<i>HOM2</i> Deletion by CRISPR-Cas9 in <i>Saccharomyces cerevisiae</i> for Decreasing Higher Alcohols in Whiskey

<i>HOM2</i> Deletion by CRISPR-Cas9 in <i>Saccharomyces cerevisiae</i> for Decreasing Higher Alcohols in Whiskey

In typical whiskey, the content of higher alcohols is about 1500–2000 mg/L, leading to a high intoxicating degree (ID). To produce low-ID whiskey, <i>Saccharomyces cerevisiae</i> XF0-h, XF0-H and XF0-LH were successfully constructed by CRISPR-Cas9 gene editing technology to knockout <...

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Bibliographic Details
Main Authors: Jiaojiao He, Haoyang Zhou, Jine Liang, Kadireya Tuerxun, Zhuoling Ding, Shishui Zhou
Format: Article
Language:English
Published: MDPI AG 2024-11-01
Series:Fermentation
Subjects:
CRISPR-Cas9
<i>HOM2</i> gene
higher alcohols
whiskey
Box–Behnken design
Online Access:https://www.mdpi.com/2311-5637/10/11/589
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