Protocol for achieving enhanced snRNA-seq data quality using Quality Clustering
Summary: Single-nucleus RNA sequencing (snRNA-seq) data analysis presents a challenge in samples that have high levels of ambient RNA contamination. Quality Clustering (QClus) removes empty and highly contaminated droplets by utilizing multiple contamination metrics. Here, we present the steps for s...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-06-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725001236 |
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