Forensic Body Fluid Identification by Analysis of Multiple RNA Markers Using NanoString Technology
RNA analysis has become a reliable method of body fluid identification for forensic use. Previously, we developed a combination of four multiplex quantitative PCR (qRT-PCR) probes to discriminate four different body fluids (blood, semen, saliva, and vaginal secretion). While those makers successfull...
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| Format: | Article |
| Language: | English |
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BioMed Central
2013-12-01
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| Series: | Genomics & Informatics |
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| Online Access: | http://genominfo.org/upload/pdf/gni-11-277.pdf |
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| author | Jong-Lyul Park Seong-Min Park Jeong-Hwan Kim Han-Chul Lee Seung-Hwan Lee Kwang-Man Woo Seon-Young Kim |
| author_facet | Jong-Lyul Park Seong-Min Park Jeong-Hwan Kim Han-Chul Lee Seung-Hwan Lee Kwang-Man Woo Seon-Young Kim |
| author_sort | Jong-Lyul Park |
| collection | DOAJ |
| description | RNA analysis has become a reliable method of body fluid identification for forensic use. Previously, we developed a combination of four multiplex quantitative PCR (qRT-PCR) probes to discriminate four different body fluids (blood, semen, saliva, and vaginal secretion). While those makers successfully identified most body fluid samples, there were some cases of false positive and negative identification. To improve the accuracy of the identification further, we tried to use multiple markers per body fluid and adopted the NanoString nCounter system instead of a multiplex qRT-PCR system. After measuring tens of RNA markers, we evaluated the accuracy of each marker for body fluid identification. For body fluids, such as blood and semen, each body fluid-specific marker was accurate enough for perfect identification. However, for saliva and vaginal secretion, no single marker was perfect. Thus, we designed a logistic regression model with multiple markers for saliva and vaginal secretion and achieved almost perfect identification. In conclusion, the NanoString nCounter is an efficient platform for measuring multiple RNA markers per body fluid and will be useful for forensic RNA analysis. |
| format | Article |
| id | doaj-art-21077f75fa2a482a9558525343d5cb39 |
| institution | Kabale University |
| issn | 1598-866X 2234-0742 |
| language | English |
| publishDate | 2013-12-01 |
| publisher | BioMed Central |
| record_format | Article |
| series | Genomics & Informatics |
| spelling | doaj-art-21077f75fa2a482a9558525343d5cb392025-02-02T03:46:20ZengBioMed CentralGenomics & Informatics1598-866X2234-07422013-12-0111427728110.5808/GI.2013.11.4.27770Forensic Body Fluid Identification by Analysis of Multiple RNA Markers Using NanoString TechnologyJong-Lyul Park0Seong-Min Park1Jeong-Hwan Kim2Han-Chul Lee3Seung-Hwan Lee4Kwang-Man Woo5Seon-Young Kim6Medical Genomics Research Center, KRIBB, Daejeon 305-806, Korea.Medical Genomics Research Center, KRIBB, Daejeon 305-806, Korea.Medical Genomics Research Center, KRIBB, Daejeon 305-806, Korea.DNA orensic Division, Supreme Prosecutor's Office, Seoul 137-730, Korea.DNA orensic Division, Supreme Prosecutor's Office, Seoul 137-730, Korea.DNA orensic Division, Supreme Prosecutor's Office, Seoul 137-730, Korea.Medical Genomics Research Center, KRIBB, Daejeon 305-806, Korea.RNA analysis has become a reliable method of body fluid identification for forensic use. Previously, we developed a combination of four multiplex quantitative PCR (qRT-PCR) probes to discriminate four different body fluids (blood, semen, saliva, and vaginal secretion). While those makers successfully identified most body fluid samples, there were some cases of false positive and negative identification. To improve the accuracy of the identification further, we tried to use multiple markers per body fluid and adopted the NanoString nCounter system instead of a multiplex qRT-PCR system. After measuring tens of RNA markers, we evaluated the accuracy of each marker for body fluid identification. For body fluids, such as blood and semen, each body fluid-specific marker was accurate enough for perfect identification. However, for saliva and vaginal secretion, no single marker was perfect. Thus, we designed a logistic regression model with multiple markers for saliva and vaginal secretion and achieved almost perfect identification. In conclusion, the NanoString nCounter is an efficient platform for measuring multiple RNA markers per body fluid and will be useful for forensic RNA analysis.http://genominfo.org/upload/pdf/gni-11-277.pdfbody fluidsforensicRNA |
| spellingShingle | Jong-Lyul Park Seong-Min Park Jeong-Hwan Kim Han-Chul Lee Seung-Hwan Lee Kwang-Man Woo Seon-Young Kim Forensic Body Fluid Identification by Analysis of Multiple RNA Markers Using NanoString Technology Genomics & Informatics body fluids forensic RNA |
| title | Forensic Body Fluid Identification by Analysis of Multiple RNA Markers Using NanoString Technology |
| title_full | Forensic Body Fluid Identification by Analysis of Multiple RNA Markers Using NanoString Technology |
| title_fullStr | Forensic Body Fluid Identification by Analysis of Multiple RNA Markers Using NanoString Technology |
| title_full_unstemmed | Forensic Body Fluid Identification by Analysis of Multiple RNA Markers Using NanoString Technology |
| title_short | Forensic Body Fluid Identification by Analysis of Multiple RNA Markers Using NanoString Technology |
| title_sort | forensic body fluid identification by analysis of multiple rna markers using nanostring technology |
| topic | body fluids forensic RNA |
| url | http://genominfo.org/upload/pdf/gni-11-277.pdf |
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