The effect of NF-kB and MAPK mediated Proinflammatory microenvironment on renal aging and amyloid deposition in elder rats
Abstract Low-grade inflammation associated with the aging process exerts functional and morphological effects on multiple organs. The kidney is an organ that is severely affected by the aging process. Our study aimed to determine the relationship between histopathological and immunohistochemical cha...
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Nature Portfolio
2025-08-01
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| Online Access: | https://doi.org/10.1038/s41598-025-14559-y |
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| author | Mete Keçeci Cenk Murat Özer Esra Babaoğlu Furkan Bodur Ali Can Önal Ayşe Zeynep Yılmazer Kayatekin Osman Cengil Ali Serdar Karataş |
| author_facet | Mete Keçeci Cenk Murat Özer Esra Babaoğlu Furkan Bodur Ali Can Önal Ayşe Zeynep Yılmazer Kayatekin Osman Cengil Ali Serdar Karataş |
| author_sort | Mete Keçeci |
| collection | DOAJ |
| description | Abstract Low-grade inflammation associated with the aging process exerts functional and morphological effects on multiple organs. The kidney is an organ that is severely affected by the aging process. Our study aimed to determine the relationship between histopathological and immunohistochemical changes and age-related amyloid accumulation in rat kidney tissue. Wistar albino rats were divided into two groups: young adult and elder (n = 8, 24 and 104 weeks old, respectively). H&E, PAS, and Congo red staining and immunohistochemical staining for TNF-α, IL-6, occludin, GRP78, and mitogen-activated protein kinase (MAPK) were performed on the kidney tissues of the subjects. A significant difference was found between the groups in terms of glomerulus diameter/Bowman’s capsule diameter, percentage of sclerotic glomeruli, epithelial desquamation, loss of brush border, casts, tubular dilatation, cellular debris, and the presence of apoptotic cells. TNF-α, IL-6, GRP78, and MAPK protein levels were shown to increase significantly in aged rat kidneys, whereas occludin levels decreased significantly. No amyloid accumulation was found in the kidney, heart, skin, and small intestine tissues of either group. The results of our study suggest that the proinflammatory microenvironment required for AA amyloid deposition in systemic amyloidosis induced by low-grade inflammation in old age cannot be generated by the action of NF-κB, MAPK, and MAPK-activated JNK and AP-1 transcription factors alone. |
| format | Article |
| id | doaj-art-27cb1f95602847cf91036de7a6af0396 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-27cb1f95602847cf91036de7a6af03962025-08-24T11:18:50ZengNature PortfolioScientific Reports2045-23222025-08-0115111410.1038/s41598-025-14559-yThe effect of NF-kB and MAPK mediated Proinflammatory microenvironment on renal aging and amyloid deposition in elder ratsMete Keçeci0Cenk Murat Özer1Esra Babaoğlu2Furkan Bodur3Ali Can Önal4Ayşe Zeynep Yılmazer Kayatekin5Osman Cengil6Ali Serdar Karataş7Faculty of Medicine, Department of Histology and Embryology, Zonguldak Bülent Ecevit UniversityFaculty of Medicine, Department of Anatomy, Tekirdağ Namık Kemal UniversityFaculty of Medicine, Department of Anatomy, Zonguldak Bülent Ecevit UniversityFaculty of Medicine, Department of Anatomy, Zonguldak Bülent Ecevit UniversityFaculty of Medicine, Department of Pathology, Zonguldak Bülent Ecevit UniversityFaculty of Medicine, Department of Anatomy, Tekirdağ Namık Kemal UniversityFaculty of Medicine, Department of Histology and Embryology, Zonguldak Bülent Ecevit UniversityFaculty of Medicine, Department of Histology and Embryology, Zonguldak Bülent Ecevit UniversityAbstract Low-grade inflammation associated with the aging process exerts functional and morphological effects on multiple organs. The kidney is an organ that is severely affected by the aging process. Our study aimed to determine the relationship between histopathological and immunohistochemical changes and age-related amyloid accumulation in rat kidney tissue. Wistar albino rats were divided into two groups: young adult and elder (n = 8, 24 and 104 weeks old, respectively). H&E, PAS, and Congo red staining and immunohistochemical staining for TNF-α, IL-6, occludin, GRP78, and mitogen-activated protein kinase (MAPK) were performed on the kidney tissues of the subjects. A significant difference was found between the groups in terms of glomerulus diameter/Bowman’s capsule diameter, percentage of sclerotic glomeruli, epithelial desquamation, loss of brush border, casts, tubular dilatation, cellular debris, and the presence of apoptotic cells. TNF-α, IL-6, GRP78, and MAPK protein levels were shown to increase significantly in aged rat kidneys, whereas occludin levels decreased significantly. No amyloid accumulation was found in the kidney, heart, skin, and small intestine tissues of either group. The results of our study suggest that the proinflammatory microenvironment required for AA amyloid deposition in systemic amyloidosis induced by low-grade inflammation in old age cannot be generated by the action of NF-κB, MAPK, and MAPK-activated JNK and AP-1 transcription factors alone.https://doi.org/10.1038/s41598-025-14559-yAgingInflammationNF-κBMAPKTNF-αIL-6 |
| spellingShingle | Mete Keçeci Cenk Murat Özer Esra Babaoğlu Furkan Bodur Ali Can Önal Ayşe Zeynep Yılmazer Kayatekin Osman Cengil Ali Serdar Karataş The effect of NF-kB and MAPK mediated Proinflammatory microenvironment on renal aging and amyloid deposition in elder rats Scientific Reports Aging Inflammation NF-κB MAPK TNF-α IL-6 |
| title | The effect of NF-kB and MAPK mediated Proinflammatory microenvironment on renal aging and amyloid deposition in elder rats |
| title_full | The effect of NF-kB and MAPK mediated Proinflammatory microenvironment on renal aging and amyloid deposition in elder rats |
| title_fullStr | The effect of NF-kB and MAPK mediated Proinflammatory microenvironment on renal aging and amyloid deposition in elder rats |
| title_full_unstemmed | The effect of NF-kB and MAPK mediated Proinflammatory microenvironment on renal aging and amyloid deposition in elder rats |
| title_short | The effect of NF-kB and MAPK mediated Proinflammatory microenvironment on renal aging and amyloid deposition in elder rats |
| title_sort | effect of nf kb and mapk mediated proinflammatory microenvironment on renal aging and amyloid deposition in elder rats |
| topic | Aging Inflammation NF-κB MAPK TNF-α IL-6 |
| url | https://doi.org/10.1038/s41598-025-14559-y |
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