The Interactive Effects of Fruit Intake Frequency and Serum miR-484 Levels as Biomarkers for Incident Type 2 Diabetes in a Prospective Cohort of the Spanish Adult Population: The Di@bet.es Study
<b>Background/Objectives</b>: Although evidence suggests that miR-484 and several fruit components are involved in glucose metabolism and insulin resistance metabolic pathways, the relationship between serum miR-484 levels and fruit consumption in relation to the risk of Type 2 diabetes...
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MDPI AG
2025-01-01
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| Online Access: | https://www.mdpi.com/2227-9059/13/1/160 |
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| author | Ana Lago-Sampedro Wasima Oualla-Bachiri Cristina Maldonado-Araque Sergio Valdés Inmaculada González-Molero Viyey Doulatram-Gamgaram Elias Delgado Felipe J. Chaves Luis Castaño Alfonso Calle-Pascual Josep Franch-Nadal Gemma Rojo-Martínez Sara García-Serrano Eva García-Escobar |
| author_facet | Ana Lago-Sampedro Wasima Oualla-Bachiri Cristina Maldonado-Araque Sergio Valdés Inmaculada González-Molero Viyey Doulatram-Gamgaram Elias Delgado Felipe J. Chaves Luis Castaño Alfonso Calle-Pascual Josep Franch-Nadal Gemma Rojo-Martínez Sara García-Serrano Eva García-Escobar |
| author_sort | Ana Lago-Sampedro |
| collection | DOAJ |
| description | <b>Background/Objectives</b>: Although evidence suggests that miR-484 and several fruit components are involved in glucose metabolism and insulin resistance metabolic pathways, the relationship between serum miR-484 levels and fruit consumption in relation to the risk of Type 2 diabetes (T2DM) remains elusive. The aim of this study was to evaluate the possible association between serum miR-484 levels and fruit intake frequency with the risk of T2DM in the Spanish adult population. <b>Methods</b>: 2234 subjects from the Di@bet.es cohort study without T2DM at baseline were studied. Socio-demographic, anthropometric and clinical data were recorded, as well as responses to a questionnaire on habits, including frequency of fruit consumption (daily vs. occasional). T2DM was diagnosed at baseline and after 7.5 years of follow-up. Baseline serum miR-484 levels were measured using real-time qPCR and categorized based on the 25th percentile. Association analyses were performed using logistic regression models adjusted for potential confounders. Interaction effects were evaluated on the multiplicative and additive scales. <b>Results</b>: There was no association between miR-484 levels and fruit intake frequency. Categorized miR-484 levels and fruit consumption were inversely and independently associated with the likelihood of incident T2DM. Analysis of the interaction effect suggests the presence of both positive multiplicative and additive interactions between miR-484 categories and fruit consumption frequency. <b>Conclusions</b>: Our study demonstrates a protective effect of daily fruit intake and high miR-484 levels regarding the risk of T2DM and supports the nutritional recommendations advocating daily fruit consumption. This study also suggests that the combined effect of low miR-484 levels and occasional fruit intake may increase the risk of T2DM beyond their independent effects. |
| format | Article |
| id | doaj-art-2a8a6472f2f54c08abe48662a61edaab |
| institution | Kabale University |
| issn | 2227-9059 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Biomedicines |
| spelling | doaj-art-2a8a6472f2f54c08abe48662a61edaab2025-01-24T13:24:13ZengMDPI AGBiomedicines2227-90592025-01-0113116010.3390/biomedicines13010160The Interactive Effects of Fruit Intake Frequency and Serum miR-484 Levels as Biomarkers for Incident Type 2 Diabetes in a Prospective Cohort of the Spanish Adult Population: The Di@bet.es StudyAna Lago-Sampedro0Wasima Oualla-Bachiri1Cristina Maldonado-Araque2Sergio Valdés3Inmaculada González-Molero4Viyey Doulatram-Gamgaram5Elias Delgado6Felipe J. Chaves7Luis Castaño8Alfonso Calle-Pascual9Josep Franch-Nadal10Gemma Rojo-Martínez11Sara García-Serrano12Eva García-Escobar13Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabolicas Asociadas (CIBERDEM), Instituto de Salud Carlos III, 28029 Madrid, SpainCentro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabolicas Asociadas (CIBERDEM), Instituto de Salud Carlos III, 28029 Madrid, SpainCentro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabolicas Asociadas (CIBERDEM), Instituto de Salud Carlos III, 28029 Madrid, SpainCentro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabolicas Asociadas (CIBERDEM), Instituto de Salud Carlos III, 28029 Madrid, SpainUGC Endocrinología y Nutrición, Hospital Regional Universitario de Málaga, IBIMA Plataforma BIONAND, 29590 Malaga, SpainCentro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabolicas Asociadas (CIBERDEM), Instituto de Salud Carlos III, 28029 Madrid, SpainCentro de Investigación Biomédica en Red de Enfermedades Raras (CIBERER), Instituto de Salud Carlos III, 28029 