Shift of Microbiota and Modulation of Resistome in the Ceca of Broiler Chicken Fed Berry Pomace Alone or in Combination of a Multienzyme Mixture

Shift of Microbiota and Modulation of Resistome in the Ceca of Broiler Chicken Fed Berry Pomace Alone or in Combination of a Multienzyme Mixture

Alternative feed additives are being investigated due to the restriction of antibiotics use to decrease antimicrobial resistance (AMR) in food-producing animals. This study investigated the effects of dietary American cranberry (<i>Vaccinium macrocarpon</i>) and wild blueberry (<i>...

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Main Authors: Munene Kithama, Yousef I. Hassan, Xianhua Yin, Joshua Tang, Lindsey Clairmont, Olimpia Sienkiewicz, Kelly Ross, Calvin Ho-Fung Lau, Dion Lepp, Xin Zhao, Elijah G. Kiarie, Moussa S. Diarra
Format: Article
Language:English
Published: MDPI AG 2025-04-01
Series:Microorganisms
Subjects:
broiler chicken
ceca
berry pomace
microbiota
antimicrobial resistance
Online Access:https://www.mdpi.com/2076-2607/13/5/1044
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Summary:Alternative feed additives are being investigated due to the restriction of antibiotics use to decrease antimicrobial resistance (AMR) in food-producing animals. This study investigated the effects of dietary American cranberry (<i>Vaccinium macrocarpon</i>) and wild blueberry (<i>V. angustifolium</i>) pomaces on the cecal microbiota and resistome profiles as well as the short-chain fatty acid levels. Male broiler chickens Cobb500 were fed a basal diet with either 55 ppm bacitracin methylene disalicylate (BMD); 0.5% (CRP0.5) and 1% (CRP1) cranberry pomace; and 0.5% (LBP0.5) and 1% (LBP1) lowbush blueberry pomace with or without a multienzyme mixture (ENZ). The results showed that at 21 days of age, the total coliform counts decreased in the CRP0.5-fed birds compared to BMD (<i>p</i> < 0.05). The use of pomace significantly increased the abundance of <i>Lactobacillus</i> and <i>Bacteroides</i> regardless of ENZ, while CRP decreased the Proteobacteria phylum abundance. In-feed ENZ tended to increase the relative abundance of genes conferring aminoglycoside resistance. Treatment with CRP0.5 decreased the abundance of <i>cepA</i> genes encoding for macrolide (<i>MACROLIDE)</i> and lincomycin (<i>InuD</i>) resistance while increasing those for tetracycline (<i>tetO</i> and <i>tetX</i>) resistance. These results showed, for the first time, the potential of the studied enzymes in influencing berry pomace’s effects on antimicrobial resistance gene profiles in broilers.
ISSN:2076-2607

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