黑曲霉C112脂肪酶基因的克隆及异源表达优化A novel lipase gene of Aspergillus niger C112 cloning, and heterologous expression optimization

黑曲霉C112脂肪酶基因的克隆及异源表达优化A novel lipase gene of Aspergillus niger C112 cloning, and heterologous expression optimization

旨在为脂肪酶的改性及其工业化应用提供参考，基于黑曲霉C112菌株通过PCR扩增得到脂肪酶基因，对该基因进行了生物信息学分析，并运用基因工程技术构建了毕赤酵母GS115重组菌，对该重组菌诱导产生的脂肪酶进行了纯化，分析了该脂肪酶的酶学性质，并通过单因素实验和响应面实验对该重组菌产酶条件进行了优化。结果表明：该脂肪酶基因片段长度为894 bp，理论分子质量为31.79 kDa，其氨基酸序列中存在一个新的五肽基序RHSLG；该脂肪酶最适反应温度和pH分别为40 ℃和4，具有良好的热稳定性和pH稳定性；重组菌最佳产酶条件为YNB添加量10.50 mL（100 mL BMMY培养基中）、接种量4.3%、...

Full description

Saved in:

Bibliographic Details
Main Author:	徐鹏钧1,2，曾柏全1,2，肖志红3，李美群1,3，张轩1,3，李培旺3，赵茜彤1，颜丽安1 XU Pengjun1,2, ZENG Baiquan1,2, XIAO Zhihong3, LI Meiqun1,3, ZHANG Xuan1,3, LI Peiwang3, ZHAO Xitong1, YAN Li′an1
Format:	Article
Language:	English
Published:	中粮工科（西安）国际工程有限公司 2025-04-01
Series:	Zhongguo youzhi
Subjects:	黑曲霉c112；脂肪酶；毕赤酵母；生物信息学；克隆 aspergillus niger c112; lipase; pichia pastoris; bioinformatics; cloning
Online Access:	http://tg.chinaoils.cn/zgyz/ch/reader/create_pdf.aspx?file_no=20250412&flag=1
Tags:	Add Tag No Tags, Be the first to tag this record!

Similar Items

SPIRIT 2025声明解读：开放科学 Interpretation of the SPIRIT 2025 Statement: Open Science
by: 李辉1，2，谢雪微1，3 (LI Hui1,2, XIE Xuewei1,3)
Published: (2025-05-01)

CONSORT 2025声明解读：患者及公众参与说明 Interpretation of the CONSORT 2025 Statement: Explanation of Patient and Public Involvement
by: 李辉1，2，谢雪微1，3 (LI Hui1,2, XIE Xuewei1,3 )
Published: (2025-05-01)

SPIRIT 2025声明：随机试验方案指南更新版 SPIRIT 2025 Statement: Updated Guideline for Protocols of Randomised Trials
by: 译者：李辉1，2，谢雪微1，3 (Translator: LI Hui1,2, XIE Xuewei1,3 )
Published: (2025-05-01)

CONSORT 2025声明：随机试验报告指南更新版 CONSORT 2025 Statement: Updated Guideline for Reporting Randomised Trials
by: 译者：李辉1，2，谢雪微1，3 (Translator: LI Hui1,2, XIE Xuewei1,3 )
Published: (2025-05-01)

Ethnobotanical survey and research on bsang plants in Xizang Autonomous Region
by: ZHANG Xiong1,2, FENG Haowen1,3, WANG Jin1,3, LIN Lei1,3, WANG Yuhua1*
Published: (2024-10-01)

美国心脏学会/美国卒中学会卒中一级预防指南：十年磨一剑 The 2024 Guideline for the Primary Prevention of Stroke: Ten Years of Hard and Persistent Effort
by: 郑华光1，2，3，李昊1，3，王拥军1，3 (ZHENG Huaguang1,2,3, LI Hao1,3, WANG Yongjun1,3 )
Published: (2024-12-01)

基于SSR分子标记的油棕遗传多样性及群体遗传结构分析Analysis of genetic diversity and population genetic structure in oil palm based on SSR markers
by: 刘加能1,2，程秋如1,2，潘登浪1，李炜芳1，彭小芸1,2，周若茹1,2，周丽霞3，曾宪海1,3，刘子凡1,2 LIU Jianeng1,2，CHENG Qiuru1,2，PAN Denglang1，LI Weifang1，PENG Xiaoyun1,2， ZHOU Ruoru1,2，ZHOU Lixia3，ZENG Xianhai1,3，LIU Zifan1,2
Published: (2025-04-01)

