Protocol for achieving enhanced snRNA-seq data quality using Quality Clustering

Protocol for achieving enhanced snRNA-seq data quality using Quality Clustering

Summary: Single-nucleus RNA sequencing (snRNA-seq) data analysis presents a challenge in samples that have high levels of ambient RNA contamination. Quality Clustering (QClus) removes empty and highly contaminated droplets by utilizing multiple contamination metrics. Here, we present the steps for s...

Full description

Saved in:
Bibliographic Details
Main Authors: Paavo J. Tavi, Johannes Ojanen, Pia Laitinen, Suvi Linna-Kuosmanen
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:STAR Protocols
Subjects:
Bioinformatics
Single Cell
RNA-seq
Computer sciences
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725001236
Tags: Add Tag
No Tags, Be the first to tag this record!

Internet

http://www.sciencedirect.com/science/article/pii/S2666166725001236

Similar Items