Expression and culture optimization of mini-chaperone GroEL (191-345) in E. coli
The apical domain of GroEL (residues 191-345) is expressed in E. coli to give a functional mini-chaperone, and the refolding yields of scorpion toxin Cn5, cyclophilin A and IGPS assisted by mini-chaperone were improved remarkably. Owing to its potentially broad application, the optimum culture condi...
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| Language: | English |
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Zhejiang University Press
2003-11-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
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| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2003.06.0603 |
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| author | ZHANG Jia-yi GUAN Yi-xin YAO Shan-jing |
| author_facet | ZHANG Jia-yi GUAN Yi-xin YAO Shan-jing |
| author_sort | ZHANG Jia-yi |
| collection | DOAJ |
| description | The apical domain of GroEL (residues 191-345) is expressed in E. coli to give a functional mini-chaperone, and the refolding yields of scorpion toxin Cn5, cyclophilin A and IGPS assisted by mini-chaperone were improved remarkably. Owing to its potentially broad application, the optimum culture condition of mini-chaperone was studied in detail. M9 medium with carbon source and inorganic salts was found to be suitable for the expression of mini-chaperone GroEL (191-345) in E. coli. M9 medium was then reformulated and the optimum temperature, oxygen demand and induction conditions were carefully chosen to improve the mini-chaperone production to 556.3 mg/L. |
| format | Article |
| id | doaj-art-217d989899374883ab8a17db64d7a48e |
| institution | DOAJ |
| issn | 1008-9209 2097-5155 |
| language | English |
| publishDate | 2003-11-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | 浙江大学学报. 农业与生命科学版 |
| spelling | doaj-art-217d989899374883ab8a17db64d7a48e2025-08-20T02:47:40ZengZhejiang University Press浙江大学学报. 农业与生命科学版1008-92092097-51552003-11-012960360810.3785/1008-9209.2003.06.060310089209Expression and culture optimization of mini-chaperone GroEL (191-345) in E. coliZHANG Jia-yiGUAN Yi-xinYAO Shan-jingThe apical domain of GroEL (residues 191-345) is expressed in E. coli to give a functional mini-chaperone, and the refolding yields of scorpion toxin Cn5, cyclophilin A and IGPS assisted by mini-chaperone were improved remarkably. Owing to its potentially broad application, the optimum culture condition of mini-chaperone was studied in detail. M9 medium with carbon source and inorganic salts was found to be suitable for the expression of mini-chaperone GroEL (191-345) in E. coli. M9 medium was then reformulated and the optimum temperature, oxygen demand and induction conditions were carefully chosen to improve the mini-chaperone production to 556.3 mg/L.https://www.academax.com/doi/10.3785/1008-9209.2003.06.0603mini-chaperoneculture optimizationGroEfermentation |
| spellingShingle | ZHANG Jia-yi GUAN Yi-xin YAO Shan-jing Expression and culture optimization of mini-chaperone GroEL (191-345) in E. coli 浙江大学学报. 农业与生命科学版 mini-chaperone culture optimization GroE fermentation |
| title | Expression and culture optimization of mini-chaperone GroEL (191-345) in E. coli |
| title_full | Expression and culture optimization of mini-chaperone GroEL (191-345) in E. coli |
| title_fullStr | Expression and culture optimization of mini-chaperone GroEL (191-345) in E. coli |
| title_full_unstemmed | Expression and culture optimization of mini-chaperone GroEL (191-345) in E. coli |
| title_short | Expression and culture optimization of mini-chaperone GroEL (191-345) in E. coli |
| title_sort | expression and culture optimization of mini chaperone groel 191 345 in e coli |
| topic | mini-chaperone culture optimization GroE fermentation |
| url | https://www.academax.com/doi/10.3785/1008-9209.2003.06.0603 |
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