Chemical constituents of the roots and rhizomata of Torricellia angulata var. intermedia
by: ZHU Banghui1,3, NIE Yushan1,3, LI Xue1,3, LIU Chunhua1, LI Yueting2, MA Xue1 , LI Yongjun1,2,3*
Published: (2024-12-01)

基于脑影像及临床特征的机器学习模型预测缺血性卒中后心房颤动 A Machine Learning Model Based on Brain Imaging and Clinical Features for Predicting Atrial Fibrillation Detected after Stroke
by: 张栗源1，刘涛2，姜勇1，李子孝1，3，王拥军1，3，杨晓萌1，3(ZHANG Liyuan1, LIU Tao2, JIANG Yong1, LI Zixiao1,3, WANG Yongjun1,3, YANG Xiaomeng1,3 )
Published: (2025-04-01)

芝麻素对调和油煎炸稳定性的影响Effect of sesamin on frying stability of blend oil
by: 张天宇1，魏学鼎1，王澍2，何东平1,2,3，雷芬芬1,3，周力1,3 ZHANG Tianyu1, WEI Xueding1, WANG Shu2, HE Dongping1,2,3, LEI Fenfen1,3, ZHOU Li1,3
Published: (2025-07-01)

椰子油的化学组成及功效研究进展Research progress on chemical composition and efficacy of coconut oil
by: 呙兴阳1,2，殷娇娇1,2，王澍3,4，黄何1,2，高盼1,2,3，潘保辉1,2，钟武1,2,3，胡传荣1,2，何东平1,2,3，常超1,2 GUO Xingyang1,2, YIN Jiaojiao1,2, WANG Shu3,4, HUANG He1,2, GAO Pan1,2,3, PAN Baohui1,2, ZHONG Wu1,2,3, HU Chuanrong1,2, HE Dongping1,2,3, CHANG Chao1,2
Published: (2025-06-01)

动态脑血流自动调节功能检查对急性缺血性卒中血管内血栓切除术后完全再通患者不良预后的预测价值 The Predictive Value of Dynamic Cerebral Autoregulation for Poor Prognosis in Acute Ischemic Stroke Patients with Complete Recanalization after Endovascular Thrombectomy
by: 刘然1，2，王平平3，陈虹秀1，2，李娜1，2，周福波1，2，赵文博3，马青峰3，邢英琦1，2 (LIU Ran1,2, WANG Pingping3, CHEN Hongxiu1,2, LI Na1,2, ZHOU Fubo1,2, ZHAO Wenbo3, MA Qingfeng3, XING Yingqi1,2 )
Published: (2025-03-01)

脑血管病的多组学研究 Multi-omics Research in Cerebrovascular Disease
by: 张杰1，2，李昊1，2，3，程丝1，2，3 （ZHANG Jie1,2, LI Hao1,2,3, CHENG Si1,2,3 ）
Published: (2025-06-01)

超高效液相色谱-三重四极杆串联质谱法同时测定植物油中4种维生素E异构体Simultaneous determination of four vitamin E isomers in vegetable oil by ultra-high performance liquid chromatography- triple quadrupole tandem mass spectrometry
by: 李彩玲1，张见1,2，刘元法1,3，王晓超1，梅东旭1 LI Cailing1, ZHANG Jian1,2, LIU Yuanfa1,3, WANG Xiaochao1, MEI Dongxu1
Published: (2025-01-01)

1990—2021年中国归因于高LDL-C的卒中死亡负担变化趋势及预测分析 Trends and Predictive Analysis of the Burden of Stroke Deaths Attributable to High LDL-C in China from 1990 to 2021
by: 张永庆1，2，李娜1，2，高伊丽1，2，秦佳文1，2，俞海萍1，2，赵婷婷3 (ZHANG Yongqing1,2, LI Na1,2, GAO Yili1,2, QIN Jiawen1,2, YU Haiping1,2, ZHAO Tingting3 )
Published: (2025-04-01)

硫苷降解菌筛选及改良菜籽粕营养特性的研究Screening of glucosinolates-degrading bacteria and their role in improving the nutrition characteristics of rapeseed meal
by: 张寒1,2，韩梅1,2，郑竟成1,2，罗质1,2，雷芬芬1,2,3，陈东1,2，周力1,2，何东平1,2,3，刘曙4，洪坤强1,2,3 ZHANG Han1,2, HAN Mei1,2, ZHENG Jingcheng1,2, LUO Zhi1,2, LEI Fenfen1,2,3, CHEN Dong1,2, ZHOU Li1,2, HE Dongping1,2,3, LIU Shu4, HONG Kunqiang1,2,3
Published: (2025-03-01)

