Oxygenase Enzyme Activity and Compound Profile in Hydrocarbon Bioremediation by Pseudomonas aeruginosa and Rhodococcus erythropolis Consortium
Abstract Hydrocarbon contamination is one of the most significant environmental problems, including in the marine environment. Several methods, such as chemical and physical remediation, have been constructed to remediate pollutants but remain side effects and have a relatively high cost. Bioremed...
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Faculty of Fisheries and Marine Universitas Airlangga
2023-12-01
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| Series: | Jurnal Ilmiah Perikanan dan Kelautan |
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| Online Access: | https://e-journal.unair.ac.id/JIPK/article/view/45528 |
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| author | Adi Tiya Yanuar Abd. Aziz Amin Lutfi Ni'matus Salamah Frentina Murty Sujadi Ekwan Nofa Wiratno Citra Satrya Utama Dewi Andi Kurniawan |
| author_facet | Adi Tiya Yanuar Abd. Aziz Amin Lutfi Ni'matus Salamah Frentina Murty Sujadi Ekwan Nofa Wiratno Citra Satrya Utama Dewi Andi Kurniawan |
| author_sort | Adi Tiya Yanuar |
| collection | DOAJ |
| description | Abstract
Hydrocarbon contamination is one of the most significant environmental problems, including in the marine environment. Several methods, such as chemical and physical remediation, have been constructed to remediate pollutants but remain side effects and have a relatively high cost. Bioremediation is an alternative method for degrading contaminants due to its eco-friendliness and low cost. During bioremediation, the enzyme oxygenase is produced by bacteria to degrade pollutants. However, the oxygenation enzyme's activity and compound profile during this bioremediation process are still sparsely explored. This study aimed to analyze oxygenation enzyme activity and compound profiles during the hydrocarbon bioremediation process by bacterial consortia of Pseudomonas aeruginosa and Rhodococcus erythopolis. The materials of this research were the oxygenase enzyme activity, compound profile, and amount of BOD and COD during the bioremediation process. The results showed that the monooxygenase enzyme has activity between 0.257 and 3.859 U/mL. Meanwhile, the dioxygenase enzyme has activity between 0.579 and 5.402 U/mL. The final incubation compound profile found that Hexadecanoic acid methyl ester (C17H34O2) decreased by up to 47.66%. Moreover, BOD and COD reductions were 68.59% and 67.51%, respectively. This study concluded that oxygenation enzymes were produced to degrade pollutant compounds during the hydrocarbon bioremediation process. Further research is needed to improve the effectiveness of bioremediation by enhancing enzyme activity.
Highlight Research
• The potential of hydrocarbon-degrading bacteria (Rhodococcus erythropolis and Pseudomonas aeruginosa) for pollutant degradation in the marine environment.
• Enzyme activity of hydrocarbon-degrading bacteria and degradation profile of pollutants.
• The dynamics of BOD and COD during the bioremediation process. |
| format | Article |
| id | doaj-art-2de23312891e43b5ad28f34bc88ecb20 |
| institution | Kabale University |
| issn | 2085-5842 2528-0759 |
| language | English |
| publishDate | 2023-12-01 |
| publisher | Faculty of Fisheries and Marine Universitas Airlangga |
| record_format | Article |
| series | Jurnal Ilmiah Perikanan dan Kelautan |
| spelling | doaj-art-2de23312891e43b5ad28f34bc88ecb202025-02-03T05:30:51ZengFaculty of Fisheries and Marine Universitas AirlanggaJurnal Ilmiah Perikanan dan Kelautan2085-58422528-07592023-12-0116110611810.20473/jipk.v16i1.4552843613Oxygenase Enzyme Activity and Compound Profile in Hydrocarbon Bioremediation by Pseudomonas aeruginosa and Rhodococcus erythropolis ConsortiumAdi Tiya Yanuar0https://orcid.org/0000-0002-1476-2319Abd. Aziz Amin1Lutfi Ni'matus Salamah2Frentina Murty Sujadi3Ekwan Nofa Wiratno4Citra Satrya Utama Dewi5Andi Kurniawan6https://orcid.org/0000-0001-6301-0861Coastal and Marine Research Center, University of Brawijaya, Malang, East Java. Indonesia; Department of Marine Science, Faculty of Fisheries and Marine Sciences, University of Brawijaya, Malang, East Java. IndonesiaCoastal and Marine Research Center, University of Brawijaya, Malang, East Java. Indonesia; Department of Marine Science, Faculty of Fisheries and Marine Sciences, University of Brawijaya, Malang, East Java. IndonesiaCoastal and Marine Research Center, University