Evolutionary Insights into IL17A in Lagomorphs
In leporids, IL17A had been implicated in the host defense against extracellular pathogens, such as Francisella tularensis that infects hares and rabbits and causes the zoonotic disease tularemia. Here, we studied IL17A from five lagomorphs, European rabbit, pygmy rabbit, brush rabbit, European brow...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2015/367670 |
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| author | Fabiana Neves Joana Abrantes Tereza Almeida Paulo P. Costa Pedro J. Esteves |
| author_facet | Fabiana Neves Joana Abrantes Tereza Almeida Paulo P. Costa Pedro J. Esteves |
| author_sort | Fabiana Neves |
| collection | DOAJ |
| description | In leporids, IL17A had been implicated in the host defense against extracellular pathogens, such as Francisella tularensis that infects hares and rabbits and causes the zoonotic disease tularemia. Here, we studied IL17A from five lagomorphs, European rabbit, pygmy rabbit, brush rabbit, European brown hare, and American pika. We observed that this protein is highly conserved between these species, with a similarity of 97–99% in leporids and ~88% between leporids and American pika. The exon/intron structure, N-glycosylation sites, and cysteine residues are conserved between lagomorphs. However, at codon 88, one of the interaction sites between IL17A and its receptor IL17RA, there is an Arg>Pro mutation that only occurs in European rabbit and European brown hare. This could induce critical alterations in the IL17A structure and conformation and consequently modify its function. The differences observed between leporids and humans or rodents might also represent important alterations in protein structure and function. In addition, as for other interleukins, IL17A sequences of human and European rabbit are more closely related than the sequences of human and mouse or European rabbit and mouse. This study gives further support to the hypothesis that European rabbit might be a more suitable animal model for studies on human IL17. |
| format | Article |
| id | doaj-art-2eaa6dd44f9c42c89c25bbb3f14a6844 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-2eaa6dd44f9c42c89c25bbb3f14a68442025-02-03T01:07:47ZengWileyMediators of Inflammation0962-93511466-18612015-01-01201510.1155/2015/367670367670Evolutionary Insights into IL17A in LagomorphsFabiana Neves0Joana Abrantes1Tereza Almeida2Paulo P. Costa3Pedro J. Esteves4CIBIO, InBIO-Research Network in Biodiversity and Evolutionary Biology, Universidade do Porto, Campus de Vairão, Rua Padre Armando Quintas, 4485-661 Vairão, PortugalCIBIO, InBIO-Research Network in Biodiversity and Evolutionary Biology, Universidade do Porto, Campus de Vairão, Rua Padre Armando Quintas, 4485-661 Vairão, PortugalCIBIO, InBIO-Research Network in Biodiversity and Evolutionary Biology, Universidade do Porto, Campus de Vairão, Rua Padre Armando Quintas, 4485-661 Vairão, PortugalUnidade Multidisciplinar de Investigação Biomédica (UMIB), Universidade do Porto (UP), Rua de Jorge Viterbo Ferreira, No. 228, 4050-313 Porto, PortugalCIBIO, InBIO-Research Network in Biodiversity and Evolutionary Biology, Universidade do Porto, Campus de Vairão, Rua Padre Armando Quintas, 4485-661 Vairão, PortugalIn leporids, IL17A had been implicated in the host defense against extracellular pathogens, such as Francisella tularensis that infects hares and rabbits and causes the zoonotic disease tularemia. Here, we studied IL17A from five lagomorphs, European rabbit, pygmy rabbit, brush rabbit, European brown hare, and American pika. We observed that this protein is highly conserved between these species, with a similarity of 97–99% in leporids and ~88% between leporids and American pika. The exon/intron structure, N-glycosylation sites, and cysteine residues are conserved between lagomorphs. However, at codon 88, one of the interaction sites between IL17A and its receptor IL17RA, there is an Arg>Pro mutation that only occurs in European rabbit and European brown hare. This could induce critical alterations in the IL17A structure and conformation and consequently modify its function. The differences observed between leporids and humans or rodents might also represent important alterations in protein structure and function. In addition, as for other interleukins, IL17A sequences of human and European rabbit are more closely related than the sequences of human and mouse or European rabbit and mouse. This study gives further support to the hypothesis that European rabbit might be a more suitable animal model for studies on human IL17.http://dx.doi.org/10.1155/2015/367670 |
| spellingShingle | Fabiana Neves Joana Abrantes Tereza Almeida Paulo P. Costa Pedro J. Esteves Evolutionary Insights into IL17A in Lagomorphs Mediators of Inflammation |
| title | Evolutionary Insights into IL17A in Lagomorphs |
| title_full | Evolutionary Insights into IL17A in Lagomorphs |
| title_fullStr | Evolutionary Insights into IL17A in Lagomorphs |
| title_full_unstemmed | Evolutionary Insights into IL17A in Lagomorphs |
| title_short | Evolutionary Insights into IL17A in Lagomorphs |
| title_sort | evolutionary insights into il17a in lagomorphs |
| url | http://dx.doi.org/10.1155/2015/367670 |
| work_keys_str_mv | AT fabiananeves evolutionaryinsightsintoil17ainlagomorphs AT joanaabrantes evolutionaryinsightsintoil17ainlagomorphs AT terezaalmeida evolutionaryinsightsintoil17ainlagomorphs AT paulopcosta evolutionaryinsightsintoil17ainlagomorphs AT pedrojesteves evolutionaryinsightsintoil17ainlagomorphs |