Dual-functional probiotic hydrogel with puerarin integration for microbiota-neuroimmune regulation in antibiotic-free periodontitis therapy
Periodontitis is an inflammatory disease caused by the imbalance of the periodontal microbial ecosystem. Traditional treatment methods not only kill pathogenic bacteria but also inhibit the growth of beneficial bacteria, thereby disrupting the balance of the oral microbial ecosystem. In this study,...
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KeAi Communications Co., Ltd.
2025-11-01
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| Series: | Bioactive Materials |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2452199X2500297X |
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| author | Yi Wang Yansheng Qu Xiangmei Liu Jin Huang Congyang Mao Chaofeng Wang Shengli Zhu Yufeng Zheng Zhaoyang Li Zhenduo Cui Hui Jiang Yang Lv Jie Shen Shuilin Wu |
| author_facet | Yi Wang Yansheng Qu Xiangmei Liu Jin Huang Congyang Mao Chaofeng Wang Shengli Zhu Yufeng Zheng Zhaoyang Li Zhenduo Cui Hui Jiang Yang Lv Jie Shen Shuilin Wu |
| author_sort | Yi Wang |
| collection | DOAJ |
| description | Periodontitis is an inflammatory disease caused by the imbalance of the periodontal microbial ecosystem. Traditional treatment methods not only kill pathogenic bacteria but also inhibit the growth of beneficial bacteria, thereby disrupting the balance of the oral microbial ecosystem. In this study, chitosan, hyaluronic acid, and puerarin were coated on live Lactobacillus rhamnosus to form probiotic-based PSCLR nanoparticles. Subsequently, these nanoparticles were encapsulated in hydrogel microspheres through emulsion polymerization and immobilized in the hydrogel network via the covalent cross-linking of methacrylate-modified hyaluronic acid. This hydrogel restores the balance of oral microbiota by reducing pathogenic bacteria and promoting probiotic diversity, which helps maintain ecological stability and minimize risks associated with microbial community dysbiosis. In addition, puerarin in the gel achieves analgesic and neuromodulatory effects by specifically antagonizing the P2X3 receptor, which affects pain transmission. This study provides a potential strategy for the antibiotic-free treatment of periodontitis and the neuropathic pain associated with it. |
| format | Article |
| id | doaj-art-22f22002badd467eb4affd31e52a47e9 |
| institution | OA Journals |
| issn | 2452-199X |
| language | English |
| publishDate | 2025-11-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Bioactive Materials |
| spelling | doaj-art-22f22002badd467eb4affd31e52a47e92025-08-20T02:36:13ZengKeAi Communications Co., Ltd.Bioactive Materials2452-199X2025-11-0153728310.1016/j.bioactmat.2025.07.004Dual-functional probiotic hydrogel with puerarin integration for microbiota-neuroimmune regulation in antibiotic-free periodontitis therapyYi Wang0Yansheng Qu1Xiangmei Liu2Jin Huang3Congyang Mao4Chaofeng Wang5Shengli Zhu6Yufeng Zheng7Zhaoyang Li8Zhenduo Cui9Hui Jiang10Yang Lv11Jie Shen12Shuilin Wu13Biomedical Materials Engineering Research Center, Hubei Key Laboratory of Polymer Materials, Ministry-of-Education Key Laboratory for the Green Preparation and Application of Functional Materials, School of Materials Science & Engineering, Hubei University, Wuhan, 430062, China; School of Health Science & Biomedical Engineering, Hebei University of Technology, Tianjin, 300401, China; School of Materials Science & Engineering, Peking University, Beijing, 100871, ChinaBiomedical Materials Engineering Research Center, Hubei Key Laboratory of Polymer Materials, Ministry-of-Education Key Laboratory for the Green Preparation and Application of Functional Materials, School of Materials Science & Engineering, Hubei University, Wuhan, 430062, ChinaBiomedical Materials Engineering Research Center, Hubei Key Laboratory of Polymer Materials, Ministry-of-Education Key Laboratory for the Green Preparation and Application of Functional Materials, School of Materials Science & Engineering, Hubei University, Wuhan, 430062, China; School of Health Science & Biomedical Engineering, Hebei University of Technology, Tianjin, 300401, China; Corresponding author. Biomedical Materials Engineering Research Center, Hubei Key Laboratory of Polymer Materials, Ministry-of-Education Key Laboratory for the Green Preparation and Application of Functional Materials, School of Materials Science & Engineering, Hubei University, Wuhan, 430062, China.Biomedical Materials Engineering Research Center, Hubei Key Laboratory of Polymer Materials, Ministry-of-Education Key Laboratory for the Green Preparation and Application of Functional Materials, School of Materials Science & Engineering, Hubei University, Wuhan, 430062, China; School of Health Science & Biomedical Engineering, Hebei University of Technology, Tianjin, 300401, China; School of Materials Science & Engineering, Peking University, Beijing, 100871, ChinaBiomedical Materials Engineering Research Center, Hubei Key Laboratory of Polymer Materials, Ministry-of-Education Key Laboratory