Protocol for the analysis of cell-free DNA end characteristics for accurate cancer diagnosis

Summary: Fragmentation patterns of plasma cell-free DNA (cfDNA) are promising biomarkers in cancer diagnosis. Here, we present a protocol to enrich tumor-derived cfDNA molecules through end selection. We describe steps for installing software, aligning plasma cfDNA data to the reference genome, and...

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Bibliographic Details
Main Authors: Xiaoyi Liu, Fanglei Gong, Huizhen Lin, Yunyun An, Kun Sun
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:STAR Protocols
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Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725001637
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