Modified pegRNAs mitigate scaffold-derived prime editing by-products

Modified pegRNAs mitigate scaffold-derived prime editing by-products

Abstract Prime editors (PEs) employ reverse transcriptase (RT) to install genomic edits using a template within the prime editing guide RNA (pegRNA). RT creates a 3’ genomic flap containing the intended edit. However, reverse transcription can continue beyond the template, incorporating the pegRNA s...

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Main Authors: Panagiotis Antoniou, Louis Dacquay, Hanna Mårtensson, Katja Madeyski-Bengtson, Anna-Lena Loyd, Anna Shiriaeva, Euan Gordon, Salman Mustfa, George Thom, Pei-Pei Hsieh, Saša Šviković, Mike Firth, Nina Akrap, Marcello Maresca, Martin Peterka
Format: Article
Language:English
Published: Nature Portfolio 2025-04-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-025-58653-1
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https://doi.org/10.1038/s41467-025-58653-1

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