全基因组关联分析标准化流程的构建与扩展应用(Development and Extended Applications of Standardized Processes for Genome-Wide Association Studies)
by: 许喆1，2，石延枫1，2，张杰1，2，姜明慧1，2，刘阳1，2，李昊1，2，3，廖晓凌4，程丝1，2，3(XU Zhe1,2, SHI Yanfeng1,2, ZHANG Jie1,2, JIANG Minghui1,2, LIU Yang1,2, LI Hao1,2,3, LIAO Xiaoling4, CHENG Si1,2,3)
Published: (2025-06-01)

经颅多普勒卧立位试验在脑血管病中的应用进展 Application Progress of the Recumbent-Upright Test Using Transcranial Doppler in Cerebrovascular Diseases
by: 王静祎1，2，李华1，2，曹黎明3，4 (WANG Jingyi1,2, LI Hua1,2, CAO Liming3,4 )
Published: (2024-09-01)

Floristic characteristics of vascular plants in Qilian Mountain region, China
by: LI Jianan1,2,3, WANG Bo1,2,3, ZHAO Shuo1,2,3, ZHANG Famei1,2,3, ZHOU Guoying1,2 *
Published: (2024-08-01)

Diversity and antibacterial activity of culturable bacteria from rhizosphere soil in seagrass bed halophytes
by: CHEN Wang'an1,2,3, LI Mi1,2, LI Fangting1,2, GAO Chenghai1,2*
Published: (2024-08-01)

Response of seedling community to season and topography in mid-mountain moist evergreen broad-leaved forest in Ailaoshan, Yunnan
by: SHEN Jinfeng1,2, SONG Xiaoyang1*, WEN Handong1, LU Zhiyun1,3, YANG Jie1, CAO Min1
Published: (2024-08-01)

冷冻结晶-高效液相色谱法定量分析食用油中4种生育酚Quantitative analysis of four tocopherols in edible oils by freezing crystallization-high performance liquid chromatography method
by: 裘董琦1,2，赵引弟1,2，裴栋2,3，黄新异1,2，邸多隆1,2 QIU Dongqi1,2, ZHAO Yindi1,2, PEI Dong2,3, HUANG Xinyi1,2, DI Duolong1,2
Published: (2025-08-01)

Variations of microbial communities and enzyme activities in rhizosphere and non-rhizosphere soils of aged Loropetalum chinense forests in karst rocky mountains during dry and rainy seasons
by: WANG Yanan1,2, MA Jiangming1,2*, LIANG Yueming3, YANG Hao1,2
Published: (2024-10-01)

Study on the seed plant flora of Qinghai Province, China
by: WU Yuhu1,2, PANG Zhe1,2*, LI Congjia3, ZHANG Zelin3
Published: (2024-08-01)

蛋黄脂质粉末油脂制备工艺优化及性质研究Optimization of preparation process and properties of egg yolk lipids powdered oil
by: 蔡芸丹1，2，饶胜其3，顾璐萍1，2，常翠华1，2，李俊华1，2，杨严俊1，2，苏宇杰1，2 CAI Yundan1,2，RAO Shengqi3，GU Luping1,2，CHANG Cuihua1,2， LI Junhua1,2, YANG Yanjun1,2, SU Yujie1,2
Published: (2025-05-01)

pH对玉米蛋白水解物功能特性的影响及其抗氧化活性分析Effect of pH on functional properties of corn protein hydrolysates and its antioxidant activity analysis
by: 李冠龙1,2，赵玉昊1,2，刘晓兰1,2，景言1，郑喜群2,3 LI Guanlong1,2, ZHAO Yuhao1,2, LIU Xiaolan1,2, JING Yan1, ZHENG Xiqun2,3
Published: (2025-05-01)

稀奶油乳液储藏期间稳定性变化对其搅打性能的影响 Effects of changes in stability on whipping performance of whipping cream emulsion during storage
by: 谢朋凯1，崔利敏2，那尔布兰·阿依本1，金俊1,3，金青哲1，王兴国1,3 XIE Pengkai1, CUI Limin2, AYIBEN Naerbulan1, JIN Jun1,3, JIN Qingzhe1, WANG Xingguo1,3
Published: (2025-06-01)

Spatial distribution of global world natural heritage sites and suggestions for screening potential areas
by: LIU Mao1,2, XU Xue1,2, LUO Ya1,2*, YANG Shengtian3,4, SHI Chunmao1,2, ZHAO Shuang1,2, LIAO Mengyao1,2
Published: (2024-08-01)

