A rapid and robust protocol for generating loss-of-function alleles in pluripotent stem cells

A rapid and robust protocol for generating loss-of-function alleles in pluripotent stem cells

Summary: The generation of loss-of-function alleles in human pluripotent stem cells (hPSCs) is used to interrogate gene function and validate reagents; however, identifying clones harboring true loss-of-function alleles remains inefficient. To address this, we present BOLT (barcoded oligos for loss-...

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Bibliographic Details
Main Authors: Erdene Baljinnyam, Laura Grisanti, Shalini Tattari, Chiara Pedicone, Bhavana Shewale, Nicole Dubois, Alison M. Goate, William G. Kerr, Samuele G. Marro
Format: Article
Language:English
Published: Elsevier 2025-09-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell culture
CRISPR
Genomics
Molecular Biology
Sequencing
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725003223
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http://www.sciencedirect.com/science/article/pii/S2666166725003223

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