Ammonifying and phosphorus-solubilizing function of Aliikangiella maris sp. nov. isolated from Phaeocystis globosa bloom and algal–bacterial interactions
Phaeocystis globosa blooms are of escalating global concern due to their substantial ecological impacts on marine ecosystems. Emerging evidence indicates that algae–bacterial interactions play pivotal roles in shaping the ecology and evolution of harmful algal blooms, although much of this interplay...
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Frontiers Media S.A.
2025-02-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1516993/full |
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| author | Fei Li Fei Li Ming-Ben Xu Liang-Hao Pan Jie Li Cai-Bi Lan Zhe Li Shan Lu Jun-Xiang Lai Jun-Xiang Lai Peng-Fu Li |
| author_facet | Fei Li Fei Li Ming-Ben Xu Liang-Hao Pan Jie Li Cai-Bi Lan Zhe Li Shan Lu Jun-Xiang Lai Jun-Xiang Lai Peng-Fu Li |
| author_sort | Fei Li |
| collection | DOAJ |
| description | Phaeocystis globosa blooms are of escalating global concern due to their substantial ecological impacts on marine ecosystems. Emerging evidence indicates that algae–bacterial interactions play pivotal roles in shaping the ecology and evolution of harmful algal blooms, although much of this interplay remains unexplored. We successfully isolated and propagated two novel bacterial strains from Phaeocystis globosa bloom. Two novel Gram-negative, non-spore-forming, motile, rod-shaped, and yellow-pigmented bacteria were designated strains GXAS 306T and GXAS 311. According to phenotypic, chemotaxonomic, phylogenomic, and comparative genomic analyses data, strains GXAS 306T and GXAS 311 were considered to represent a novel species of the genus Aliikangiella. Genomic analysis revealed that strain GXAS 306T had many potential functions favorable for interacting with algae, and further experimental evidence confirmed the ammonifying and phosphorus-solubilizing function. Co-culture experiments showed that strain GXAS 306T significantly improved algal growth parameters of two typical P. globosa strains (Pg293 and PgV01), particularly under nitrogen or phosphorus deficiency. Specifically, cell densities were observed to increase by 19.6–86.0%, accompanied by substantial enhancements in photosynthetic performance with increases of 8.0–30.6% in Fv/Fm and 10.9–27.9% in rETRmax. Overall, these results shed light on intricate relationships between P. globosa and its associated bacterial partners, which may influence the growth characteristics of algae. |
| format | Article |
| id | doaj-art-279255431f9c42d7bdd4ca61687836b6 |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-279255431f9c42d7bdd4ca61687836b62025-02-10T16:21:32ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-02-011610.3389/fmicb.2025.15169931516993Ammonifying and phosphorus-solubilizing function of Aliikangiella maris sp. nov. isolated from Phaeocystis globosa bloom and algal–bacterial interactionsFei Li0Fei Li1Ming-Ben Xu2Liang-Hao Pan3Jie Li4Cai-Bi Lan5Zhe Li6Shan Lu7Jun-Xiang Lai8Jun-Xiang Lai9Peng-Fu Li10State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, ChinaGuangxi Key Laboratory of Marine Environmental Science, Guangxi Academy of Marine Sciences, Guangxi Academy of Sciences, Nanning, ChinaGuangxi Key Laboratory of Marine Environmental Science, Guangxi Academy of Marine Sciences, Guangxi Academy of Sciences, Nanning, ChinaGuangxi Key Lab of Mangrove Conservation and Utilization, Guangxi Mangrove Research Center, Guangxi Academy of Marine Sciences, Beihai, ChinaGuangxi Key Laboratory of Marine Environmental Science, Guangxi Academy of Marine Sciences, Guangxi Academy of Sciences, Nanning, ChinaGuangxi Key Laboratory of Marine Environmental Science, Guangxi Academy of Marine Sciences, Guangxi Academy of Sciences, Nanning, ChinaGuangxi Key Laboratory of Marine Environmental Science, Guangxi Academy of Marine Sciences, Guangxi Academy of Sciences, Nanning, ChinaState Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, ChinaGuangxi Key Laboratory of Marine Environmental Science, Guangxi Academy of Marine Sciences, Guangxi Academy of Sciences, Nanning, ChinaBeibu Gulf Marine Industry Research Institute, Fangchenggang, ChinaState Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, ChinaPhaeocystis globosa blooms are of escalating global concern due to their substantial ecological impacts on marine ecosystems. Emerging evidence indicates that algae–bacterial interactions play pivotal roles in shaping the ecology and evolution of harmful algal blooms, although much of this interplay remains unexplored. We successfully isolated and propagated two novel bacterial strains from Phaeocystis globosa bloom. Two novel Gram-negative, non-spore-forming, motile, rod-shaped, and yellow-pigmented bacteria were designated strains GXAS 306T and GXAS 311. According to phenotypic, chemotaxonomic, phylogenomic, and comparative genomic analyses data, strains GXAS 306T and GXAS 311 were considered to represent a novel species of the genus Aliikangiella. Genomic analysis revealed that strain GXAS 306T had many potential functions favorable for interacting with algae, and further experimental evidence confirmed the ammonifying and phosphorus-solubilizing function. Co-culture experiments showed that strain GXAS 306T significantly improved algal growth parameters of two typical P. globosa strains (Pg293 and PgV01), particularly under nitrogen or phosphorus deficiency. Specifically, cell densities were observed to increase by 19.6–86.0%, accompanied by substantial enhancements in photosynthetic performance with increases of 8.0–30.6% in Fv/Fm and 10.9–27.9% in rETRmax. Overall, these results shed light on intricate relationships between P. globosa and its associated bacterial partners, which may influence the growth characteristics of algae.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1516993/fullAliikangiella marisPhaeocystis globosa bloomphycosphere bacteriumgenomealgal–bacterial interactions |
| spellingShingle | Fei Li Fei Li Ming-Ben Xu Liang-Hao Pan Jie Li Cai-Bi Lan Zhe Li Shan Lu Jun-Xiang Lai Jun-Xiang Lai Peng-Fu Li Ammonifying and phosphorus-solubilizing function of Aliikangiella maris sp. nov. isolated from Phaeocystis globosa bloom and algal–bacterial interactions Frontiers in Microbiology Aliikangiella maris Phaeocystis globosa bloom phycosphere bacterium genome algal–bacterial interactions |
| title | Ammonifying and phosphorus-solubilizing function of Aliikangiella maris sp. nov. isolated from Phaeocystis globosa bloom and algal–bacterial interactions |
| title_full | Ammonifying and phosphorus-solubilizing function of Aliikangiella maris sp. nov. isolated from Phaeocystis globosa bloom and algal–bacterial interactions |
| title_fullStr | Ammonifying and phosphorus-solubilizing function of Aliikangiella maris sp. nov. isolated from Phaeocystis globosa bloom and algal–bacterial interactions |
| title_full_unstemmed | Ammonifying and phosphorus-solubilizing function of Aliikangiella maris sp. nov. isolated from Phaeocystis globosa bloom and algal–bacterial interactions |
| title_short | Ammonifying and phosphorus-solubilizing function of Aliikangiella maris sp. nov. isolated from Phaeocystis globosa bloom and algal–bacterial interactions |
| title_sort | ammonifying and phosphorus solubilizing function of aliikangiella maris sp nov isolated from phaeocystis globosa bloom and algal bacterial interactions |
| topic | Aliikangiella maris Phaeocystis globosa bloom phycosphere bacterium genome algal–bacterial interactions |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1516993/full |
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