Improved Isolation of Ultra-High-Molecular-Weight Genomic DNA Suitable for Third-Generation Sequencing

Improved Isolation of Ultra-High-Molecular-Weight Genomic DNA Suitable for Third-Generation Sequencing

Although a variety of protocols to isolate high-molecular-weight genomic DNA exist, the isolation and preservation of ultra-high-molecular-weight genomic DNA of sufficient quality and length for error-free third-generation sequencing remains challenging. Inspired by the isolation of high-molecular-w...

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Bibliographic Details
Main Authors: Ayşe Öykü Ova, Enrique Joffre, Reza Zandi Shafagh, Mariana F. G. Assunção, Roman Y. Sidorov, Lilia M. A. Santos, Volker M. Lauschke, Ute Römling
Format: Article
Language:English
Published: MDPI AG 2025-02-01
Series:Microorganisms
Subjects:
bacteria
DNA isolation
incubation chamber
third-generation sequencing
Online Access:https://www.mdpi.com/2076-2607/13/3/534
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