Expression and culture optimization of mini-chaperone GroEL (191-345) in E. coli
The apical domain of GroEL (residues 191-345) is expressed in E. coli to give a functional mini-chaperone, and the refolding yields of scorpion toxin Cn5, cyclophilin A and IGPS assisted by mini-chaperone were improved remarkably. Owing to its potentially broad application, the optimum culture condi...
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| Main Authors: | ZHANG Jia-yi, GUAN Yi-xin, YAO Shan-jing |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2003-11-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2003.06.0603 |
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