Second-hand Smoke Increases Nitric Oxide and Alters the IgE Response in a Murine Model of Allergic Aspergillosis
This study was performed to determine the effects of environmental tobacco smoke (ETS) on nitric oxide (NO) and immunoglobulin (Ig) production in a murine model of allergic bronchopulmonary aspergillosis (ABPA). Adult BALB/c mice were exposed to aged and dilute...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2005-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1080/17402520500116806 |
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| Summary: | This study was performed to determine the effects of environmental
tobacco smoke (ETS) on nitric oxide (NO) and immunoglobulin (Ig) production in
a murine model of allergic bronchopulmonary aspergillosis (ABPA). Adult
BALB/c mice were exposed to aged and diluted sidestream cigarette smoke
from day 0 through day 43 to simulate “second-hand
smoke”. During exposure,
mice were sensitized to soluble Aspergillus fumigatus (Af)
antigen intranasally
between day 14 and 24. All Af sensitized mice in ambient air (Af + AIR) made
elevated levels of IgE, IgG1, IgM, IgG2a and IgA. Af sensitized mice housed in
ETS (Af + ETS) made similar levels of immunoglobulins except for IgE that was
significantly reduced in the serum and bronchoalveolar lavage (BAL). However,
immunohistochemical evaluation of the lung revealed a marked accumulation of
IgE positive cells in the lung parenchyma of these Af + ETS mice. LPS stimulation
of BAL cells revealed elevated levels of NO in the Af + AIR group, which was further
enhanced in the Af+ETS group. In vitro restimulation of the BAL cells on day 45
showed a TH0 response with elevated levels of IL3, 4, 5, 10 and IFN-γ. However,
by day 28 the response shifted such that TH2 cytokines increased while
IFN-γ decreased. The Af + ETS group showed markedly reduced levels in all
cytokines tested, including the inflammatory cytokine IL6, when compared to
the Af+AIR group. These results demonstrate that ETS affects ABPA by further
enhancing the NO production and reduces
the TH2 and the inflammatory cytokines while altering the pattern of IgE responses. |
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| ISSN: | 1740-2522 1740-2530 |