Guiding the Differentiation Direction of Pancreatic Islet-Derived Stem Cells by Glycated Collagen
The microenvironment is an important factor of stem cells regulating their maintenance, survival, and differentiation. The glycation of proteins with reducing sugars through nonenzymatic reactions induces the collagen cross-linking, which causes tissue stiffening, which is enhanced during aging and...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2018/6143081 |
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| author | Gokhan Duruksu Aysegul Aciksari |
| author_facet | Gokhan Duruksu Aysegul Aciksari |
| author_sort | Gokhan Duruksu |
| collection | DOAJ |
| description | The microenvironment is an important factor of stem cells regulating their maintenance, survival, and differentiation. The glycation of proteins with reducing sugars through nonenzymatic reactions induces the collagen cross-linking, which causes tissue stiffening, which is enhanced during aging and diabetes. In this study, we aimed to analyze the effect of glycated collagen on the stem cell culture and differentiation. The collagen type 1 was modified by glycation with mannose, rhamnose, arabinose, and glucose. After the culture of mesenchymal stem cells on the coated surfaces with glycated collagen, the differences in cell adhesion, proliferation, and differentiation were compared. The results showed that the modifications did not induce apoptosis or cause cell death. However, the culture of cells on modified collagens improved the proliferation. It was found that the mannose-modified collagen stimulated the adipogenic differentiation of stem cells, and rhamnose-modified collagen supports the differentiation into both osteogenic and insulin-producing cells. The low concentration of monosaccharides during glycation process improved the characteristics of the matrix protein in favor of stem cell differentiation. Modification of the collagen by glycation might be used as a tool to improve natural polymers for material-induced stem cell differentiation in the future. |
| format | Article |
| id | doaj-art-256857cf4a3b4b7191f4f3d89a78f9c1 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-256857cf4a3b4b7191f4f3d89a78f9c12025-02-03T01:07:45ZengWileyStem Cells International1687-966X1687-96782018-01-01201810.1155/2018/61430816143081Guiding the Differentiation Direction of Pancreatic Islet-Derived Stem Cells by Glycated CollagenGokhan Duruksu0Aysegul Aciksari1Center for Stem Cell and Gene Therapies Research and Practice, Kocaeli University, 41380 Izmit, Kocaeli, TurkeyInstitute of Health Sciences, Stem Cell Department, Kocaeli University, 41380 Izmit, Kocaeli, TurkeyThe microenvironment is an important factor of stem cells regulating their maintenance, survival, and differentiation. The glycation of proteins with reducing sugars through nonenzymatic reactions induces the collagen cross-linking, which causes tissue stiffening, which is enhanced during aging and diabetes. In this study, we aimed to analyze the effect of glycated collagen on the stem cell culture and differentiation. The collagen type 1 was modified by glycation with mannose, rhamnose, arabinose, and glucose. After the culture of mesenchymal stem cells on the coated surfaces with glycated collagen, the differences in cell adhesion, proliferation, and differentiation were compared. The results showed that the modifications did not induce apoptosis or cause cell death. However, the culture of cells on modified collagens improved the proliferation. It was found that the mannose-modified collagen stimulated the adipogenic differentiation of stem cells, and rhamnose-modified collagen supports the differentiation into both osteogenic and insulin-producing cells. The low concentration of monosaccharides during glycation process improved the characteristics of the matrix protein in favor of stem cell differentiation. Modification of the collagen by glycation might be used as a tool to improve natural polymers for material-induced stem cell differentiation in the future.http://dx.doi.org/10.1155/2018/6143081 |
| spellingShingle | Gokhan Duruksu Aysegul Aciksari Guiding the Differentiation Direction of Pancreatic Islet-Derived Stem Cells by Glycated Collagen Stem Cells International |
| title | Guiding the Differentiation Direction of Pancreatic Islet-Derived Stem Cells by Glycated Collagen |
| title_full | Guiding the Differentiation Direction of Pancreatic Islet-Derived Stem Cells by Glycated Collagen |
| title_fullStr | Guiding the Differentiation Direction of Pancreatic Islet-Derived Stem Cells by Glycated Collagen |
| title_full_unstemmed | Guiding the Differentiation Direction of Pancreatic Islet-Derived Stem Cells by Glycated Collagen |
| title_short | Guiding the Differentiation Direction of Pancreatic Islet-Derived Stem Cells by Glycated Collagen |
| title_sort | guiding the differentiation direction of pancreatic islet derived stem cells by glycated collagen |
| url | http://dx.doi.org/10.1155/2018/6143081 |
| work_keys_str_mv | AT gokhanduruksu guidingthedifferentiationdirectionofpancreaticisletderivedstemcellsbyglycatedcollagen AT aysegulaciksari guidingthedifferentiationdirectionofpancreaticisletderivedstemcellsbyglycatedcollagen |