Dexamethasone-Activated MSCs Release MVs for Stimulating Osteogenic Response
The extracellular microvesicles (MVs) are attracting much attention because they are found to be the key paracrine mediator participating in tissue regeneration. Dexamethasone (DXM) is widely accepted as an important regulator in tailoring the differentiation potential of mesenchymal stem cells (MSC...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2018/7231739 |
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| _version_ | 1832548590580400128 |
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| author | Mingyan Zhao Peng Li Haijia Xu Qunwen Pan Rong Zeng Xiaotang Ma Zhanghua Li Hao Lin |
| author_facet | Mingyan Zhao Peng Li Haijia Xu Qunwen Pan Rong Zeng Xiaotang Ma Zhanghua Li Hao Lin |
| author_sort | Mingyan Zhao |
| collection | DOAJ |
| description | The extracellular microvesicles (MVs) are attracting much attention because they are found to be the key paracrine mediator participating in tissue regeneration. Dexamethasone (DXM) is widely accepted as an important regulator in tailoring the differentiation potential of mesenchymal stem cells (MSCs). However, the effect of DXM on the paracrine signaling of MSCs remains unknown. To this point, we aimed to explore the role of DXM in regulating the paracrine activity of MSCs through evaluating the release and function of MSC-MVs, based on their physicochemical characteristics and support on osteogenic response. Results showed that DXM had no evident impact on the release of MSC-MVs but played a pivotal role in regulating the function of MSC-MVs. MVs obtained from the DXM-stimulated MSCs (DXM-MVs) increased MC3T3 cell proliferation and migration and upregulated Runt-related transcription factor 2 (Runx2), alkaline phosphatase (ALP), and osteopontin (OPN) expression. The repair efficiency of DXM-MVs for femur defects was further investigated in an established rat model. It was found that DXM-MVs accelerated the healing process of bone formation in the defect area. Thus, we conclude that using DXM as stimuli to obtain functional MSCs-MVs could become a valuable tool for promoting bone regeneration. |
| format | Article |
| id | doaj-art-25b1b7273bf84177b9b9e659e6ead8ad |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-25b1b7273bf84177b9b9e659e6ead8ad2025-02-03T06:13:39ZengWileyStem Cells International1687-966X1687-96782018-01-01201810.1155/2018/72317397231739Dexamethasone-Activated MSCs Release MVs for Stimulating Osteogenic ResponseMingyan Zhao0Peng Li1Haijia Xu2Qunwen Pan3Rong Zeng4Xiaotang Ma5Zhanghua Li6Hao Lin7Stem Cell Research and Cellular Therapy Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, ChinaStem Cell Research and Cellular Therapy Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, ChinaDepartment of Orthopaedics, Tongren Hospital of Wuhan University, Wuhan 430060, ChinaDepartment of Surgery, Guangdong Key Laboratory of Age-Related Cardiac and Cerebral Diseases, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, ChinaDepartment of Spinal Surgery, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, ChinaDepartment of Surgery, Guangdong Key Laboratory of Age-Related Cardiac and Cerebral Diseases, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, ChinaDepartment of Orthopaedics, Tongren Hospital of Wuhan University, Wuhan 430060, ChinaDepartment of Spinal Surgery, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, ChinaThe extracellular microvesicles (MVs) are attracting much attention because they are found to be the key paracrine mediator participating in tissue regeneration. Dexamethasone (DXM) is widely accepted as an important regulator in tailoring the differentiation potential of mesenchymal stem cells (MSCs). However, the effect of DXM on the paracrine signaling of MSCs remains unknown. To this point, we aimed to explore the role of DXM in regulating the paracrine activity of MSCs through evaluating the release and function of MSC-MVs, based on their physicochemical characteristics and support on osteogenic response. Results showed that DXM had no evident impact on the release of MSC-MVs but played a pivotal role in regulating the function of MSC-MVs. MVs obtained from the DXM-stimulated MSCs (DXM-MVs) increased MC3T3 cell proliferation and migration and upregulated Runt-related transcription factor 2 (Runx2), alkaline phosphatase (ALP), and osteopontin (OPN) expression. The repair efficiency of DXM-MVs for femur defects was further investigated in an established rat model. It was found that DXM-MVs accelerated the healing process of bone formation in the defect area. Thus, we conclude that using DXM as stimuli to obtain functional MSCs-MVs could become a valuable tool for promoting bone regeneration.http://dx.doi.org/10.1155/2018/7231739 |
| spellingShingle | Mingyan Zhao Peng Li Haijia Xu Qunwen Pan Rong Zeng Xiaotang Ma Zhanghua Li Hao Lin Dexamethasone-Activated MSCs Release MVs for Stimulating Osteogenic Response Stem Cells International |
| title | Dexamethasone-Activated MSCs Release MVs for Stimulating Osteogenic Response |
| title_full | Dexamethasone-Activated MSCs Release MVs for Stimulating Osteogenic Response |
| title_fullStr | Dexamethasone-Activated MSCs Release MVs for Stimulating Osteogenic Response |
| title_full_unstemmed | Dexamethasone-Activated MSCs Release MVs for Stimulating Osteogenic Response |
| title_short | Dexamethasone-Activated MSCs Release MVs for Stimulating Osteogenic Response |
| title_sort | dexamethasone activated mscs release mvs for stimulating osteogenic response |
| url | http://dx.doi.org/10.1155/2018/7231739 |
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