Spatially resolved gene expression profiling of tumor microenvironment reveals key steps of lung adenocarcinoma development
Abstract The interaction of tumor cells and their microenvironment is thought to be a key factor in tumor development. We present spatial RNA profiles obtained from 30 lung adenocarcinoma patients at the non-invasive and later invasive stages. We use spatial transcriptome sequencing data in conjunct...
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| Format: | Article |
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Nature Portfolio
2024-12-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-024-54671-7 |
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| author | Yuma Takano Jun Suzuki Kotaro Nomura Gento Fujii Junko Zenkoh Hitomi Kawai Yuta Kuze Yukie Kashima Satoi Nagasawa Yuka Nakamura Motohiro Kojima Katsuya Tsuchihara Masahide Seki Akinori Kanai Daisuke Matsubara Takashi Kohno Masayuki Noguchi Akihiro Nakaya Masahiro Tsuboi Genichiro Ishii Yutaka Suzuki Ayako Suzuki |
| author_facet | Yuma Takano Jun Suzuki Kotaro Nomura Gento Fujii Junko Zenkoh Hitomi Kawai Yuta Kuze Yukie Kashima Satoi Nagasawa Yuka Nakamura Motohiro Kojima Katsuya Tsuchihara Masahide Seki Akinori Kanai Daisuke Matsubara Takashi Kohno Masayuki Noguchi Akihiro Nakaya Masahiro Tsuboi Genichiro Ishii Yutaka Suzuki Ayako Suzuki |
| author_sort | Yuma Takano |
| collection | DOAJ |
| description | Abstract The interaction of tumor cells and their microenvironment is thought to be a key factor in tumor development. We present spatial RNA profiles obtained from 30 lung adenocarcinoma patients at the non-invasive and later invasive stages. We use spatial transcriptome sequencing data in conjunction with in situ RNA profiling to conduct higher resolution analyses. The detailed examination of each case, as well as the subsequent computational analyses based on the observed diverse profiles, reveals that significant changes in the phenotypic appearances of tumor cells are frequently associated with changes in immune cell features. The phenomenon coincides with the induction of a series of cellular expression programs that enable tumor cells to transform and break through the immune cell barrier, allowing them to progress further. The study shows how lung tumors develop through interaction in their microenvironments. |
| format | Article |
| id | doaj-art-2a00bf58cd224c8a8a2cb08537990a4a |
| institution | OA Journals |
| issn | 2041-1723 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-2a00bf58cd224c8a8a2cb08537990a4a2025-08-20T02:20:45ZengNature PortfolioNature Communications2041-17232024-12-0115111710.1038/s41467-024-54671-7Spatially resolved gene expression profiling of tumor microenvironment reveals key steps of lung adenocarcinoma developmentYuma Takano0Jun Suzuki1Kotaro Nomura2Gento Fujii3Junko Zenkoh4Hitomi Kawai5Yuta Kuze6Yukie Kashima7Satoi Nagasawa8Yuka Nakamura9Motohiro Kojima10Katsuya Tsuchihara11Masahide Seki12Akinori Kanai13Daisuke Matsubara14Takashi Kohno15Masayuki Noguchi16Akihiro Nakaya17Masahiro Tsuboi18Genichiro Ishii19Yutaka Suzuki20Ayako Suzuki21Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, The University of TokyoDepartment of Thoracic Surgery, National Cancer Center Hospital EastDepartment of Thoracic Surgery, National Cancer Center Hospital EastDepartment of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, The University of TokyoDepartment of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, The University of TokyoDepartment of Diagnostic Pathology, Faculty of Medicine, University of TsukubaDepartment of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, The University of TokyoDepartment of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, The University of TokyoDepartment of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, The University of TokyoDivision of Pathology, Exploratory Oncology Research and Clinical Trial Center, National Cancer CenterDivision of Pathology, Exploratory Oncology Research and Clinical Trial Center, National Cancer CenterDivision of Translational Informatics, Exploratory Oncology Research and Clinical Trial Center, National Cancer CenterDepartment of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, The University of TokyoDepartment of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, The University of TokyoDepartment of Diagnostic Pathology, Faculty of Medicine, University of TsukubaDivision of Genome Biology, National Cancer Center Research InstituteDepartment of Diagnostic Pathology, Faculty of Medicine, University of TsukubaDepartment of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, The University of TokyoDepartment of Thoracic Surgery, National Cancer Center Hospital EastDivision of Pathology, Exploratory Oncology Research and Clinical Trial Center, National Cancer CenterDepartment of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, The University of TokyoDepartment of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, The University of TokyoAbstract The interaction of tumor cells and their microenvironment is thought to be a key factor in tumor development. We present spatial RNA profiles obtained from 30 lung adenocarcinoma patients at the non-invasive and later invasive stages. We use spatial transcriptome sequencing data in conjunction with in situ RNA profiling to conduct higher resolution analyses. The detailed examination of each case, as well as the subsequent computational analyses based on the observed diverse profiles, reveals that significant changes in the phenotypic appearances of tumor cells are frequently associated with changes in immune cell features. The phenomenon coincides with the induction of a series of cellular expression programs that enable tumor cells to transform and break through the immune cell barrier, allowing them to progress further. The study shows how lung tumors develop through interaction in their microenvironments.https://doi.org/10.1038/s41467-024-54671-7 |
| spellingShingle | Yuma Takano Jun Suzuki Kotaro Nomura Gento Fujii Junko Zenkoh Hitomi Kawai Yuta Kuze Yukie Kashima Satoi Nagasawa Yuka Nakamura Motohiro Kojima Katsuya Tsuchihara Masahide Seki Akinori Kanai Daisuke Matsubara Takashi Kohno Masayuki Noguchi Akihiro Nakaya Masahiro Tsuboi Genichiro Ishii Yutaka Suzuki Ayako Suzuki Spatially resolved gene expression profiling of tumor microenvironment reveals key steps of lung adenocarcinoma development Nature Communications |
| title | Spatially resolved gene expression profiling of tumor microenvironment reveals key steps of lung adenocarcinoma development |
| title_full | Spatially resolved gene expression profiling of tumor microenvironment reveals key steps of lung adenocarcinoma development |
| title_fullStr | Spatially resolved gene expression profiling of tumor microenvironment reveals key steps of lung adenocarcinoma development |
| title_full_unstemmed | Spatially resolved gene expression profiling of tumor microenvironment reveals key steps of lung adenocarcinoma development |
| title_short | Spatially resolved gene expression profiling of tumor microenvironment reveals key steps of lung adenocarcinoma development |
| title_sort | spatially resolved gene expression profiling of tumor microenvironment reveals key steps of lung adenocarcinoma development |
| url | https://doi.org/10.1038/s41467-024-54671-7 |
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