Identification of markers of saccharides in Polygonati rhizoma during the steaming process by MALDI-Q-TOF mass spectrometry imaging
Polygonati rhizoma is often used in Chinese medicine and as food. In this study, atmospheric pressure matrix-assisted laser desorption ionization and quadruple-time-of-flight (MALDI-Q-TOF) mass spectrometry techniques were applied to P. rhizoma samples from Polygonatum cyrtonema Hua species. Positiv...
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Tsinghua University Press
2025-04-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | https://www.sciopen.com/article/10.26599/FSHW.2024.9250090 |
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| author | Chuang Liu Juan Chen Jia Lao You Qin Ziling Wang Zhenni Xie Wei He Can Zhong Shuihan Zhang Jian Jin |
| author_facet | Chuang Liu Juan Chen Jia Lao You Qin Ziling Wang Zhenni Xie Wei He Can Zhong Shuihan Zhang Jian Jin |
| author_sort | Chuang Liu |
| collection | DOAJ |
| description | Polygonati rhizoma is often used in Chinese medicine and as food. In this study, atmospheric pressure matrix-assisted laser desorption ionization and quadruple-time-of-flight (MALDI-Q-TOF) mass spectrometry techniques were applied to P. rhizoma samples from Polygonatum cyrtonema Hua species. Positive ions were mainly detected in the mass range of m/z 200–600, while negative ions were mainly observed in the mass range of m/z 100–450. A total of 263 components were identified and the spatial distribution and changes in saccharides contents during the steaming process of P. rhizoma were investigated. Monosaccharide and disaccharide exhibit a relatively uniform distribution, while the oligosaccharides were mainly found in the bast of fresh P. rhizoma. Although the contents of monosaccharide and disaccharide were increased during steaming, that of trisaccharide, tetrasaccharide, and pentasaccharide were decreased. We used the 5 saccharide types with the greatest variation in content as variables for the principal component analysis (PCA) and cluster analysis. Both PCA and cluster analysis showed that these 5 saccharides can be used as markers in the steaming process of the P. rhizoma. Present study of mass spectrometry imaging provides novel insights into the spatiotemporal accumulation patterns of saccharides in P. rhizoma, improving our understanding of the steaming process. |
| format | Article |
| id | doaj-art-22d8f1dc3b064cab9683a021aa50582b |
| institution | DOAJ |
| issn | 2097-0765 2213-4530 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-22d8f1dc3b064cab9683a021aa50582b2025-08-20T02:41:14ZengTsinghua University PressFood Science and Human Wellness2097-07652213-45302025-04-01144925009010.26599/FSHW.2024.9250090Identification of markers of saccharides in Polygonati rhizoma during the steaming process by MALDI-Q-TOF mass spectrometry imagingChuang Liu0Juan Chen1Jia Lao2You Qin3Ziling Wang4Zhenni Xie5Wei He6Can Zhong7Shuihan Zhang8Jian Jin9Institute of Chinese Medicine Resources, Hunan Academy of Chinese Medicine, Changsha 410013, ChinaInstitute of Chinese Medicine Resources, Hunan Academy of Chinese Medicine, Changsha 410013, ChinaResgreen Group International Inc., Changsha 410329, ChinaInstitute of Chinese Medicine Resources, Hunan Academy of Chinese Medicine, Changsha 410013, ChinaInstitute of Chinese Medicine Resources, Hunan Academy of Chinese Medicine, Changsha 410013, ChinaInstitute of Chinese Medicine Resources, Hunan Academy of Chinese Medicine, Changsha 410013, ChinaResgreen Group International Inc., Changsha 410329, ChinaInstitute of Chinese Medicine Resources, Hunan Academy of Chinese Medicine, Changsha 410013, ChinaInstitute of Chinese Medicine Resources, Hunan Academy of Chinese Medicine, Changsha 410013, ChinaInstitute of Chinese Medicine Resources, Hunan Academy of Chinese Medicine, Changsha 410013, ChinaPolygonati rhizoma is often used in Chinese medicine and as food. In this study, atmospheric pressure matrix-assisted laser desorption ionization and quadruple-time-of-flight (MALDI-Q-TOF) mass spectrometry techniques were applied to P. rhizoma samples from Polygonatum cyrtonema Hua species. Positive ions were mainly detected in the mass range of m/z 200–600, while negative ions were mainly observed in the mass range of m/z 100–450. A total of 263 components were identified and the spatial distribution and changes in saccharides contents during the steaming process of P. rhizoma were investigated. Monosaccharide and disaccharide exhibit a relatively uniform distribution, while the oligosaccharides were mainly found in the bast of fresh P. rhizoma. Although the contents of monosaccharide and disaccharide were increased during steaming, that of trisaccharide, tetrasaccharide, and pentasaccharide were decreased. We used the 5 saccharide types with the greatest variation in content as variables for the principal component analysis (PCA) and cluster analysis. Both PCA and cluster analysis showed that these 5 saccharides can be used as markers in the steaming process of the P. rhizoma. Present study of mass spectrometry imaging provides novel insights into the spatiotemporal accumulation patterns of saccharides in P. rhizoma, improving our understanding of the steaming process.https://www.sciopen.com/article/10.26599/FSHW.2024.9250090polygonati rhizomemass spectrometry imagingsaccharidesprocess by steamingmarker |
| spellingShingle | Chuang Liu Juan Chen Jia Lao You Qin Ziling Wang Zhenni Xie Wei He Can Zhong Shuihan Zhang Jian Jin Identification of markers of saccharides in Polygonati rhizoma during the steaming process by MALDI-Q-TOF mass spectrometry imaging Food Science and Human Wellness polygonati rhizome mass spectrometry imaging saccharides process by steaming marker |
| title | Identification of markers of saccharides in Polygonati rhizoma during the steaming process by MALDI-Q-TOF mass spectrometry imaging |
| title_full | Identification of markers of saccharides in Polygonati rhizoma during the steaming process by MALDI-Q-TOF mass spectrometry imaging |
| title_fullStr | Identification of markers of saccharides in Polygonati rhizoma during the steaming process by MALDI-Q-TOF mass spectrometry imaging |
| title_full_unstemmed | Identification of markers of saccharides in Polygonati rhizoma during the steaming process by MALDI-Q-TOF mass spectrometry imaging |
| title_short | Identification of markers of saccharides in Polygonati rhizoma during the steaming process by MALDI-Q-TOF mass spectrometry imaging |
| title_sort | identification of markers of saccharides in polygonati rhizoma during the steaming process by maldi q tof mass spectrometry imaging |
| topic | polygonati rhizome mass spectrometry imaging saccharides process by steaming marker |
| url | https://www.sciopen.com/article/10.26599/FSHW.2024.9250090 |
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