Madrid, SpainCentro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabolicas Asociadas (CIBERDEM), Instituto de Salud Carlos III, 28029 Madrid, SpainCentro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabolicas Asociadas (CIBERDEM), Instituto de Salud Carlos III, 28029 Madrid, SpainCentro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabolicas Asociadas (CIBERDEM), Instituto de Salud Carlos III, 28029 Madrid, SpainCentro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabolicas Asociadas (CIBERDEM), Instituto de Salud Carlos III, 28029 Madrid, SpainCentro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabolicas Asociadas (CIBERDEM), Instituto de Salud Carlos III, 28029 Madrid, SpainCentro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabolicas Asociadas (CIBERDEM), Instituto de Salud Carlos III, 28029 Madrid, SpainCentro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabolicas Asociadas (CIBERDEM), Instituto de Salud Carlos III, 28029 Madrid, Spain<b>Background/Objectives</b>: Although evidence suggests that miR-484 and several fruit components are involved in glucose metabolism and insulin resistance metabolic pathways, the relationship between serum miR-484 levels and fruit consumption in relation to the risk of Type 2 diabetes (T2DM) remains elusive. The aim of this study was to evaluate the possible association between serum miR-484 levels and fruit intake frequency with the risk of T2DM in the Spanish adult population. <b>Methods</b>: 2234 subjects from the Di@bet.es cohort study without T2DM at baseline were studied. Socio-demographic, anthropometric and clinical data were recorded, as well as responses to a questionnaire on habits, including frequency of fruit consumption (daily vs. occasional). T2DM was diagnosed at baseline and after 7.5 years of follow-up. Baseline serum miR-484 levels were measured using real-time qPCR and categorized based on the 25th percentile. Association analyses were performed using logistic regression models adjusted for potential confounders. Interaction effects were evaluated on the multiplicative and additive scales. <b>Results</b>: There was no association between miR-484 levels and fruit intake frequency. Categorized miR-484 levels and fruit consumption were inversely and independently associated with the likelihood of incident T2DM. Analysis of the interaction effect suggests the presence of both positive multiplicative and additive interactions between miR-484 categories and fruit consumption frequency. <b>Conclusions</b>: Our study demonstrates a protective effect of daily fruit intake and high miR-484 levels regarding the risk of T2DM and supports the nutritional recommendations advocating daily fruit consumption. This study also suggests that the combined effect of low miR-484 levels and occasional fruit intake may increase the risk of T2DM beyond their independent effects.https://www.mdpi.com/2227-9059/13/1/160type 2 diabetes mellitusincidencebiomarkersmiR-484fruit intakeinteraction |
| spellingShingle | Ana Lago-Sampedro Wasima Oualla-Bachiri Cristina Maldonado-Araque Sergio Valdés Inmaculada González-Molero Viyey Doulatram-Gamgaram Elias Delgado Felipe J. Chaves Luis Castaño Alfonso Calle-Pascual Josep Franch-Nadal Gemma Rojo-Martínez Sara García-Serrano Eva García-Escobar The Interactive Effects of Fruit Intake Frequency and Serum miR-484 Levels as Biomarkers for Incident Type 2 Diabetes in a Prospective Cohort of the Spanish Adult Population: The Di@bet.es Study Biomedicines type 2 diabetes mellitus incidence biomarkers miR-484 fruit intake interaction |
| title | The Interactive Effects of Fruit Intake Frequency and Serum miR-484 Levels as Biomarkers for Incident Type 2 Diabetes in a Prospective Cohort of the Spanish Adult Population: The Di@bet.es Study |
| title_full | The Interactive Effects of Fruit Intake Frequency and Serum miR-484 Levels as Biomarkers for Incident Type 2 Diabetes in a Prospective Cohort of the Spanish Adult Population: The Di@bet.es Study |
| title_fullStr | The Interactive Effects of Fruit Intake Frequency and Serum miR-484 Levels as Biomarkers for Incident Type 2 Diabetes in a Prospective Cohort of the Spanish Adult Population: The Di@bet.es Study |
| title_full_unstemmed | The Interactive Effects of Fruit Intake Frequency and Serum miR-484 Levels as Biomarkers for Incident Type 2 Diabetes in a Prospective Cohort of the Spanish Adult Population: The Di@bet.es Study |
| title_short | The Interactive Effects of Fruit Intake Frequency and Serum miR-484 Levels as Biomarkers for Incident Type 2 Diabetes in a Prospective Cohort of the Spanish Adult Population: The Di@bet.es Study |
| title_sort | interactive effects of fruit intake frequency and serum mir 484 levels as biomarkers for incident type 2 diabetes in a prospective cohort of the spanish adult population the di bet es study |
| topic | type 2 diabetes mellitus incidence biomarkers miR-484 fruit intake interaction |
| url | https://www.mdpi.com/2227-9059/13/1/160 |
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