for the Green Preparation and Application of Functional Materials, School of Materials Science & Engineering, Hubei University, Wuhan, 430062, ChinaSchool of Health Science & Biomedical Engineering, Hebei University of Technology, Tianjin, 300401, ChinaSchool of Materials Science & Engineering, The Key Laboratory of Advanced Ceramics and Machining Technology By the Ministry of Education of China, Tianjin University, Tianjin, 300072, ChinaSchool of Materials Science & Engineering, Peking University, Beijing, 100871, ChinaSchool of Materials Science & Engineering, The Key Laboratory of Advanced Ceramics and Machining Technology By the Ministry of Education of China, Tianjin University, Tianjin, 300072, ChinaSchool of Materials Science & Engineering, The Key Laboratory of Advanced Ceramics and Machining Technology By the Ministry of Education of China, Tianjin University, Tianjin, 300072, ChinaSchool of Materials Science & Engineering, The Key Laboratory of Advanced Ceramics and Machining Technology By the Ministry of Education of China, Tianjin University, Tianjin, 300072, ChinaDepartment of Orthopedics, Engineering Research Center of Bone and Joint Precision Medicine, Ministry of Education, Peking University Third Hospital, Beijing, 100191, China; Corresponding author. Department of Orthopedics, Engineering Research Center of Bone and Joint Precision Medicine, Ministry of Education, Peking University Third Hospital, Beijing, 100191, China.Shenzhen Key Laboratory of Spine Surgery, Department of Spine Surgery, Peking University Shenzhen Hospital, Shenzhen, 518036, ChinaBiomedical Materials Engineering Research Center, Hubei Key Laboratory of Polymer Materials, Ministry-of-Education Key Laboratory for the Green Preparation and Application of Functional Materials, School of Materials Science & Engineering, Hubei University, Wuhan, 430062, China; School of Materials Science & Engineering, Peking University, Beijing, 100871, China; Corresponding author. Biomedical Materials Engineering Research Center, Hubei Key Laboratory of Polymer Materials, Ministry-of-Education Key Laboratory for the Green Preparation and Application of Functional Materials, School of Materials Science & Engineering, Hubei University, Wuhan, 430062, China.Periodontitis is an inflammatory disease caused by the imbalance of the periodontal microbial ecosystem. Traditional treatment methods not only kill pathogenic bacteria but also inhibit the growth of beneficial bacteria, thereby disrupting the balance of the oral microbial ecosystem. In this study, chitosan, hyaluronic acid, and puerarin were coated on live Lactobacillus rhamnosus to form probiotic-based PSCLR nanoparticles. Subsequently, these nanoparticles were encapsulated in hydrogel microspheres through emulsion polymerization and immobilized in the hydrogel network via the covalent cross-linking of methacrylate-modified hyaluronic acid. This hydrogel restores the balance of oral microbiota by reducing pathogenic bacteria and promoting probiotic diversity, which helps maintain ecological stability and minimize risks associated with microbial community dysbiosis. In addition, puerarin in the gel achieves analgesic and neuromodulatory effects by specifically antagonizing the P2X3 receptor, which affects pain transmission. This study provides a potential strategy for the antibiotic-free treatment of periodontitis and the neuropathic pain associated with it.http://www.sciencedirect.com/science/article/pii/S2452199X2500297XProbioticsPuerarinHydrogelAntibacterialPeriodontitis |
| spellingShingle | Yi Wang Yansheng Qu Xiangmei Liu Jin Huang Congyang Mao Chaofeng Wang Shengli Zhu Yufeng Zheng Zhaoyang Li Zhenduo Cui Hui Jiang Yang Lv Jie Shen Shuilin Wu Dual-functional probiotic hydrogel with puerarin integration for microbiota-neuroimmune regulation in antibiotic-free periodontitis therapy Bioactive Materials Probiotics Puerarin Hydrogel Antibacterial Periodontitis |
| title | Dual-functional probiotic hydrogel with puerarin integration for microbiota-neuroimmune regulation in antibiotic-free periodontitis therapy |
| title_full | Dual-functional probiotic hydrogel with puerarin integration for microbiota-neuroimmune regulation in antibiotic-free periodontitis therapy |
| title_fullStr | Dual-functional probiotic hydrogel with puerarin integration for microbiota-neuroimmune regulation in antibiotic-free periodontitis therapy |
| title_full_unstemmed | Dual-functional probiotic hydrogel with puerarin integration for microbiota-neuroimmune regulation in antibiotic-free periodontitis therapy |
| title_short | Dual-functional probiotic hydrogel with puerarin integration for microbiota-neuroimmune regulation in antibiotic-free periodontitis therapy |
| title_sort | dual functional probiotic hydrogel with puerarin integration for microbiota neuroimmune regulation in antibiotic free periodontitis therapy |
| topic | Probiotics Puerarin Hydrogel Antibacterial Periodontitis |
| url | http://www.sciencedirect.com/science/article/pii/S2452199X2500297X |
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