Enhancing substrate specificity of microbial transglutaminase for precise nanobody labeling

Streptomyces mobaraenesis transglutaminase (smTG) can be used for site-specific labeling of proteins with chemical groups. Here, we explored the use of modified smTG for the biosynthesis of nanobody-fluorophore conjugates (NFC). smTG catalyzes the conjugation of acyl donors containing glutamine with...

Full description

Saved in:
Bibliographic Details
Main Authors: Xinglong Wang, Kangjie Xu, Haoran Fu, Qiming Chen, Beichen Zhao, Xinyi Zhao, Jingwen Zhou
Format: Article
Language:English
Published: KeAi Communications Co., Ltd. 2025-03-01
Series:Synthetic and Systems Biotechnology
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2405805X24001339
Tags: Add Tag
No Tags, Be the first to tag this record!