Changes in Cytokines of the Bone Microenvironment during Breast Cancer Metastasis
It is commonly accepted that cancer cells interact with host cells to create a microenvironment favoring malignant colonization. The complex bone microenvironment produces an ever changing array of cytokines and growth factors. In this study, we examined levels of MCP-1, IL-6, KC, MIP-2, VEGF, MIG...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | International Journal of Breast Cancer |
| Online Access: | http://dx.doi.org/10.1155/2012/160265 |
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| _version_ | 1832564394614063104 |
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| author | Donna M. Sosnoski Venkatesh Krishnan William J. Kraemer Courtenay Dunn-Lewis Andrea M. Mastro |
| author_facet | Donna M. Sosnoski Venkatesh Krishnan William J. Kraemer Courtenay Dunn-Lewis Andrea M. Mastro |
| author_sort | Donna M. Sosnoski |
| collection | DOAJ |
| description | It is commonly accepted that cancer cells interact with host cells to create a microenvironment favoring malignant colonization. The complex bone microenvironment produces an ever changing array of cytokines and growth factors. In this study, we examined levels of MCP-1, IL-6, KC, MIP-2, VEGF, MIG, and eotaxin in femurs of athymic nude mice inoculated via intracardiac injection with MDA-MB-231GFP human metastatic breast cancer cells, MDA-MB-231BRMS1GFP, a metastasis suppressed variant, or PBS. Animals were euthanized (day 3, 11, 19, 27 after injection) to examine femoral cytokine levels at various stages of cancer cell colonization. The epiphysis contained significantly more cytokines than the diaphysis except for MIG which was similar throughout the bone. Variation among femurs was evident within all groups. By day 27, MCP-1, MIG, VEGF and eotaxin levels were significantly greater in femurs of cancer cell-inoculated mice. These pro-osteoclastic and angiogenic cytokines may manipulate the bone microenvironment to enhance cancer cell colonization. |
| format | Article |
| id | doaj-art-2b7dfa6f16774b3f9fe1735dd34accb7 |
| institution | Kabale University |
| issn | 2090-3170 2090-3189 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Breast Cancer |
| spelling | doaj-art-2b7dfa6f16774b3f9fe1735dd34accb72025-02-03T01:11:06ZengWileyInternational Journal of Breast Cancer2090-31702090-31892012-01-01201210.1155/2012/160265160265Changes in Cytokines of the Bone Microenvironment during Breast Cancer MetastasisDonna M. Sosnoski0Venkatesh Krishnan1William J. Kraemer2Courtenay Dunn-Lewis3Andrea M. Mastro4Department of Biochemistry and Molecular Biology, Penn State University, University Park, PA 16802, USADepartment of Biochemistry and Molecular Biology, Penn State University, University Park, PA 16802, USADepartment of Kinesiology, University of Connecticut, Storrs, CT 06269, USADepartment of Kinesiology, University of Connecticut, Storrs, CT 06269, USADepartment of Biochemistry and Molecular Biology, Penn State University, University Park, PA 16802, USAIt is commonly accepted that cancer cells interact with host cells to create a microenvironment favoring malignant colonization. The complex bone microenvironment produces an ever changing array of cytokines and growth factors. In this study, we examined levels of MCP-1, IL-6, KC, MIP-2, VEGF, MIG, and eotaxin in femurs of athymic nude mice inoculated via intracardiac injection with MDA-MB-231GFP human metastatic breast cancer cells, MDA-MB-231BRMS1GFP, a metastasis suppressed variant, or PBS. Animals were euthanized (day 3, 11, 19, 27 after injection) to examine femoral cytokine levels at various stages of cancer cell colonization. The epiphysis contained significantly more cytokines than the diaphysis except for MIG which was similar throughout the bone. Variation among femurs was evident within all groups. By day 27, MCP-1, MIG, VEGF and eotaxin levels were significantly greater in femurs of cancer cell-inoculated mice. These pro-osteoclastic and angiogenic cytokines may manipulate the bone microenvironment to enhance cancer cell colonization.http://dx.doi.org/10.1155/2012/160265 |
| spellingShingle | Donna M. Sosnoski Venkatesh Krishnan William J. Kraemer Courtenay Dunn-Lewis Andrea M. Mastro Changes in Cytokines of the Bone Microenvironment during Breast Cancer Metastasis International Journal of Breast Cancer |
| title | Changes in Cytokines of the Bone Microenvironment during Breast Cancer Metastasis |
| title_full | Changes in Cytokines of the Bone Microenvironment during Breast Cancer Metastasis |
| title_fullStr | Changes in Cytokines of the Bone Microenvironment during Breast Cancer Metastasis |
| title_full_unstemmed | Changes in Cytokines of the Bone Microenvironment during Breast Cancer Metastasis |
| title_short | Changes in Cytokines of the Bone Microenvironment during Breast Cancer Metastasis |
| title_sort | changes in cytokines of the bone microenvironment during breast cancer metastasis |
| url | http://dx.doi.org/10.1155/2012/160265 |
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