Protocol for assessing T cell receptor-mediated human T cell cytotoxicity
Summary: Measuring the cytotoxicity of human T cells is a valuable but challenging task. We present a protocol for assessing the ability of polyclonal human T cells to kill target cells upon T cell receptor (TCR) triggering. We describe steps for preparing L and T cells for the assay and incubation...
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Main Authors: | Kathryne E. Marks, Ifeoluwakiisi Adejoorin, John Sowerby, Deepak A. Rao |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2025-03-01
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Series: | STAR Protocols |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166724007068 |
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