Comprehensive approaches to design efficient gRNA for SDN1-CRISPR/Cas9 genome editing in wheat

BackgroundCRISPR/Cas9 technology has gained popularity due to its efficient, widely applicable, and relatively easy genome editing. Furthermore, the removal of regulation on site-directed nuclease1- (SDN1) and SDN2-developed products in many countries has made it a more revolutionary technology for...

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Bibliographic Details
Main Authors: Tushadri Singh, H. M. Mamrutha, Rajender Singh, J. P. Jaiswal, Zeenat Wadhwa, Rakesh Kumar, Omvir Singh, O. P. Ahlawat, Ratan Tiwari
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-07-01
Series:Frontiers in Genome Editing
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Online Access:https://www.frontiersin.org/articles/10.3389/fgeed.2025.1579165/full
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