CB2 and TRPV1 receptors in inflammatory state of macrophages from sickle cell anemia pediatric/young adults
Abstract Sickle Cell Disease (SCD) is a monogenic disorder characterized by the production of abnormal hemoglobin. Polymerization of HbS causes sickling of red blood cells (RBCs) evidenced by acute adverse events and persistent inflammatory state, vasculopathy and organ damage. Sickled RBCs cause an...
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Nature Portfolio
2025-08-01
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| Online Access: | https://doi.org/10.1038/s41598-025-15028-2 |
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| author | Giuseppe Di Feo Giulia Giliberti Deeksha Rana-Seyfert Maria Maddalena Marrapodi Maddalena Casale Shakeel Ahmed Silverio Perrotta Francesca Rossi Domenico Roberti Alessandra Di Paola |
| author_facet | Giuseppe Di Feo Giulia Giliberti Deeksha Rana-Seyfert Maria Maddalena Marrapodi Maddalena Casale Shakeel Ahmed Silverio Perrotta Francesca Rossi Domenico Roberti Alessandra Di Paola |
| author_sort | Giuseppe Di Feo |
| collection | DOAJ |
| description | Abstract Sickle Cell Disease (SCD) is a monogenic disorder characterized by the production of abnormal hemoglobin. Polymerization of HbS causes sickling of red blood cells (RBCs) evidenced by acute adverse events and persistent inflammatory state, vasculopathy and organ damage. Sickled RBCs cause an anemic condition and vaso-occlusive crisis which trigger leukocytes, endothelial cells, and platelets. Due to these events, SCD patients unveiled an elevated level of pro-inflammatory cytokines, which contribute to the ongoing inflammatory state, oxidative stress, and other severe complications. SCD patients also experience neuropathic, inflammatory, and nociceptive pain. The discovery of novel therapeutic approaches and targets to counteract and manage inflammation in SCD are needed. Our study aimed to better understand the role of macrophages in SCD inflammation by first investigating their phenotype and then studying the iron metabolism involvement in the inflammatory processes. Therefore, given the importance to find novel therapeutic approach to contain and manage inflammation in these patients, and considering the role of CB2 and TRPV1 in this process, we decided to investigate the expression of these receptors and the effects of their stimulation on inflammatory state in SCD macrophages. |
| format | Article |
| id | doaj-art-26eb7378992e4a138e53bd30826af79f |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-26eb7378992e4a138e53bd30826af79f2025-08-20T03:43:02ZengNature PortfolioScientific Reports2045-23222025-08-0115111610.1038/s41598-025-15028-2CB2 and TRPV1 receptors in inflammatory state of macrophages from sickle cell anemia pediatric/young adultsGiuseppe Di Feo0Giulia Giliberti1Deeksha Rana-Seyfert2Maria Maddalena Marrapodi3Maddalena Casale4Shakeel Ahmed5Silverio Perrotta6Francesca Rossi7Domenico Roberti8Alessandra Di Paola9Department of Woman, Child and General and Specialist Surgery, University of Campania “Luigi Vanvitelli”Department of Advanced Medical and Surgical Sciences, University of Campania “Luigi Vanvitelli”Department of Advanced Medical and Surgical Sciences, University of Campania “Luigi Vanvitelli”Department of Woman, Child and General and Specialist Surgery, University of Campania “Luigi Vanvitelli”Department of Woman, Child and General and Specialist Surgery, University of Campania “Luigi Vanvitelli”Department of Advanced Medical and Surgical Sciences, University of Campania “Luigi Vanvitelli”Department of Woman, Child and General and Specialist Surgery, University of Campania “Luigi Vanvitelli”Department of Woman, Child and General and Specialist Surgery, University of Campania “Luigi Vanvitelli”Department of Woman, Child and General and Specialist Surgery, University of Campania “Luigi Vanvitelli”Department of Woman, Child and General and Specialist Surgery, University of Campania “Luigi Vanvitelli”Abstract Sickle Cell Disease (SCD) is a monogenic disorder characterized by the production of abnormal hemoglobin. Polymerization of HbS causes sickling of red blood cells (RBCs) evidenced by acute adverse events and persistent inflammatory state, vasculopathy and organ damage. Sickled RBCs cause an anemic condition and vaso-occlusive crisis which trigger leukocytes, endothelial cells, and platelets. Due to these events, SCD patients unveiled an elevated level of pro-inflammatory cytokines, which contribute to the ongoing inflammatory state, oxidative stress, and other severe complications. SCD patients also experience neuropathic, inflammatory, and nociceptive pain. The discovery of novel therapeutic approaches and targets to counteract and manage inflammation in SCD are needed. Our study aimed to better understand the role of macrophages in SCD inflammation by first investigating their phenotype and then studying the iron metabolism involvement in the inflammatory processes. Therefore, given the importance to find novel therapeutic approach to contain and manage inflammation in these patients, and considering the role of CB2 and TRPV1 in this process, we decided to investigate the expression of these receptors and the effects of their stimulation on inflammatory state in SCD macrophages.https://doi.org/10.1038/s41598-025-15028-2Sickle cell anemiaInflammationMacrophage polarizationIron metabolismEndocannabinoid/Endovanilloid system |
| spellingShingle | Giuseppe Di Feo Giulia Giliberti Deeksha Rana-Seyfert Maria Maddalena Marrapodi Maddalena Casale Shakeel Ahmed Silverio Perrotta Francesca Rossi Domenico Roberti Alessandra Di Paola CB2 and TRPV1 receptors in inflammatory state of macrophages from sickle cell anemia pediatric/young adults Scientific Reports Sickle cell anemia Inflammation Macrophage polarization Iron metabolism Endocannabinoid/Endovanilloid system |
| title | CB2 and TRPV1 receptors in inflammatory state of macrophages from sickle cell anemia pediatric/young adults |
| title_full | CB2 and TRPV1 receptors in inflammatory state of macrophages from sickle cell anemia pediatric/young adults |
| title_fullStr | CB2 and TRPV1 receptors in inflammatory state of macrophages from sickle cell anemia pediatric/young adults |
| title_full_unstemmed | CB2 and TRPV1 receptors in inflammatory state of macrophages from sickle cell anemia pediatric/young adults |
| title_short | CB2 and TRPV1 receptors in inflammatory state of macrophages from sickle cell anemia pediatric/young adults |
| title_sort | cb2 and trpv1 receptors in inflammatory state of macrophages from sickle cell anemia pediatric young adults |
| topic | Sickle cell anemia Inflammation Macrophage polarization Iron metabolism Endocannabinoid/Endovanilloid system |
| url | https://doi.org/10.1038/s41598-025-15028-2 |
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