Biocatalytic reductive amination with CRISPR-Cas9 engineered yeast

Abstract Metabolically engineered baker’s yeast can be used to produce chiral amines through whole-cell bioconversion of prochiral ketones. This study investigates the modulation of the alanine-pyruvate metabolic node to enhance reductive amination, using the stereoselective conversion of benzylacet...

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Bibliographic Details
Main Authors: Arne Hagman, Olof Stenström, Göran Carlström, Mikael Akke, Carl Grey, Magnus Carlquist
Format: Article
Language:English
Published: Nature Portfolio 2025-05-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-025-01182-0
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