纳米材料在缺血性卒中诊疗中的研究进展及挑战 Research Progress and Challenges of Nanomaterials in the Diagnosis and Treatment of Ischemic Stroke
by: 张丽苹1*，曹黎明2*，肖楠1，3，廖雨琦1，池枫1，余艳妮1，3，任力杰1（*第一作者） (ZHANG Liping1*, CAO Liming2*, XIAO Nan1,3, LIAO Yuqi1, CHI Feng1, YU Yanni1,3, REN Lijie1 (*contributed equally). )
Published: (2024-09-01)

阴离子多糖的结构特性及其在高水分组织化植物蛋白中应用的研究进展Research progress on the structural characteristics of anionic polysaccharides and their application in highmoisture textured vegetable protein
by: 安红周1,2，任静文1,2，郭益廷2，李盘欣3，黄亚男3 AN Hongzhou1,2, REN Jingwen1,2, GUO Yiting2, LI Panxin3, HUANG Yanan3
Published: (2025-04-01)

高蛋白脱酚棉籽粕制取工艺研究Preparation of high protein degossypoled cottonseed meal
by: 王如南1,2，张扬3，韩文杰2,3 WANG Runan1,2, ZHANG Yang3, HAN Wenjie2,3
Published: (2024-12-01)

Floristic geography of woody plants in the big plot of Gulinqing karst forest in Yunnan Province
by: LIU Qun1,2, CHEN Wenhong1, HUANG Hong1,3, YANG Chong1, FAN Changli1, ZHANG Jinguo4, SHUI Yumin1*
Published: (2024-10-01)

3种保健功能性油脂中氯丙醇酯和缩水甘油酯的污染调查与健康风险评估Contamination investigation and health risk assessment of chloropropanol esters and glycidyl esters in three health functional oils
by: 李益1,2，傅武胜1,2,3,4，倪蕾2，陈锦雄2,3，蔡家铭2，唐翠英1,2 LI Yi1,2, FU Wusheng1,2,3, 4, NI Lei2, CHEN Jinxiong2,3, CAI Jiaming2, TANG Cuiying1,2
Published: (2025-04-01)

油茶籽淀粉的结构与体外模拟消化特性研Structure and digestion property in vitro of Camellia oleifera starch
by: 向敏1，韩小苗1,2，吴苏喜1,2，李万元2，彭邵锋3 XIANG Min1,HAN Xiaomiao1,2,WU Suxi1,2， LI Wanyuan2,PENG Shaofeng3"
Published: (2025-01-01)

基于GC-IMS技术分析新疆不同品种核桃油挥发性成分的差异Differences of volatile components of different varieties of walnut oils in Xinjiang using gas chromatography-ion mobility spectrometry
by: 孟新涛1,2，许铭强1,2，张婷1,2，古丽米热·祖努纳1,2，牛逍瞳1,2，郭金宝3，刘国庆4，马燕1,2 MENG Xintao1,2, XU Mingqiang1,2, ZHANG Ting1,2, GULIMIRE Zununa1,2, NIU Xiaotong1,2, GUO Jinbao3, LIU Guoqing4, MA Yan1,2
Published: (2025-03-01)

残余代谢危险因素与青年卒中 Residual Metabolic Risk Factors and Stroke in Young Adults
by: 钱宇彤1，2，张书凡1，2，韩振翔3，吴维诚4，韩翔1，2 (QIAN Yutong1,2, ZHANG Shufan1,2, HAN Zhenxiang3, WU Weicheng4, HAN Xiang1,2 )
Published: (2024-12-01)

食用植物油中邻苯二甲酸酯的研究进展Research progress on phthalate esters in edible vegetable oils
by: 江仪婷1，魏骊霏1，侯旭杰1,2，赵文革3，蒲云峰1,2 JIANG Yiting1, WEI Lifei1, HOU Xujie1,2, ZHAO Wenge3, PU Yunfeng1,2
Published: (2024-12-01)

火麻蛋白肽体内降血压功效研究Hypotensive effect in vivo of hemp protein peptides
by: 卫萍1,2，刘国明2,3，韦珍1，邓中霖1 WEI Ping1,2, LIU Guoming2,3, WEI Zhen1, DENG Zhonglin1
Published: (2025-04-01)

没食子酸丙酯对牛油氧化稳定性及抗氧化能力的影响Effect of propyl gallate on oxidant stability and antioxidant capacity of tallow
by: 张远鹏1，高盼1,2，王澍3，殷娇娇1，钟武1,2，张燕鹏1 ZHANG Yuanpeng1, GAO Pan1,2, WANG Shu3, YIN Jiaojiao1, ZHONG Wu1,2, ZHANG Yanpeng1
Published: (2025-08-01)

赤大袋鼠馆气载需氧菌分离鉴定及致病性分析
by: 李俊娴 1 #李敏捷 2, et al.
Published: (2019-01-01)