shift of Microbiota and Modulation of Resistome in the Ceca of Broiler Chicken Fed Berry Pomace Alone or in Combination of a Multienzyme MixtureMunene Kithama0Yousef I. Hassan1Xianhua Yin2Joshua Tang3Lindsey Clairmont4Olimpia Sienkiewicz5Kelly Ross6Calvin Ho-Fung Lau7Dion Lepp8Xin Zhao9Elijah G. Kiarie10Moussa S. Diarra11Guelph Research and Development Centre, Agriculture and Agri-Food Canada (AAFC), Guelph, ON N1G 5C9, CanadaGuelph Research and Development Centre, Agriculture and Agri-Food Canada (AAFC), Guelph, ON N1G 5C9, CanadaGuelph Research and Development Centre, Agriculture and Agri-Food Canada (AAFC), Guelph, ON N1G 5C9, CanadaGuelph Research and Development Centre, Agriculture and Agri-Food Canada (AAFC), Guelph, ON N1G 5C9, CanadaGuelph Research and Development Centre, Agriculture and Agri-Food Canada (AAFC), Guelph, ON N1G 5C9, CanadaDepartment of Animal Science, McGill University, Montreal, QC H9X 3V9, CanadaSummerland Research and Development Centre, Agriculture and Agri-Food Canada (AAFC), Summerland, BC V0H 1Z0, CanadaOttawa Laboratory (Carling), Canadian Food Inspection Agency, Ottawa, ON K1A 0C6, CanadaGuelph Research and Development Centre, Agriculture and Agri-Food Canada (AAFC), Guelph, ON N1G 5C9, CanadaDepartment of Animal Science, McGill University, Montreal, QC H9X 3V9, CanadaDepartment of Animal Biosciences, University of Guelph, Guelph, ON N1G 2W1, CanadaGuelph Research and Development Centre, Agriculture and Agri-Food Canada (AAFC), Guelph, ON N1G 5C9, CanadaAlternative feed additives are being investigated due to the restriction of antibiotics use to decrease antimicrobial resistance (AMR) in food-producing animals. This study investigated the effects of dietary American cranberry (<i>Vaccinium macrocarpon</i>) and wild blueberry (<i>V. angustifolium</i>) pomaces on the cecal microbiota and resistome profiles as well as the short-chain fatty acid levels. Male broiler chickens Cobb500 were fed a basal diet with either 55 ppm bacitracin methylene disalicylate (BMD); 0.5% (CRP0.5) and 1% (CRP1) cranberry pomace; and 0.5% (LBP0.5) and 1% (LBP1) lowbush blueberry pomace with or without a multienzyme mixture (ENZ). The results showed that at 21 days of age, the total coliform counts decreased in the CRP0.5-fed birds compared to BMD (<i>p</i> < 0.05). The use of pomace significantly increased the abundance of <i>Lactobacillus</i> and <i>Bacteroides</i> regardless of ENZ, while CRP decreased the Proteobacteria phylum abundance. In-feed ENZ tended to increase the relative abundance of genes conferring aminoglycoside resistance. Treatment with CRP0.5 decreased the abundance of <i>cepA</i> genes encoding for macrolide (<i>MACROLIDE)</i> and lincomycin (<i>InuD</i>) resistance while increasing those for tetracycline (<i>tetO</i> and <i>tetX</i>) resistance. These results showed, for the first time, the potential of the studied enzymes in influencing berry pomace’s effects on antimicrobial resistance gene profiles in broilers.https://www.mdpi.com/2076-2607/13/5/1044broiler chickencecaberry pomacemicrobiotaantimicrobial resistance |
| spellingShingle | Munene Kithama Yousef I. Hassan Xianhua Yin Joshua Tang Lindsey Clairmont Olimpia Sienkiewicz Kelly Ross Calvin Ho-Fung Lau Dion Lepp Xin Zhao Elijah G. Kiarie Moussa S. Diarra Shift of Microbiota and Modulation of Resistome in the Ceca of Broiler Chicken Fed Berry Pomace Alone or in Combination of a Multienzyme Mixture Microorganisms broiler chicken ceca berry pomace microbiota antimicrobial resistance |
| title | Shift of Microbiota and Modulation of Resistome in the Ceca of Broiler Chicken Fed Berry Pomace Alone or in Combination of a Multienzyme Mixture |
| title_full | Shift of Microbiota and Modulation of Resistome in the Ceca of Broiler Chicken Fed Berry Pomace Alone or in Combination of a Multienzyme Mixture |
| title_fullStr | Shift of Microbiota and Modulation of Resistome in the Ceca of Broiler Chicken Fed Berry Pomace Alone or in Combination of a Multienzyme Mixture |
| title_full_unstemmed | Shift of Microbiota and Modulation of Resistome in the Ceca of Broiler Chicken Fed Berry Pomace Alone or in Combination of a Multienzyme Mixture |
| title_short | Shift of Microbiota and Modulation of Resistome in the Ceca of Broiler Chicken Fed Berry Pomace Alone or in Combination of a Multienzyme Mixture |
| title_sort | shift of microbiota and modulation of resistome in the ceca of broiler chicken fed berry pomace alone or in combination of a multienzyme mixture |
| topic | broiler chicken ceca berry pomace microbiota antimicrobial resistance |
| url | https://www.mdpi.com/2076-2607/13/5/1044 |
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