The Probiotication of a Lychee Beverage with <i>Saccharomyces boulardii</i>: An Alternative to Dairy-Based Probiotic Products

Probiotic vegetable-based beverages, such as lychee, can be rich in nutrients, free of cholesterol and lactose, and also have few allergenic components. <i>Saccharomyces boulardii</i> is an alternative to make lychee juice a probiotic beverage. This work aimed to develop probiotic lychee...

Full description

Saved in:
Bibliographic Details
Main Authors: Marcela Moreira Terhaag, Otávio Akira Sakai, Fabiana Ruiz, Sandra Garcia, Fernando Rodrigo Bertusso, Sandra Helena Prudêncio
Format: Article
Language:English
Published: MDPI AG 2025-01-01
Series:Foods
Subjects:
Online Access:https://www.mdpi.com/2304-8158/14/2/156
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1832588467455918080
author Marcela Moreira Terhaag
Otávio Akira Sakai
Fabiana Ruiz
Sandra Garcia
Fernando Rodrigo Bertusso
Sandra Helena Prudêncio
author_facet Marcela Moreira Terhaag
Otávio Akira Sakai
Fabiana Ruiz
Sandra Garcia
Fernando Rodrigo Bertusso
Sandra Helena Prudêncio
author_sort Marcela Moreira Terhaag
collection DOAJ
description Probiotic vegetable-based beverages, such as lychee, can be rich in nutrients, free of cholesterol and lactose, and also have few allergenic components. <i>Saccharomyces boulardii</i> is an alternative to make lychee juice a probiotic beverage. This work aimed to develop probiotic lychee beverage (LB) using <i>S. boulardii</i> by evaluating the effect of refrigerated storage on cell viability, physicochemical characteristics, and acceptance. LB supplemented with <i>S. boulardii</i> was fermented (24 h/30 °C), supplemented with sucrose (4–12 °Brix), and refrigerated (up to 28 days/4 °C). The yeast viability, total soluble solid (TSS) and, ethanol content, pH, total phenolic compounds (TPC), and antioxidant activity (AA) levels were evaluated over 28 days of storage. Also, the profiles of sugars, organic acids, and phenolic were determined via chromatographic analysis. The sensory acceptance of the probiotic beverage was evaluated. Higher sucrose levels (12 °Brix) resulted in greater yeast viability (6.9 log CFU/mL) on the 21st day of storage and superior TPC (153 µmol TEAC/mL) and ethanol levels (8.7%). Storage reduced the TPC, AA, and TSS. LB supplemented with sucrose to 12 °Brix, probioticated by <i>S. boulardii</i>, and stored for 21 days became accepted by the consumer and presented an adequate physicochemical profile with probiotic potential. The probiotication of lychee beverage is an alternative to dairy-based probiotic beverages.
format Article
id doaj-art-21252dc4b6ff43749b95ceb7ac79262e
institution Kabale University
issn 2304-8158
language English
publishDate 2025-01-01
publisher MDPI AG
record_format Article
series Foods
spelling doaj-art-21252dc4b6ff43749b95ceb7ac79262e2025-01-24T13:32:40ZengMDPI AGFoods2304-81582025-01-0114215610.3390/foods14020156The Probiotication of a Lychee Beverage with <i>Saccharomyces boulardii</i>: An Alternative to Dairy-Based Probiotic ProductsMarcela Moreira Terhaag0Otávio Akira Sakai1Fabiana Ruiz2Sandra Garcia3Fernando Rodrigo Bertusso4Sandra Helena Prudêncio5Federal Institute of Paraná (IFPR), Umuarama 87507-014, BrazilFederal Institute of Paraná (IFPR), Umuarama 87507-014, BrazilFederal Institute of Paraná (IFPR), Umuarama 87507-014, BrazilDepartment of Food Science and Technology, Londrina State University, Londrina 86051-970, BrazilState Secretariat of Education of Paraná, UNIALFA-Umuarama, Umuarama 87502-000, BrazilDepartment of Food Science and Technology, Londrina State University, Londrina 86051-970, BrazilProbiotic vegetable-based beverages, such as lychee, can be rich in nutrients, free of cholesterol and lactose, and also have few allergenic components. <i>Saccharomyces boulardii</i> is an alternative to make lychee juice a probiotic beverage. This work aimed to develop probiotic lychee beverage (LB) using <i>S. boulardii</i> by evaluating the effect