Preparation and Controlled Release Properties of Probiotic Hydrogel Carrier Based on Citrus Cellulose

To enhance the survival rate and release rate of probiotics in gastrointestinal fluids, hydrogels were prepared using citrus cellulose and sodium carboxymethylcellulose as embedding materials, with citric acid as the cross-linking agent. Lactiplantibacillus plantarum B5 was adsorbed and embedded in...

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Main Authors: Shaoyang WANG, Ziyuan LI, Lina QIN, Xinyi CHEN, Liqi WANG, Tiemin JIANG, Haiyun LI
Format: Article
Language:zho
Published: The editorial department of Science and Technology of Food Industry 2025-03-01
Series:Shipin gongye ke-ji
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Online Access:http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024040044
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author Shaoyang WANG
Ziyuan LI
Lina QIN
Xinyi CHEN
Liqi WANG
Tiemin JIANG
Haiyun LI
author_facet Shaoyang WANG
Ziyuan LI
Lina QIN
Xinyi CHEN
Liqi WANG
Tiemin JIANG
Haiyun LI
author_sort Shaoyang WANG
collection DOAJ
description To enhance the survival rate and release rate of probiotics in gastrointestinal fluids, hydrogels were prepared using citrus cellulose and sodium carboxymethylcellulose as embedding materials, with citric acid as the cross-linking agent. Lactiplantibacillus plantarum B5 was adsorbed and embedded in the hydrogels to evaluate the embedding rate, controlled release performance, bile salt tolerance, and storage stability. Results demonstrated that hydrogels with varying ratios exhibited favorable embedding effects, with an embedding rate exceeding 79%. The hydrogel with a citrus cellulose to sodium carboxymethylcellulose ratio of 1:4 showed optimal controlled release performance, achieving a release rate of 89.25% in simulated intestinal fluid. The bacteriophage-carrying hydrogel significantly enhanced the survival rate of L. plantarum B5 in bile salt solution, reaching 87.09%. The hydrogel maintained a storage stability of 30 days at 4 ℃, with the survival rate of embedded L. plantarum B5 exceeding 60% after 30 days, particularly reaching 70.66% with the 1:4 ratio of citrus cellulose to sodium carboxymethylcellulose, and maintaining viable bacteria levels above 7 lg CFU/mL. Studies have shown that citrus cellulose-sodium carboxymethylcellulose hydrogels are effective in reducing the exposure of probiotics to unfavorable factors in the external environment, thus significantly improving their survival in various environments.
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publisher The editorial department of Science and Technology of Food Industry
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spelling doaj-art-27ae90ea02074de9b9b2de0bfee766ff2025-08-20T03:00:55ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062025-03-0146515315910.13386/j.issn1002-0306.20240400442024040044-5Preparation and Controlled Release Properties of Probiotic Hydrogel Carrier Based on Citrus CelluloseShaoyang WANG0Ziyuan LI1Lina QIN2Xinyi CHEN3Liqi WANG4Tiemin JIANG5Haiyun LI6College of Chemistry and Biological Engineering, Guilin University of Technology, Guilin 541004, ChinaCollege of Chemistry and Biological Engineering, Guilin University of Technology, Guilin 541004, ChinaCollege of Chemistry and Biological Engineering, Guilin University of Technology, Guilin 541004, ChinaCollege of Chemistry and Biological Engineering, Guilin University of Technology, Guilin 541004, ChinaCollege of Chemistry and Biological Engineering, Guilin University of Technology, Guilin 541004, ChinaCollege of Chemistry and Biological Engineering, Guilin University of Technology, Guilin 541004, ChinaCollege of Chemistry and Biological Engineering, Guilin University of Technology, Guilin 541004, ChinaTo enhance the survival rate and release rate of probiotics in gastrointestinal fluids, hydrogels were prepared using citrus cellulose and sodium carboxymethylcellulose as embedding materials, with citric acid as the cross-linking agent. Lactiplantibacillus plantarum B5 was adsorbed and embedded in the hydrogels to evaluate the embedding rate, controlled release performance, bile salt tolerance, and storage stability. Results demonstrated that hydrogels with varying ratios exhibited favorable embedding effects, with an embedding rate exceeding 79%. The hydrogel with a citrus cellulose to sodium carboxymethylcellulose ratio of 1:4 showed optimal controlled release performance, achieving a release rate of 89.25% in simulated intestinal fluid. The bacteriophage-carrying hydrogel significantly enhanced the survival rate of L. plantarum B5 in bile salt solution, reaching 87.09%. The hydrogel maintained a storage stability of 30 days at 4 ℃, with the survival rate of embedded L. plantarum B5 exceeding 60% after 30 days, particularly reaching 70.66% with the 1:4 ratio of citrus cellulose to sodium carboxymethylcellulose, and maintaining viable bacteria levels above 7 lg CFU/mL. Studies have shown that citrus cellulose-sodium carboxymethylcellulose hydrogels are effective in reducing the exposure of probiotics to unfavorable factors in the external environment, thus significantly improving their survival in various environments.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024040044citrus cellulosehydrogellactiplantibacillus plantarumembeddingcontrolled release performancestorage stability
spellingShingle Shaoyang WANG
Ziyuan LI
Lina QIN
Xinyi CHEN
Liqi WANG
Tiemin JIANG
Haiyun LI
Preparation and Controlled Release Properties of Probiotic Hydrogel Carrier Based on Citrus Cellulose
Shipin gongye ke-ji
citrus cellulose
hydrogel
lactiplantibacillus plantarum
embedding
controlled release performance
storage stability
title Preparation and Controlled Release Properties of Probiotic Hydrogel Carrier Based on Citrus Cellulose
title_full Preparation and Controlled Release Properties of Probiotic Hydrogel Carrier Based on Citrus Cellulose
title_fullStr Preparation and Controlled Release Properties of Probiotic Hydrogel Carrier Based on Citrus Cellulose
title_full_unstemmed Preparation and Controlled Release Properties of Probiotic Hydrogel Carrier Based on Citrus Cellulose
title_short Preparation and Controlled Release Properties of Probiotic Hydrogel Carrier Based on Citrus Cellulose
title_sort preparation and controlled release properties of probiotic hydrogel carrier based on citrus cellulose
topic citrus cellulose
hydrogel
lactiplantibacillus plantarum
embedding
controlled release performance
storage stability
url http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2024040044
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AT linaqin preparationandcontrolledreleasepropertiesofprobiotichydrogelcarrierbasedoncitruscellulose
AT xinyichen preparationandcontrolledreleasepropertiesofprobiotichydrogelcarrierbasedoncitruscellulose
AT liqiwang preparationandcontrolledreleasepropertiesofprobiotichydrogelcarrierbasedoncitruscellulose
AT tieminjiang preparationandcontrolledreleasepropertiesofprobiotichydrogelcarrierbasedoncitruscellulose
AT haiyunli preparationandcontrolledreleasepropertiesofprobiotichydrogelcarrierbasedoncitruscellulose