Evolutionary Insights into IL17A in Lagomorphs

In leporids, IL17A had been implicated in the host defense against extracellular pathogens, such as Francisella tularensis that infects hares and rabbits and causes the zoonotic disease tularemia. Here, we studied IL17A from five lagomorphs, European rabbit, pygmy rabbit, brush rabbit, European brow...

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Main Authors: Fabiana Neves, Joana Abrantes, Tereza Almeida, Paulo P. Costa, Pedro J. Esteves
Format: Article
Language:English
Published: Wiley 2015-01-01
Series:Mediators of Inflammation
Online Access:http://dx.doi.org/10.1155/2015/367670
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author Fabiana Neves
Joana Abrantes
Tereza Almeida
Paulo P. Costa
Pedro J. Esteves
author_facet Fabiana Neves
Joana Abrantes
Tereza Almeida
Paulo P. Costa
Pedro J. Esteves
author_sort Fabiana Neves
collection DOAJ
description In leporids, IL17A had been implicated in the host defense against extracellular pathogens, such as Francisella tularensis that infects hares and rabbits and causes the zoonotic disease tularemia. Here, we studied IL17A from five lagomorphs, European rabbit, pygmy rabbit, brush rabbit, European brown hare, and American pika. We observed that this protein is highly conserved between these species, with a similarity of 97–99% in leporids and ~88% between leporids and American pika. The exon/intron structure, N-glycosylation sites, and cysteine residues are conserved between lagomorphs. However, at codon 88, one of the interaction sites between IL17A and its receptor IL17RA, there is an Arg>Pro mutation that only occurs in European rabbit and European brown hare. This could induce critical alterations in the IL17A structure and conformation and consequently modify its function. The differences observed between leporids and humans or rodents might also represent important alterations in protein structure and function. In addition, as for other interleukins, IL17A sequences of human and European rabbit are more closely related than the sequences of human and mouse or European rabbit and mouse. This study gives further support to the hypothesis that European rabbit might be a more suitable animal model for studies on human IL17.
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institution Kabale University
issn 0962-9351
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language English
publishDate 2015-01-01
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series Mediators of Inflammation
spelling doaj-art-2eaa6dd44f9c42c89c25bbb3f14a68442025-02-03T01:07:47ZengWileyMediators of Inflammation0962-93511466-18612015-01-01201510.1155/2015/367670367670Evolutionary Insights into IL17A in LagomorphsFabiana Neves0Joana Abrantes1Tereza Almeida2Paulo P. Costa3Pedro J. Esteves4CIBIO, InBIO-Research Network in Biodiversity and Evolutionary Biology, Universidade do Porto, Campus de Vairão, Rua Padre Armando Quintas, 4485-661 Vairão, PortugalCIBIO, InBIO-Research Network in Biodiversity and Evolutionary Biology, Universidade do Porto, Campus de Vairão, Rua Padre Armando Quintas, 4485-661 Vairão, PortugalCIBIO, InBIO-Research Network in Biodiversity and Evolutionary Biology, Universidade do Porto, Campus de Vairão, Rua Padre Armando Quintas, 4485-661 Vairão, PortugalUnidade Multidisciplinar de Investigação Biomédica (UMIB), Universidade do Porto (UP), Rua de Jorge Viterbo Ferreira, No. 228, 4050-313 Porto, PortugalCIBIO, InBIO-Research Network in Biodiversity and Evolutionary Biology, Universidade do Porto, Campus de Vairão, Rua Padre Armando Quintas, 4485-661 Vairão, PortugalIn leporids, IL17A had been implicated in the host defense against extracellular pathogens, such as Francisella tularensis that infects hares and rabbits and causes the zoonotic disease tularemia. Here, we studied IL17A from five lagomorphs, European rabbit, pygmy rabbit, brush rabbit, European brown hare, and American pika. We observed that this protein is highly conserved between these species, with a similarity of 97–99% in leporids and ~88% between leporids and American pika. The exon/intron structure, N-glycosylation sites, and cysteine residues are conserved between lagomorphs. However, at codon 88, one of the interaction sites between IL17A and its receptor IL17RA, there is an Arg>Pro mutation that only occurs in European rabbit and European brown hare. This could induce critical alterations in the IL17A structure and conformation and consequently modify its function. The differences observed between leporids and humans or rodents might also represent important alterations in protein structure and function. In addition, as for other interleukins, IL17A sequences of human and European rabbit are more closely related than the sequences of human and mouse or European rabbit and mouse. This study gives further support to the hypothesis that European rabbit might be a more suitable animal model for studies on human IL17.http://dx.doi.org/10.1155/2015/367670
spellingShingle Fabiana Neves
Joana Abrantes
Tereza Almeida
Paulo P. Costa
Pedro J. Esteves
Evolutionary Insights into IL17A in Lagomorphs
Mediators of Inflammation
title Evolutionary Insights into IL17A in Lagomorphs
title_full Evolutionary Insights into IL17A in Lagomorphs
title_fullStr Evolutionary Insights into IL17A in Lagomorphs
title_full_unstemmed Evolutionary Insights into IL17A in Lagomorphs
title_short Evolutionary Insights into IL17A in Lagomorphs
title_sort evolutionary insights into il17a in lagomorphs
url http://dx.doi.org/10.1155/2015/367670
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AT joanaabrantes evolutionaryinsightsintoil17ainlagomorphs
AT terezaalmeida evolutionaryinsightsintoil17ainlagomorphs
AT paulopcosta evolutionaryinsightsintoil17ainlagomorphs
AT pedrojesteves evolutionaryinsightsintoil17ainlagomorphs