Consistent trends from different methods for monitoring SARS-CoV-2 in urban wastewater during a 29-month longitudinal study

Consistent trends from different methods for monitoring SARS-CoV-2 in urban wastewater during a 29-month longitudinal study

Rigorous method development and validation to detect and quantify SARS-CoV-2 RNA in wastewater has led to important advances in community disease surveillance using quantitative molecular biology tools. Despite this progress, agreement on standardized workflows for this important public health objec...

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Main Authors: Janine McCalder, Jangwoo Lee, Judy Qiu, Qiaozhi Li, Linnet Immaraj, Nicole Acosta, María A. Bautista, Melissa Wilson, Barbara Waddell, Kristine Du, Aito Ueno, Rhonda Clark, Alexander Krusina, Danielle A. Southern, Tyler Williamson, Chloe Papparis, Paul Montesclaros, Lance Non, September Stefani, Gail Visser, Puja Pradhan, Norma Ruecker, John Conly, Steve Hrudey, Kevin Frankowski, Bonita Lee, Michael D. Parkins, Xiaoli Pang, Casey R. J. Hubert
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-06-01
Series:Frontiers in Microbiology
Subjects:
SARS-CoV-2
wastewater
wastewater-based epidemiology
concentration method
direct extraction
method comparison
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2025.1547831/full
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https://www.frontiersin.org/articles/10.3389/fmicb.2025.1547831/full

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