Comparative evaluation of sequencing technologies and primer sets for mouse gut microbiota profiling

Comparative evaluation of sequencing technologies and primer sets for mouse gut microbiota profiling

BackgroundAdvancements in sequencing technologies, such as Illumina and Oxford Nanopore Technologies (ONT), have significantly improved microbiome research. However, variations in sequencing platforms, primer selection, and DNA quality may influence microbial diversity assessments, particularly in s...

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Main Authors: Aleksandra Strokach, Polina Zoruk, Daria Boldyreva, Maxim Morozov, Evgenii Olekhnovich, Vladimir Veselovsky, Vladislav Babenko, Oksana Selezneva, Natalia Zakharevich, Andrey Larin, Severina Koldman, Vail Koldman, Maya Odorskaya, Roman Yunes, Vladislav Pavlov, Anna Kudryavtseva, Valeriy Danilenko, Ksenia Klimina
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-05-01
Series:Frontiers in Microbiology
Subjects:
gut microbiota
sequencing technologies
metagenome sequencing
Oxford Nanopore Technologies
Illumina sequencing
microbial diversity
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2025.1584359/full
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https://www.frontiersin.org/articles/10.3389/fmicb.2025.1584359/full

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