Expression and culture optimization of mini-chaperone GroEL (191-345) in E. coli

The apical domain of GroEL (residues 191-345) is expressed in E. coli to give a functional mini-chaperone, and the refolding yields of scorpion toxin Cn5, cyclophilin A and IGPS assisted by mini-chaperone were improved remarkably. Owing to its potentially broad application, the optimum culture condi...

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Bibliographic Details
Main Authors: ZHANG Jia-yi, GUAN Yi-xin, YAO Shan-jing
Format: Article
Language:English
Published: Zhejiang University Press 2003-11-01
Series:浙江大学学报. 农业与生命科学版
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Online Access:https://www.academax.com/doi/10.3785/1008-9209.2003.06.0603
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