Determination of Tenacissoside G, Tenacissoside H, and Tenacissoside I in Rat Plasma by UPLC-MS/MS and Their Pharmacokinetics
An ultra-performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) method was developed for the determination of tenacissoside G, tenacissoside H, and tenacissoside I in rat plasma. The rat plasma was treated with liquid-liquid extraction using ethyl acetate. The determination was perf...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-01-01
|
| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2023/4747771 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832547343499526144 |
|---|---|
| author | Fan Chen Yizhe Ma Ying Cui Wanhang Wang Chenchen Mei Jingjing Nie Congcong Wen Xiuwei Shen Xuzhao Zhou |
| author_facet | Fan Chen Yizhe Ma Ying Cui Wanhang Wang Chenchen Mei Jingjing Nie Congcong Wen Xiuwei Shen Xuzhao Zhou |
| author_sort | Fan Chen |
| collection | DOAJ |
| description | An ultra-performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) method was developed for the determination of tenacissoside G, tenacissoside H, and tenacissoside I in rat plasma. The rat plasma was treated with liquid-liquid extraction using ethyl acetate. The determination was performed on the UPLC HSS T3 column (50 mm × 2.1 mm, 1.8 μm) with a mobile phase consisting of acetonitrile-water (containing 0.1% formic acid) and gradient elution at a flow rate of 0.4 mL/min. Electrospray (ESI) positive ion mode detection and multireaction monitoring (MRM) quantitative analysis were performed. A total of 36 rats were given tenacissoside G, tenacissoside H, and tenacissoside I, respectively, orally (5 mg/kg) and intravenously (1 mg/kg), with 6 rats in each group, to evaluate the pharmacokinetic difference of tenacissoside G, tenacissoside H, and tenacissoside I in rats. The calibration curves showed good linearity in the range of 5–2000 ng/mL, where r was greater than 0.99. The results of precision, accuracy, recovery, matrix effect, and stability met the requirements of biological sample detection methods. The established UPLC-MS/MS method was successfully applied to pharmacokinetic studies of tenacissoside G, tenacissoside H, and tenacissoside I, and the bioavailability was 22.9%, 89.8%, and 9.4%, respectively. |
| format | Article |
| id | doaj-art-240dc35daf1b42e4a59bf4b4b232a645 |
| institution | Kabale University |
| issn | 1687-8779 |
| language | English |
| publishDate | 2023-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-240dc35daf1b42e4a59bf4b4b232a6452025-02-03T06:45:14ZengWileyInternational Journal of Analytical Chemistry1687-87792023-01-01202310.1155/2023/4747771Determination of Tenacissoside G, Tenacissoside H, and Tenacissoside I in Rat Plasma by UPLC-MS/MS and Their PharmacokineticsFan Chen0Yizhe Ma1Ying Cui2Wanhang Wang3Chenchen Mei4Jingjing Nie5Congcong Wen6Xiuwei Shen7Xuzhao Zhou8Ruian People’s HospitalLaboratory Animal CentreLaboratory Animal CentreLaboratory Animal CentreLaboratory Animal CentreRuian People’s HospitalLaboratory Animal CentreRuian People’s HospitalThe Molecular Neuropharmacology Laboratory and the Eye-Brain Research CenterAn ultra-performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) method was developed for the determination of tenacissoside G, tenacissoside H, and tenacissoside I in rat plasma. The rat plasma was treated with liquid-liquid extraction using ethyl acetate. The determination was performed on the UPLC HSS T3 column (50 mm × 2.1 mm, 1.8 μm) with a mobile phase consisting of acetonitrile-water (containing 0.1% formic acid) and gradient elution at a flow rate of 0.4 mL/min. Electrospray (ESI) positive ion mode detection and multireaction monitoring (MRM) quantitative analysis were performed. A total of 36 rats were given tenacissoside G, tenacissoside H, and tenacissoside I, respectively, orally (5 mg/kg) and intravenously (1 mg/kg), with 6 rats in each group, to evaluate the pharmacokinetic difference of tenacissoside G, tenacissoside H, and tenacissoside I in rats. The calibration curves showed good linearity in the range of 5–2000 ng/mL, where r was greater than 0.99. The results of precision, accuracy, recovery, matrix effect, and stability met the requirements of biological sample detection methods. The established UPLC-MS/MS method was successfully applied to pharmacokinetic studies of tenacissoside G, tenacissoside H, and tenacissoside I, and the bioavailability was 22.9%, 89.8%, and 9.4%, respectively.http://dx.doi.org/10.1155/2023/4747771 |
| spellingShingle | Fan Chen Yizhe Ma Ying Cui Wanhang Wang Chenchen Mei Jingjing Nie Congcong Wen Xiuwei Shen Xuzhao Zhou Determination of Tenacissoside G, Tenacissoside H, and Tenacissoside I in Rat Plasma by UPLC-MS/MS and Their Pharmacokinetics International Journal of Analytical Chemistry |
| title | Determination of Tenacissoside G, Tenacissoside H, and Tenacissoside I in Rat Plasma by UPLC-MS/MS and Their Pharmacokinetics |
| title_full | Determination of Tenacissoside G, Tenacissoside H, and Tenacissoside I in Rat Plasma by UPLC-MS/MS and Their Pharmacokinetics |
| title_fullStr | Determination of Tenacissoside G, Tenacissoside H, and Tenacissoside I in Rat Plasma by UPLC-MS/MS and Their Pharmacokinetics |
| title_full_unstemmed | Determination of Tenacissoside G, Tenacissoside H, and Tenacissoside I in Rat Plasma by UPLC-MS/MS and Their Pharmacokinetics |
| title_short | Determination of Tenacissoside G, Tenacissoside H, and Tenacissoside I in Rat Plasma by UPLC-MS/MS and Their Pharmacokinetics |
| title_sort | determination of tenacissoside g tenacissoside h and tenacissoside i in rat plasma by uplc ms ms and their pharmacokinetics |
| url | http://dx.doi.org/10.1155/2023/4747771 |
| work_keys_str_mv | AT fanchen determinationoftenacissosidegtenacissosidehandtenacissosideiinratplasmabyuplcmsmsandtheirpharmacokinetics AT yizhema determinationoftenacissosidegtenacissosidehandtenacissosideiinratplasmabyuplcmsmsandtheirpharmacokinetics AT yingcui determinationoftenacissosidegtenacissosidehandtenacissosideiinratplasmabyuplcmsmsandtheirpharmacokinetics AT wanhangwang determinationoftenacissosidegtenacissosidehandtenacissosideiinratplasmabyuplcmsmsandtheirpharmacokinetics AT chenchenmei determinationoftenacissosidegtenacissosidehandtenacissosideiinratplasmabyuplcmsmsandtheirpharmacokinetics AT jingjingnie determinationoftenacissosidegtenacissosidehandtenacissosideiinratplasmabyuplcmsmsandtheirpharmacokinetics AT congcongwen determinationoftenacissosidegtenacissosidehandtenacissosideiinratplasmabyuplcmsmsandtheirpharmacokinetics AT xiuweishen determinationoftenacissosidegtenacissosidehandtenacissosideiinratplasmabyuplcmsmsandtheirpharmacokinetics AT xuzhaozhou determinationoftenacissosidegtenacissosidehandtenacissosideiinratplasmabyuplcmsmsandtheirpharmacokinetics |