of refrigerated storage on cell viability, physicochemical characteristics, and acceptance. LB supplemented with <i>S. boulardii</i> was fermented (24 h/30 °C), supplemented with sucrose (4–12 °Brix), and refrigerated (up to 28 days/4 °C). The yeast viability, total soluble solid (TSS) and, ethanol content, pH, total phenolic compounds (TPC), and antioxidant activity (AA) levels were evaluated over 28 days of storage. Also, the profiles of sugars, organic acids, and phenolic were determined via chromatographic analysis. The sensory acceptance of the probiotic beverage was evaluated. Higher sucrose levels (12 °Brix) resulted in greater yeast viability (6.9 log CFU/mL) on the 21st day of storage and superior TPC (153 µmol TEAC/mL) and ethanol levels (8.7%). Storage reduced the TPC, AA, and TSS. LB supplemented with sucrose to 12 °Brix, probioticated by <i>S. boulardii</i>, and stored for 21 days became accepted by the consumer and presented an adequate physicochemical profile with probiotic potential. The probiotication of lychee beverage is an alternative to dairy-based probiotic beverages.https://www.mdpi.com/2304-8158/14/2/156juicefermentationyeaststoragefunctional<i>Litchi chinensis</i>
spellingShingle Marcela Moreira Terhaag
Otávio Akira Sakai
Fabiana Ruiz
Sandra Garcia
Fernando Rodrigo Bertusso
Sandra Helena Prudêncio
The Probiotication of a Lychee Beverage with <i>Saccharomyces boulardii</i>: An Alternative to Dairy-Based Probiotic Products
Foods
juice
fermentation
yeast
storage
functional
<i>Litchi chinensis</i>
title The Probiotication of a Lychee Beverage with <i>Saccharomyces boulardii</i>: An Alternative to Dairy-Based Probiotic Products
title_full The Probiotication of a Lychee Beverage with <i>Saccharomyces boulardii</i>: An Alternative to Dairy-Based Probiotic Products
title_fullStr The Probiotication of a Lychee Beverage with <i>Saccharomyces boulardii</i>: An Alternative to Dairy-Based Probiotic Products
title_full_unstemmed The Probiotication of a Lychee Beverage with <i>Saccharomyces boulardii</i>: An Alternative to Dairy-Based Probiotic Products
title_short The Probiotication of a Lychee Beverage with <i>Saccharomyces boulardii</i>: An Alternative to Dairy-Based Probiotic Products
title_sort probiotication of a lychee beverage with i saccharomyces boulardii i an alternative to dairy based probiotic products
topic juice
fermentation
yeast
storage
functional
<i>Litchi chinensis</i>
url https://www.mdpi.com/2304-8158/14/2/156
work_keys_str_mv AT marcelamoreiraterhaag theprobioticationofalycheebeveragewithisaccharomycesboulardiiianalternativetodairybasedprobioticproducts
AT otavioakirasakai theprobioticationofalycheebeveragewithisaccharomycesboulardiiianalternativetodairybasedprobioticproducts
AT fabianaruiz theprobioticationofalycheebeveragewithisaccharomycesboulardiiianalternativetodairybasedprobioticproducts
AT sandragarcia theprobioticationofalycheebeveragewithisaccharomycesboulardiiianalternativetodairybasedprobioticproducts
AT fernandorodrigobertusso theprobioticationofalycheebeveragewithisaccharomycesboulardiiianalternativetodairybasedprobioticproducts
AT sandrahelenaprudencio theprobioticationofalycheebeveragewithisaccharomycesboulardiiianalternativetodairybasedprobioticproducts
AT marcelamoreiraterhaag probioticationofalycheebeveragewithisaccharomycesboulardiiianalternativetodairybasedprobioticproducts
AT otavioakirasakai probioticationofalycheebeveragewithisaccharomycesboulardiiianalternativetodairybasedprobioticproducts
AT fabianaruiz probioticationofalycheebeveragewithisaccharomycesboulardiiianalternativetodairybasedprobioticproducts
AT sandragarcia probioticationofalycheebeveragewithisaccharomycesboulardiiianalternativetodairybasedprobioticproducts
AT fernandorodrigobertusso probioticationofalycheebeveragewithisaccharomycesboulardiiianalternativetodairybasedprobioticproducts
AT sandrahelenaprudencio probioticationofalycheebeveragewithisaccharomycesboulardiiianalternativetodairybasedprobioticproducts