of Brawijaya, Malang, East Java. Indonesia; Department of Marine Science, Faculty of Fisheries and Marine Sciences, University of Brawijaya, Malang, East Java. IndonesiaDepartment of Fisheries and Marine Resources Management, Faculty of Fisheries and Marine Sciences, University of Brawijaya, Malang, East Java. IndonesiaDepartment of Fisheries and Marine Resources Management, Faculty of Fisheries and Marine Sciences, University of Brawijaya, Malang, East Java. IndonesiaCoastal and Marine Research Center, University of Brawijaya, Malang, East Java. Indonesia; Department of Marine Science, Faculty of Fisheries and Marine Sciences, University of Brawijaya, Malang, East Java. IndonesiaCoastal and Marine Research Center, University of Brawijaya, Malang, East Java. Indonesia; Department of Fisheries and Marine Resources Management, Faculty of Fisheries and Marine Sciences, University of Brawijaya, Malang, East Java. IndonesiaAbstract Hydrocarbon contamination is one of the most significant environmental problems, including in the marine environment. Several methods, such as chemical and physical remediation, have been constructed to remediate pollutants but remain side effects and have a relatively high cost. Bioremediation is an alternative method for degrading contaminants due to its eco-friendliness and low cost. During bioremediation, the enzyme oxygenase is produced by bacteria to degrade pollutants. However, the oxygenation enzyme's activity and compound profile during this bioremediation process are still sparsely explored. This study aimed to analyze oxygenation enzyme activity and compound profiles during the hydrocarbon bioremediation process by bacterial consortia of Pseudomonas aeruginosa and Rhodococcus erythopolis. The materials of this research were the oxygenase enzyme activity, compound profile, and amount of BOD and COD during the bioremediation process. The results showed that the monooxygenase enzyme has activity between 0.257 and 3.859 U/mL. Meanwhile, the dioxygenase enzyme has activity between 0.579 and 5.402 U/mL. The final incubation compound profile found that Hexadecanoic acid methyl ester (C17H34O2) decreased by up to 47.66%. Moreover, BOD and COD reductions were 68.59% and 67.51%, respectively. This study concluded that oxygenation enzymes were produced to degrade pollutant compounds during the hydrocarbon bioremediation process. Further research is needed to improve the effectiveness of bioremediation by enhancing enzyme activity. Highlight Research • The potential of hydrocarbon-degrading bacteria (Rhodococcus erythropolis and Pseudomonas aeruginosa) for pollutant degradation in the marine environment. • Enzyme activity of hydrocarbon-degrading bacteria and degradation profile of pollutants. • The dynamics of BOD and COD during the bioremediation process.https://e-journal.unair.ac.id/JIPK/article/view/45528marine pollutionbioremediationenzymehydrocarbonmicrobial ecology |
| spellingShingle | Adi Tiya Yanuar Abd. Aziz Amin Lutfi Ni'matus Salamah Frentina Murty Sujadi Ekwan Nofa Wiratno Citra Satrya Utama Dewi Andi Kurniawan Oxygenase Enzyme Activity and Compound Profile in Hydrocarbon Bioremediation by Pseudomonas aeruginosa and Rhodococcus erythropolis Consortium Jurnal Ilmiah Perikanan dan Kelautan marine pollution bioremediation enzyme hydrocarbon microbial ecology |
| title | Oxygenase Enzyme Activity and Compound Profile in Hydrocarbon Bioremediation by Pseudomonas aeruginosa and Rhodococcus erythropolis Consortium |
| title_full | Oxygenase Enzyme Activity and Compound Profile in Hydrocarbon Bioremediation by Pseudomonas aeruginosa and Rhodococcus erythropolis Consortium |
| title_fullStr | Oxygenase Enzyme Activity and Compound Profile in Hydrocarbon Bioremediation by Pseudomonas aeruginosa and Rhodococcus erythropolis Consortium |
| title_full_unstemmed | Oxygenase Enzyme Activity and Compound Profile in Hydrocarbon Bioremediation by Pseudomonas aeruginosa and Rhodococcus erythropolis Consortium |
| title_short | Oxygenase Enzyme Activity and Compound Profile in Hydrocarbon Bioremediation by Pseudomonas aeruginosa and Rhodococcus erythropolis Consortium |
| title_sort | oxygenase enzyme activity and compound profile in hydrocarbon bioremediation by pseudomonas aeruginosa and rhodococcus erythropolis consortium |
| topic | marine pollution bioremediation enzyme hydrocarbon microbial ecology |
| url | https://e-journal.unair.ac.id/JIPK/article/view/45528 |
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