Physicochemical investigation of phenobarbital sodium interactions with anionic bile salt micelles: Binding, partitioning, and micellization behavior

Understanding drug-surfactant interactions is crucial for optimizing pharmaceutical formulations. This study aimed to comprehensively investigate the physicochemical interactions between phenobarbital sodium (PS) and two anionic bile salts, sodium cholate (NaC) and sodium deoxycholate (NaDC), in aqu...

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Main Authors: Haytham Abuissa, Ashraf EL-Hashani
Format: Article
Language:English
Published: Elsevier 2025-07-01
Series:Next Materials
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Online Access:http://www.sciencedirect.com/science/article/pii/S2949822825003922
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author Haytham Abuissa
Ashraf EL-Hashani
author_facet Haytham Abuissa
Ashraf EL-Hashani
author_sort Haytham Abuissa
collection DOAJ
description Understanding drug-surfactant interactions is crucial for optimizing pharmaceutical formulations. This study aimed to comprehensively investigate the physicochemical interactions between phenobarbital sodium (PS) and two anionic bile salts, sodium cholate (NaC) and sodium deoxycholate (NaDC), in aqueous media. UV-Vis spectroscopy, conductivity, surface tension, and viscosity measurements were employed to determine critical micelle concentration (CMC), binding constants (Kb), partition coefficients (Kx), interfacial parameters, and thermodynamic functions. Key results show PS significantly reduced the CMC of both bile salts (NaC: 1.30 ×10⁻⁴ M; NaDC: 1.92 ×10⁻⁵ M). NaDC consistently demonstrated superior surface activity (e.g., Γmax = 1.958 µmol·m⁻², pC20 = 4.81) and more favorable thermodynamics for micellization (ΔG°m = −64.79 kJ/mol) and adsorption (ΔG°ads = −78.17 kJ/mol) compared to NaC. Furthermore, NaDC exhibited significantly stronger PS binding (Kb ≈ 1.44 ×10⁶ M⁻¹) and partitioning (Kx ≈ 2.85 ×10⁶), encapsulating ∼14 times more PS molecules per micelle than NaC. In conclusion, NaDC's enhanced hydrophobicity leads to superior micellization, drug binding, and solubilization capabilities, highlighting its considerable potential as an effective biosurfactant carrier for phenobarbital in pharmaceutical applications, offering valuable insights for drug delivery design.
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spelling doaj-art-20cce00a8c344d2f83e25700b134f9942025-08-20T03:32:47ZengElsevierNext Materials2949-82282025-07-01810087410.1016/j.nxmate.2025.100874Physicochemical investigation of phenobarbital sodium interactions with anionic bile salt micelles: Binding, partitioning, and micellization behaviorHaytham Abuissa0Ashraf EL-Hashani1Chemistry Department, Faculty of Arts and Science, Gheminus, Benghazi University, Gheminus, LibyaChemistry Department, Faculty of Science, Benghazi University, Benghazi, Libya; Corresponding author.Understanding drug-surfactant interactions is crucial for optimizing pharmaceutical formulations. This study aimed to comprehensively investigate the physicochemical interactions between phenobarbital sodium (PS) and two anionic bile salts, sodium cholate (NaC) and sodium deoxycholate (NaDC), in aqueous media. UV-Vis spectroscopy, conductivity, surface tension, and viscosity measurements were employed to determine critical micelle concentration (CMC), binding constants (Kb), partition coefficients (Kx), interfacial parameters, and thermodynamic functions. Key results show PS significantly reduced the CMC of both bile salts (NaC: 1.30 ×10⁻⁴ M; NaDC: 1.92 ×10⁻⁵ M). NaDC consistently demonstrated superior surface activity (e.g., Γmax = 1.958 µmol·m⁻², pC20 = 4.81) and more favorable thermodynamics for micellization (ΔG°m = −64.79 kJ/mol) and adsorption (ΔG°ads = −78.17 kJ/mol) compared to NaC. Furthermore, NaDC exhibited significantly stronger PS binding (Kb ≈ 1.44 ×10⁶ M⁻¹) and partitioning (Kx ≈ 2.85 ×10⁶), encapsulating ∼14 times more PS molecules per micelle than NaC. In conclusion, NaDC's enhanced hydrophobicity leads to superior micellization, drug binding, and solubilization capabilities, highlighting its considerable potential as an effective biosurfactant carrier for phenobarbital in pharmaceutical applications, offering valuable insights for drug delivery design.http://www.sciencedirect.com/science/article/pii/S2949822825003922Bile saltsCritical Micelle Concentration (CMC)Interfacial parametersBinding constantPartition coefficient
spellingShingle Haytham Abuissa
Ashraf EL-Hashani
Physicochemical investigation of phenobarbital sodium interactions with anionic bile salt micelles: Binding, partitioning, and micellization behavior
Next Materials
Bile salts
Critical Micelle Concentration (CMC)
Interfacial parameters
Binding constant
Partition coefficient
title Physicochemical investigation of phenobarbital sodium interactions with anionic bile salt micelles: Binding, partitioning, and micellization behavior
title_full Physicochemical investigation of phenobarbital sodium interactions with anionic bile salt micelles: Binding, partitioning, and micellization behavior
title_fullStr Physicochemical investigation of phenobarbital sodium interactions with anionic bile salt micelles: Binding, partitioning, and micellization behavior
title_full_unstemmed Physicochemical investigation of phenobarbital sodium interactions with anionic bile salt micelles: Binding, partitioning, and micellization behavior
title_short Physicochemical investigation of phenobarbital sodium interactions with anionic bile salt micelles: Binding, partitioning, and micellization behavior
title_sort physicochemical investigation of phenobarbital sodium interactions with anionic bile salt micelles binding partitioning and micellization behavior
topic Bile salts
Critical Micelle Concentration (CMC)
Interfacial parameters
Binding constant
Partition coefficient
url http://www.sciencedirect.com/science/article/pii/S2949822825003922
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